Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity
Само за регистроване кориснике
2014
Аутори
Srecković, IvanaBirner-Gruenberger, Ruth
Besenboeck, Carolin
Miljković, Milica
Stojaković, Tatjana
Scharnagl, Hubert
Marsche, Gunther
Lang, Uwe
Kotur-Stevuljević, Jelena
Jelić-Ivanović, Zorana
Desoye, Gernot
Wadsack, Christian
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p lt 0.05) and SAA1 (increased, p lt 0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated ...by lower activity (p lt 0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p lt 0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism.
Кључне речи:
Neonatal HDL / GDM / Anti-oxidative propertyИзвор:
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 2014, 1841, 11, 1619-1627Издавач:
- Elsevier Science BV, Amsterdam
Финансирање / пројекти:
- PhD Program Molecular Medicine of the Medical University of Graz
DOI: 10.1016/j.bbalip.2014.07.021
ISSN: 1388-1981
PubMed: 25130684
WoS: 000343854300006
Scopus: 2-s2.0-84907720790
Институција/група
PharmacyTY - JOUR AU - Srecković, Ivana AU - Birner-Gruenberger, Ruth AU - Besenboeck, Carolin AU - Miljković, Milica AU - Stojaković, Tatjana AU - Scharnagl, Hubert AU - Marsche, Gunther AU - Lang, Uwe AU - Kotur-Stevuljević, Jelena AU - Jelić-Ivanović, Zorana AU - Desoye, Gernot AU - Wadsack, Christian PY - 2014 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2129 AB - Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p lt 0.05) and SAA1 (increased, p lt 0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated by lower activity (p lt 0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p lt 0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism. PB - Elsevier Science BV, Amsterdam T2 - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids T1 - Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity VL - 1841 IS - 11 SP - 1619 EP - 1627 DO - 10.1016/j.bbalip.2014.07.021 ER -
@article{ author = "Srecković, Ivana and Birner-Gruenberger, Ruth and Besenboeck, Carolin and Miljković, Milica and Stojaković, Tatjana and Scharnagl, Hubert and Marsche, Gunther and Lang, Uwe and Kotur-Stevuljević, Jelena and Jelić-Ivanović, Zorana and Desoye, Gernot and Wadsack, Christian", year = "2014", abstract = "Gestational diabetes mellitus (GDM) is related to neonatal macrosomia and an increased risk of vascular events. We hypothesized that GDM exerts qualitative effects on neonatal high-density lipoprotein (HDL). HDL was isolated from control (n = 11) and GDM maternal/neonatal donors (n = 9) and subjected to shotgun proteomics. Differences in HDL mobility were assessed by FPLC and native gel-electrophoresis. Paraoxonase (PON1) activity, cholesterol ester-transfer protein (CETP) mass and activity, phospholipid, triglyceride and cholesterol concentrations were quantified with commercial kits. Total anti-oxidative capacity and cholesterol efflux capability of HDLs were measured. Four proteins involved in lipid metabolism, inflammation and innate immunity were differentially expressed between controls and GDM neonates. ApoM (decreased, p lt 0.05) and SAA1 (increased, p lt 0.05) showed the same differences on both, maternal and neonatal GDM HDL Lower PON1 protein expression was corroborated by lower activity (p lt 0.05) which in turn was associated with attenuated anti-oxidant capacity of GDM HDL Protein changes were accompanied by increased levels of triglycerides and decreased levels of cholesterol esters, respectively. The observed differences in GDM HDL lipid moiety may be related to CETP mass and activity alterations. The rate of cholesterol efflux from term trophoblasts to maternal and from placental endothelial cells to neonatal GDM HDL was impaired (p lt 0.05). In conclusion, GDM causes changes in HDL composition and is intimately associated with impaired cholesterol efflux capability as well as diminished anti-oxidative particle properties. Remodeling of neonatal GDM HDL in utero supports the hypothesis that maternal conditions in pregnancy impact neonatal lipoprotein metabolism.", publisher = "Elsevier Science BV, Amsterdam", journal = "Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids", title = "Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity", volume = "1841", number = "11", pages = "1619-1627", doi = "10.1016/j.bbalip.2014.07.021" }
Srecković, I., Birner-Gruenberger, R., Besenboeck, C., Miljković, M., Stojaković, T., Scharnagl, H., Marsche, G., Lang, U., Kotur-Stevuljević, J., Jelić-Ivanović, Z., Desoye, G.,& Wadsack, C.. (2014). Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity. in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids Elsevier Science BV, Amsterdam., 1841(11), 1619-1627. https://doi.org/10.1016/j.bbalip.2014.07.021
Srecković I, Birner-Gruenberger R, Besenboeck C, Miljković M, Stojaković T, Scharnagl H, Marsche G, Lang U, Kotur-Stevuljević J, Jelić-Ivanović Z, Desoye G, Wadsack C. Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity. in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids. 2014;1841(11):1619-1627. doi:10.1016/j.bbalip.2014.07.021 .
Srecković, Ivana, Birner-Gruenberger, Ruth, Besenboeck, Carolin, Miljković, Milica, Stojaković, Tatjana, Scharnagl, Hubert, Marsche, Gunther, Lang, Uwe, Kotur-Stevuljević, Jelena, Jelić-Ivanović, Zorana, Desoye, Gernot, Wadsack, Christian, "Gestational diabetes mellitus modulates neonatal high-density lipoprotein composition and its functional heterogeneity" in Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1841, no. 11 (2014):1619-1627, https://doi.org/10.1016/j.bbalip.2014.07.021 . .