Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes
Insulin mimetički efekat niskih koncentracija amonijum dekavanadata na izolovane adipocite pacova
Апстракт
In this study, the insulin mimetic activity of ammonium decavanadate (DV) (in concentration 0.1 and 1.0 mM) dissolved in saline (0.9 % w/v of NaCl) or dimethyl sulfoxide, DMSO (2% v/v) was evaluated by means of in vitro measurements of the glucose uptake (GU) and inhibition of free fatty acids release (IFFAR) using isolated epinephrine-pretreated white adipocytes from Wistar rats. Our data showed strong insulin mimetic effect of DV (> 80 %) in both concentration and independently of the solvent were used. Having in mind satisfactory pharmacological effect of DMSO as well as its capability of penetration enhancer it would be reasonable to continue in vivo testing of dose-dependent insulin mimetic effect of decavanadate in DMSO. It is also necessary to examine toxicity of decavanadate in order to estimate its therapeutic index as potential antidiabetic agent.
Ispitana je insulin mimetička aktivnost amonijum dekavanadata (DV), in vitro merenjem preuzimanja glukoze (GU) i merenjem inhibicije oslobađanja slobodnih masnih kiselina (IFFAR) na modelu izolovanih belih adipocita Wistar pacova. Ovaj biološki efekat dekavanadata (u koncentracijama od 0,1 i 1,0 mM) koji je rastvoren u fiziološkom rastvoru (0.9 % NaCl) ili u dimetil sulfoksidu (2% DMSO) ispitan je na adipocitima koji su prethodno tretirani adrenalinom. Prema dobijenim rezultatima dekavanadat pokazuje snažan insulin mimetički efekat (> 80 %) i to u obe koncentracije i nezavisno od vrste rastvarača. Na osnovu ovog in vitro eksperimenta može se zaključiti da bi bilo opravdano dozno-zavisno ispitivanje insulin mimetičkog efekta dekavanadata u DMSO-u kao rastvaraču u in vivo uslovima. Takođe, neophodno je ispitati i toksičnost ovog jedinjenja kako bi se što bezbednije iskoristio njegov insulin mimetički efekat.
Кључне речи:
decavanadate / adipocytes / insulin mimetic / dimethyl sulfoxide / dekavanadat / dimetilsulfoksid / insulin mimeticИзвор:
Arhiv za farmaciju, 2010, 60, 1, 1-12Издавач:
- Savez farmaceutskih udruženja Srbije, Beograd
Институција/група
PharmacyTY - JOUR AU - Topić, Aleksandra AU - Milenković, Marina AU - Uskoković-Marković, Snežana AU - Vučićević, Dragana PY - 2010 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1423 AB - In this study, the insulin mimetic activity of ammonium decavanadate (DV) (in concentration 0.1 and 1.0 mM) dissolved in saline (0.9 % w/v of NaCl) or dimethyl sulfoxide, DMSO (2% v/v) was evaluated by means of in vitro measurements of the glucose uptake (GU) and inhibition of free fatty acids release (IFFAR) using isolated epinephrine-pretreated white adipocytes from Wistar rats. Our data showed strong insulin mimetic effect of DV (> 80 %) in both concentration and independently of the solvent were used. Having in mind satisfactory pharmacological effect of DMSO as well as its capability of penetration enhancer it would be reasonable to continue in vivo testing of dose-dependent insulin mimetic effect of decavanadate in DMSO. It is also necessary to examine toxicity of decavanadate in order to estimate its therapeutic index as potential antidiabetic agent. AB - Ispitana je insulin mimetička aktivnost amonijum dekavanadata (DV), in vitro merenjem preuzimanja glukoze (GU) i merenjem inhibicije oslobađanja slobodnih masnih kiselina (IFFAR) na modelu izolovanih belih adipocita Wistar pacova. Ovaj biološki efekat dekavanadata (u koncentracijama od 0,1 i 1,0 mM) koji je rastvoren u fiziološkom rastvoru (0.9 % NaCl) ili u dimetil sulfoksidu (2% DMSO) ispitan je na adipocitima koji su prethodno tretirani adrenalinom. Prema dobijenim rezultatima dekavanadat pokazuje snažan insulin