Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes
Нема приказа
Аутори
Bajić, VladanStanimirović, Zoran
Stevanović, Jevrosima
Potparević, Biljana
Živković, Lada
Milicević, Z.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of re...sponse when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.
Кључне речи:
8-Cl-cAMP / antitumor agents / micronuclei / premature centromere separationИзвор:
Journal of BUON, 2009, 14, 1, 71-77Издавач:
- Balkan Union of Oncology (B.U.ON.)
Финансирање / пројекти:
Институција/група
PharmacyTY - JOUR AU - Bajić, Vladan AU - Stanimirović, Zoran AU - Stevanović, Jevrosima AU - Potparević, Biljana AU - Živković, Lada AU - Milicević, Z. PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1233 AB - Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents. PB - Balkan Union of Oncology (B.U.ON.) T2 - Journal of BUON T1 - Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes VL - 14 IS - 1 SP - 71 EP - 77 UR - https://hdl.handle.net/21.15107/rcub_vinar_3670 ER -
@article{ author = "Bajić, Vladan and Stanimirović, Zoran and Stevanović, Jevrosima and Potparević, Biljana and Živković, Lada and Milicević, Z.", year = "2009", abstract = "Purpose: To assess the cytogenetic effects in vitro and in vivo of a non-cytotoxic antitumor agent with biomodulator activity, 8-chloro-3', 5' cyclic adenosine monophosphate (8-Cl-cAMP). Materials and methods: Cytogenetic effects of 8-Cl-cAMP where evaluated using the in vitro chromosome cytogenetic assail (CA) on human peripheral blood lymphocytes of healthy individuals and by hone marrow micronucleus assay in adult BALB/c mice. Results: In the in vitro chromosome CA, 8-Cl-cAMP (in all respective doses; 1.5 and 15 pin) induced mitotic inhibition and premature centromere separation (PCS) but no chromosomal damage in cultured human peripheral blood lymphocytes. In the in vivo test, single intraperitoneal (i.p) injection of 8-Cl-cAMP in doses of 10, 80 and 15 0 mg/kg showed a dose-related effect on the frequency of micronuclei, detected in murine polychromatic erythrocytes (PCE). Conclusion: The results of the present study show that genotoxicity of 8-Cl-cAMP has a different matrix of response when comparing results in vitro and in vivo, suggesting that high metabolic activity in vivo is responsible for the clastogenic potential of 8-Cl-cAMP These comparative results indicate a need of having an available battery of genotoxic tests in order to evaluate possible cytogenetic effects of novel antitumor agents.", publisher = "Balkan Union of Oncology (B.U.ON.)", journal = "Journal of BUON", title = "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes", volume = "14", number = "1", pages = "71-77", url = "https://hdl.handle.net/21.15107/rcub_vinar_3670" }
Bajić, V., Stanimirović, Z., Stevanović, J., Potparević, B., Živković, L.,& Milicević, Z.. (2009). Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON Balkan Union of Oncology (B.U.ON.)., 14(1), 71-77. https://hdl.handle.net/21.15107/rcub_vinar_3670
Bajić V, Stanimirović Z, Stevanović J, Potparević B, Živković L, Milicević Z. Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes. in Journal of BUON. 2009;14(1):71-77. https://hdl.handle.net/21.15107/rcub_vinar_3670 .
Bajić, Vladan, Stanimirović, Zoran, Stevanović, Jevrosima, Potparević, Biljana, Živković, Lada, Milicević, Z., "Cytogenetic effects of 8-Cl-cAMP on human and animal chromosomes" in Journal of BUON, 14, no. 1 (2009):71-77, https://hdl.handle.net/21.15107/rcub_vinar_3670 .