Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
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2021
Authors
Živković, LadaBajić, Vladan
Čabarkapa-Pirković, Andrea
Dekanski, Dragana
Forbes-Hernández, Tamara
Zlatković-Švenda, Mirjana
Perry, George
Spremo-Potparević, Biljana
Article (Published version)
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Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and h...ealthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
Keywords:
DNA damage / ageing / comet assay / neurodegenerative diseaseSource:
Journal of Food Biochemistry, 2021, 45, 4Publisher:
- Wiley
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
- COMET COST action (15132)
DOI: 10.1111/jfbc.13637
ISSN: 0145-8884
WoS: 000615098900001
Scopus: 2-s2.0-85100577124
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PharmacyTY - JOUR AU - Živković, Lada AU - Bajić, Vladan AU - Čabarkapa-Pirković, Andrea AU - Dekanski, Dragana AU - Forbes-Hernández, Tamara AU - Zlatković-Švenda, Mirjana AU - Perry, George AU - Spremo-Potparević, Biljana PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3784 AB - Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD. PB - Wiley T2 - Journal of Food Biochemistry T1 - Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease VL - 45 IS - 4 DO - 10.1111/jfbc.13637 ER -
@article{ author = "Živković, Lada and Bajić, Vladan and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Forbes-Hernández, Tamara and Zlatković-Švenda, Mirjana and Perry, George and Spremo-Potparević, Biljana", year = "2021", abstract = "Increased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.", publisher = "Wiley", journal = "Journal of Food Biochemistry", title = "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease", volume = "45", number = "4", doi = "10.1111/jfbc.13637" }
Živković, L., Bajić, V., Čabarkapa-Pirković, A., Dekanski, D., Forbes-Hernández, T., Zlatković-Švenda, M., Perry, G.,& Spremo-Potparević, B.. (2021). Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry Wiley., 45(4). https://doi.org/10.1111/jfbc.13637
Živković L, Bajić V, Čabarkapa-Pirković A, Dekanski D, Forbes-Hernández T, Zlatković-Švenda M, Perry G, Spremo-Potparević B. Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease. in Journal of Food Biochemistry. 2021;45(4). doi:10.1111/jfbc.13637 .
Živković, Lada, Bajić, Vladan, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Forbes-Hernández, Tamara, Zlatković-Švenda, Mirjana, Perry, George, Spremo-Potparević, Biljana, "Strawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease" in Journal of Food Biochemistry, 45, no. 4 (2021), https://doi.org/10.1111/jfbc.13637 . .