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Gender-related reference intervals of urinary 8-oxo-7,8-dihydro-2 '-deoxyguanosine determined by liquid chromatography-tandem mass spectrometry in Serbian population
dc.creator | Topić, Aleksandra | |
dc.creator | Francuski, Đorđe | |
dc.creator | Marković, Bojan | |
dc.creator | Stanković, Marija | |
dc.creator | Dobrivojević, Snežana | |
dc.creator | Drca, Sanja | |
dc.creator | Radojković, Dragica | |
dc.date.accessioned | 2019-09-02T11:34:12Z | |
dc.date.available | 2019-09-02T11:34:12Z | |
dc.date.issued | 2013 | |
dc.identifier.issn | 0009-9120 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/1917 | |
dc.description.abstract | Objectives: Although there are many nucleobase modifications, 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is one of the dominant form of oxidative modifications of DNA. Urinary 8-oxodG is potentially the best non-invasive biomarker of oxidative stress. Defining reference interval for urinary 8-oxodG is a prerequisite for its clinical use as biomarker. Design and methods: Reference population included 229 healthy Serbian adults (130 males and 99 females). The spot urinary 8-oxodG was determined using high performance liquid chromatography and tandem mass spectrometry (HPLC-MS/MS). Urinary creatinine was measured by the kinetic Jaffe method. Results: Analytical performances of the HPLC-MS/MS: CVs within and between-run variations were 5.6% and 2.6%; LOD and LOQ were 1.65 nmol/L and 330 nmol/L; mean recovery and relative accuracy were 96% and 97%. Creatinine level was higher in males than in females, but no gender difference in 8-oxodG level was observed. Upon the adjustment of 8-oxodG to creatinine (8-oxodG/creatinine), higher values were obtained in females (1.38 +/- 0.65 nmol/mmol) than in males (1.05 +/- 0.48 nmol/mmol). Distribution of 8-oxodG/creatinine in spot urine sample was log-normal and gender-related reference intervals (estimated as the 2.5th-97.5th percentiles) were 0.45-2.22 nmol/mmol for males, and 0.54-3.11 nmol/mmol for females. Body mass index (BMI) affects excretion of the 8-oxodG in males, independently of urinary creatinine, while in females it does not. Therefore, BMI might contribute to the gender-related differences of 8-oxodG/creatinine in spot urine samples. Conclusions: This is the first established gender-related reference intervals of spot urinary 8-oxodG/creatinine. Our results contribute to the full validation of 8-oxodG as biomarker of oxidative stress. | en |
dc.publisher | Pergamon-Elsevier Science Ltd, Oxford | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173008/RS// | |
dc.rights | restrictedAccess | |
dc.source | Clinical Biochemistry | |
dc.subject | 8-oxo-7,8-dihydro-2 '-deoxyguanosine | en |
dc.subject | Oxidative stress | en |
dc.subject | Reference interval | en |
dc.subject | Spot urine | en |
dc.subject | HPLC/MS/MS | en |
dc.title | Gender-related reference intervals of urinary 8-oxo-7,8-dihydro-2 '-deoxyguanosine determined by liquid chromatography-tandem mass spectrometry in Serbian population | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Добривојевић, Снежана; Радојковић, Драгица; Дрца, Сања; Топић, Aлександра; Марковић, Бојан; Француски, Ђорђе; Станковић, Марија; | |
dc.citation.volume | 46 | |
dc.citation.issue | 4-5 | |
dc.citation.spage | 321 | |
dc.citation.epage | 326 | |
dc.citation.other | 46(4-5): 321-326 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000315661700009 | |
dc.identifier.doi | 10.1016/j.clinbiochem.2012.12.008 | |
dc.identifier.pmid | 23261837 | |
dc.identifier.scopus | 2-s2.0-84873731090 | |
dc.type.version | publishedVersion |