Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage
Само за регистроване кориснике
2022
Аутори
Radovanović, JelenaAntonijević, Biljana
Ćurčić, Marijana
Baralić, Katarina
Kolarević, Stoimir
Bulat, Zorica
Đukić-Ćosić, Danijela
Buha-Đorđević, Aleksandra
Vuković-Gačić, Branka
Javorac, Dragana
Antonijević-Miljaković, Evica
Carević, Momir
Mandinić, Zoran
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Excessive fluoride (F−) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F− subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F−; 3) 25 mg/L F−; 4) 50 mg/L F−; 5) 100 mg/L F−; 6) 150 mg/L F−. Testicles were dissected out and processed for the determination of F− tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups.... The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F−/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F−, this dose-response study is valuable for further research regarding the F− health risk assessment.
Кључне речи:
Bioelements / Oxidative stress / Comet assay / Benchmark methodology / Fluoride / TesticlesИзвор:
Environmental Pollution, 2022, 314Издавач:
- Elsevier Ltd
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Институција/група
PharmacyTY - JOUR AU - Radovanović, Jelena AU - Antonijević, Biljana AU - Ćurčić, Marijana AU - Baralić, Katarina AU - Kolarević, Stoimir AU - Bulat, Zorica AU - Đukić-Ćosić, Danijela AU - Buha-Đorđević, Aleksandra AU - Vuković-Gačić, Branka AU - Javorac, Dragana AU - Antonijević-Miljaković, Evica AU - Carević, Momir AU - Mandinić, Zoran PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4287 AB - Excessive fluoride (F−) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F− subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F−; 3) 25 mg/L F−; 4) 50 mg/L F−; 5) 100 mg/L F−; 6) 150 mg/L F−. Testicles were dissected out and processed for the determination of F− tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F−/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F−, this dose-response study is valuable for further research regarding the F− health risk assessment. PB - Elsevier Ltd T2 - Environmental Pollution T1 - Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage VL - 314 DO - 10.1016/j.envpol.2022.120321 ER -
@article{ author = "Radovanović, Jelena and Antonijević, Biljana and Ćurčić, Marijana and Baralić, Katarina and Kolarević, Stoimir and Bulat, Zorica and Đukić-Ćosić, Danijela and Buha-Đorđević, Aleksandra and Vuković-Gačić, Branka and Javorac, Dragana and Antonijević-Miljaković, Evica and Carević, Momir and Mandinić, Zoran", year = "2022", abstract = "Excessive fluoride (F−) levels in the environment could induce different pathological changes, including comorbidities in reproductive functions. Hence, the aim of the present in vivo study was to explore F− subacute toxicity mechanisms via Benchmark dose (BMD) methodology on rat's testicles. The experiment was conducted on thirty male Wistar rats for 28 days, divided into six groups (n = 5): 1) Control (tap water); 2) 10 mg/L F−; 3) 25 mg/L F−; 4) 50 mg/L F−; 5) 100 mg/L F−; 6) 150 mg/L F−. Testicles were dissected out and processed for the determination of F− tissue concentrations, redox status parameters, essential elements level, and DNA damage. PROASTweb 70.1 software was used for determination of external and internal dose-response relationship. The results confirmed a significant increase in superoxide anion (O2.-), total oxidative status (TOS), copper (Cu), zinc (Zn), iron (Fe), DNA damage levels, and decrease in superoxide dismutase activity (SOD1) and total thiol (SH) groups. The dose-dependent changes were confirmed for SOD1 activity and DNA damage. The most sensitive parameters were SOD1 activity and DNA damage with the lowest BMDLs 0.1 μg F−/kg b. w. Since human and animal populations are daily and frequently unconsciously exposed to F−, this dose-response study is valuable for further research regarding the F− health risk assessment.", publisher = "Elsevier Ltd", journal = "Environmental Pollution", title = "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage", volume = "314", doi = "10.1016/j.envpol.2022.120321" }
Radovanović, J., Antonijević, B., Ćurčić, M., Baralić, K., Kolarević, S., Bulat, Z., Đukić-Ćosić, D., Buha-Đorđević, A., Vuković-Gačić, B., Javorac, D., Antonijević-Miljaković, E., Carević, M.,& Mandinić, Z.. (2022). Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. in Environmental Pollution Elsevier Ltd., 314. https://doi.org/10.1016/j.envpol.2022.120321
Radovanović J, Antonijević B, Ćurčić M, Baralić K, Kolarević S, Bulat Z, Đukić-Ćosić D, Buha-Đorđević A, Vuković-Gačić B, Javorac D, Antonijević-Miljaković E, Carević M, Mandinić Z. Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage. in Environmental Pollution. 2022;314. doi:10.1016/j.envpol.2022.120321 .
Radovanović, Jelena, Antonijević, Biljana, Ćurčić, Marijana, Baralić, Katarina, Kolarević, Stoimir, Bulat, Zorica, Đukić-Ćosić, Danijela, Buha-Đorđević, Aleksandra, Vuković-Gačić, Branka, Javorac, Dragana, Antonijević-Miljaković, Evica, Carević, Momir, Mandinić, Zoran, "Fluoride subacute testicular toxicity in Wistar rats: Benchmark dose analysis for the redox parameters, essential elements and DNA damage" in Environmental Pollution, 314 (2022), https://doi.org/10.1016/j.envpol.2022.120321 . .