Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies
Аутори
Varmazyari, AtefehTaghizadehghalehjoughi, Ali
Sevim, Cigdem
Baris, Ozlem
Eser, Gizem
Yildirim, Serkan
Hacimuftuoglu, Ahmet
Buha, Aleksandra
Wallace, David R.
Tsatsakis, Aristidis
Aschner, Michael
Mezhuev, Yaroslav
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Living organisms have an innate ability to regulate the synthesis of inorganic materials, such as bones and teeth in humans. Cadmium sulfide (CdS) can be utilized as a quantum dot that functions as a unique light-emitting semiconductor nanocrystal. The increased use in CdS has led to an increased inhalation and ingestion rate of CdS by humans which requires a broader appreciation for the acute and chronic toxicity of CdS. We investigated the toxic effects of CdS on cerebellar cell cultures and rat brain. We employed a ‘green synthesis’ biosynthesis process to obtain biocompatible material that can be used in living organisms, such as Viridibacillus arenosi K64. Nanocrystal formation was initiated by adding CdCl2 (1 mM) to the cell cultures. Our in vitro results established that increased concentrations of CdS (0.1 μg/mL) lead to decreased cell viability as assessed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), total antioxidant capacity (TAC), and total oxi...dant status (TOS). The in vivo studies showed that exposure to CdS (1 mg/kg) glial fibrillary acidic protein (GFAP) and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were increased. Collectively, we describe a model system that addresses the process from the synthesis to the neurotoxicity assessment for CdS both in vitro and in vivo. These data will be beneficial in establishing a more comprehensive pathway for the understanding of quantum dot-induced neurotoxicity.
Кључне речи:
CdS / Cerebellum neuron / Green synthesis / Neurotoxicity / Quantum dotsИзвор:
Toxicology Reports, 2020, 7, 637-648Издавач:
- Elsevier
Финансирање / пројекти:
- NIEHSR01ES07331
- R01ES10563
- Oklahoma State University grant #154357
DOI: 10.1016/j.toxrep.2020.04.011
ISSN: 2214-7500
WoS: 000604358700004
Scopus: 2-s2.0-85085285759
Институција/група
PharmacyTY - JOUR AU - Varmazyari, Atefeh AU - Taghizadehghalehjoughi, Ali AU - Sevim, Cigdem AU - Baris, Ozlem AU - Eser, Gizem AU - Yildirim, Serkan AU - Hacimuftuoglu, Ahmet AU - Buha, Aleksandra AU - Wallace, David R. AU - Tsatsakis, Aristidis AU - Aschner, Michael AU - Mezhuev, Yaroslav PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3593 AB - Living organisms have an innate ability to regulate the synthesis of inorganic materials, such as bones and teeth in humans. Cadmium sulfide (CdS) can be utilized as a quantum dot that functions as a unique light-emitting semiconductor nanocrystal. The increased use in CdS has led to an increased inhalation and ingestion rate of CdS by humans which requires a broader appreciation for the acute and chronic toxicity of CdS. We investigated the toxic effects of CdS on cerebellar cell cultures and rat brain. We employed a ‘green synthesis’ biosynthesis process to obtain biocompatible material that can be used in living organisms, such as Viridibacillus arenosi K64. Nanocrystal formation was initiated by adding CdCl2 (1 mM) to the cell cultures. Our in vitro results established that increased concentrations of CdS (0.1 μg/mL) lead to decreased cell viability as assessed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), total antioxidant capacity (TAC), and total oxidant status (TOS). The in vivo studies showed that exposure to CdS (1 mg/kg) glial fibrillary acidic protein (GFAP) and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were increased. Collectively, we describe a model system that addresses the process from the synthesis to the neurotoxicity assessment for CdS both in vitro and in vivo. These data will be beneficial in establishing a more comprehensive pathway for the understanding of quantum dot-induced neurotoxicity. PB - Elsevier T2 - Toxicology Reports T1 - Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies VL - 7 SP - 637 EP - 648 DO - 10.1016/j.toxrep.2020.04.011 ER -
@article{ author = "Varmazyari, Atefeh and Taghizadehghalehjoughi, Ali and Sevim, Cigdem and Baris, Ozlem and Eser, Gizem and Yildirim, Serkan and Hacimuftuoglu, Ahmet and Buha, Aleksandra and Wallace, David R. and Tsatsakis, Aristidis and Aschner, Michael and Mezhuev, Yaroslav", year = "2020", abstract = "Living organisms have an innate ability to regulate the synthesis of inorganic materials, such as bones and teeth in humans. Cadmium sulfide (CdS) can be utilized as a quantum dot that functions as a unique light-emitting semiconductor nanocrystal. The increased use in CdS has led to an increased inhalation and ingestion rate of CdS by humans which requires a broader appreciation for the acute and chronic toxicity of CdS. We investigated the toxic effects of CdS on cerebellar cell cultures and rat brain. We employed a ‘green synthesis’ biosynthesis process to obtain biocompatible material that can be used in living organisms, such as Viridibacillus arenosi K64. Nanocrystal formation was initiated by adding CdCl2 (1 mM) to the cell cultures. Our in vitro results established that increased concentrations of CdS (0.1 μg/mL) lead to decreased cell viability as assessed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), total antioxidant capacity (TAC), and total oxidant status (TOS). The in vivo studies showed that exposure to CdS (1 mg/kg) glial fibrillary acidic protein (GFAP) and 8-hydroxy-2' -deoxyguanosine (8-OHdG) were increased. Collectively, we describe a model system that addresses the process from the synthesis to the neurotoxicity assessment for CdS both in vitro and in vivo. These data will be beneficial in establishing a more comprehensive pathway for the understanding of quantum dot-induced neurotoxicity.", publisher = "Elsevier", journal = "Toxicology Reports", title = "Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies", volume = "7", pages = "637-648", doi = "10.1016/j.toxrep.2020.04.011" }
Varmazyari, A., Taghizadehghalehjoughi, A., Sevim, C., Baris, O., Eser, G., Yildirim, S., Hacimuftuoglu, A., Buha, A., Wallace, D. R., Tsatsakis, A., Aschner, M.,& Mezhuev, Y.. (2020). Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies. in Toxicology Reports Elsevier., 7, 637-648. https://doi.org/10.1016/j.toxrep.2020.04.011
Varmazyari A, Taghizadehghalehjoughi A, Sevim C, Baris O, Eser G, Yildirim S, Hacimuftuoglu A, Buha A, Wallace DR, Tsatsakis A, Aschner M, Mezhuev Y. Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies. in Toxicology Reports. 2020;7:637-648. doi:10.1016/j.toxrep.2020.04.011 .
Varmazyari, Atefeh, Taghizadehghalehjoughi, Ali, Sevim, Cigdem, Baris, Ozlem, Eser, Gizem, Yildirim, Serkan, Hacimuftuoglu, Ahmet, Buha, Aleksandra, Wallace, David R., Tsatsakis, Aristidis, Aschner, Michael, Mezhuev, Yaroslav, "Cadmium sulfide-induced toxicity in the cortex and cerebellum: In vitro and in vivo studies" in Toxicology Reports, 7 (2020):637-648, https://doi.org/10.1016/j.toxrep.2020.04.011 . .