mimetički efekat (> 80 %) i to u obe koncentracije i nezavisno od vrste rastvarača. Na osnovu ovog in vitro eksperimenta može se zaključiti da bi bilo opravdano dozno-zavisno ispitivanje insulin mimetičkog efekta dekavanadata u DMSO-u kao rastvaraču u in vivo uslovima. Takođe, neophodno je ispitati i toksičnost ovog jedinjenja kako bi se što bezbednije iskoristio njegov insulin mimetički efekat. PB - Savez farmaceutskih udruženja Srbije, Beograd T2 - Arhiv za farmaciju T1 - Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes T1 - Insulin mimetički efekat niskih koncentracija amonijum dekavanadata na izolovane adipocite pacova VL - 60 IS - 1 SP - 1 EP - 12 UR - https://hdl.handle.net/21.15107/rcub_farfar_1423 ER -
@article{ author = "Topić, Aleksandra and Milenković, Marina and Uskoković-Marković, Snežana and Vučićević, Dragana", year = "2010", abstract = "In this study, the insulin mimetic activity of ammonium decavanadate (DV) (in concentration 0.1 and 1.0 mM) dissolved in saline (0.9 % w/v of NaCl) or dimethyl sulfoxide, DMSO (2% v/v) was evaluated by means of in vitro measurements of the glucose uptake (GU) and inhibition of free fatty acids release (IFFAR) using isolated epinephrine-pretreated white adipocytes from Wistar rats. Our data showed strong insulin mimetic effect of DV (> 80 %) in both concentration and independently of the solvent were used. Having in mind satisfactory pharmacological effect of DMSO as well as its capability of penetration enhancer it would be reasonable to continue in vivo testing of dose-dependent insulin mimetic effect of decavanadate in DMSO. It is also necessary to examine toxicity of decavanadate in order to estimate its therapeutic index as potential antidiabetic agent., Ispitana je insulin mimetička aktivnost amonijum dekavanadata (DV), in vitro merenjem preuzimanja glukoze (GU) i merenjem inhibicije oslobađanja slobodnih masnih kiselina (IFFAR) na modelu izolovanih belih adipocita Wistar pacova. Ovaj biološki efekat dekavanadata (u koncentracijama od 0,1 i 1,0 mM) koji je rastvoren u fiziološkom rastvoru (0.9 % NaCl) ili u dimetil sulfoksidu (2% DMSO) ispitan je na adipocitima koji su prethodno tretirani adrenalinom. Prema dobijenim rezultatima dekavanadat pokazuje snažan insulin mimetički efekat (> 80 %) i to u obe koncentracije i nezavisno od vrste rastvarača. Na osnovu ovog in vitro eksperimenta može se zaključiti da bi bilo opravdano dozno-zavisno ispitivanje insulin mimetičkog efekta dekavanadata u DMSO-u kao rastvaraču u in vivo uslovima. Takođe, neophodno je ispitati i toksičnost ovog jedinjenja kako bi se što bezbednije iskoristio njegov insulin mimetički efekat.", publisher = "Savez farmaceutskih udruženja Srbije, Beograd", journal = "Arhiv za farmaciju", title = "Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes, Insulin mimetički efekat niskih koncentracija amonijum dekavanadata na izolovane adipocite pacova", volume = "60", number = "1", pages = "1-12", url = "https://hdl.handle.net/21.15107/rcub_farfar_1423" }
Topić, A., Milenković, M., Uskoković-Marković, S.,& Vučićević, D.. (2010). Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes. in Arhiv za farmaciju Savez farmaceutskih udruženja Srbije, Beograd., 60(1), 1-12. https://hdl.handle.net/21.15107/rcub_farfar_1423
Topić A, Milenković M, Uskoković-Marković S, Vučićević D. Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes. in Arhiv za farmaciju. 2010;60(1):1-12. https://hdl.handle.net/21.15107/rcub_farfar_1423 .
Topić, Aleksandra, Milenković, Marina, Uskoković-Marković, Snežana, Vučićević, Dragana, "Low concentration of ammonium decavanadate exhibited insulin mimetic effect on isolated rat adipocytes" in Arhiv za farmaciju, 60, no. 1 (2010):1-12, https://hdl.handle.net/21.15107/rcub_farfar_1423 .