Prikaz osnovnih podataka o dokumentu
Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells
dc.creator | Colić, M | |
dc.creator | Stojić-Vukanić, Zorica | |
dc.creator | Pavlović, B | |
dc.creator | Jandrić, Dušan | |
dc.creator | Stefanoska, I | |
dc.date.accessioned | 2019-09-02T10:56:59Z | |
dc.date.available | 2019-09-02T10:56:59Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 0009-9104 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/433 | |
dc.description.abstract | We have studied the effect of mycophenolate mofetil (MMF), a new drug used in prevention of transplant rejection, on differentiation, maturation and allostimulatory activity of human monocyte-derived dendritic cells (MDDC). MDDC were generated in vitro with granulocyte macrophage-colony stimulating factor (GM-CSF) and interleukin (IL)-4 in the presence or absence of MMF. MMF reduced the number of immature MDDC in culture, dose-dependently, by inducing apoptosis and inhibited their stimulatory activity on allogeneic lymphocytes. These changes correlated with down-regulation of costimulatory and adhesion molecules such as CD40, CD54, CD80 and CD86. No differences were observed in mannose receptor (MR)-mediated endocytosis, measured by the uptake of fluorescein isothiocyanate (FITC)-dextran. MDDC differentiated in the presence of MMF showed significantly reduced maturation upon stimulation with lipopolysaccharide, as judged by lower expresson of CD83 and co-stimulatory molecules, lower production of tumour necrosis factor (TNF)-alpha, IL-10, IL-12 and IL-18 as well as lower stimulation of alloreactive T cells including naive CD4(+) CD45RA(+)T cells. In contrast, MDDC matured in the presence of MMF showed a more marked decrease in the FITC-dextran uptake than mature MDDC cultivated without MMF and the phenomenon correlated with down-regulation of the MR expression. These results suggest that MMF impairs differentiation, maturation and function of human MDDC in vitro, which is an additional mechanism of its immunosuppressive effect. | en |
dc.publisher | Blackwell Publishing Ltd, Oxford | |
dc.rights | openAccess | |
dc.source | Clinical and Experimental Immunology | |
dc.subject | alloreactivity | en |
dc.subject | cytokine production | en |
dc.subject | endocytosis | en |
dc.subject | human dendritic cells | en |
dc.subject | mycophenolate mofetil | en |
dc.subject | phenotype | en |
dc.title | Mycophenolate mofetil inhibits differentiation, maturation and allostimulatory function of human monocyte-derived dendritic cells | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Павловић, Б; Стефаноска, И; Јандрић, Душан; Стојић-Вуканић, Зорица; Цолић, М; | |
dc.citation.volume | 134 | |
dc.citation.issue | 1 | |
dc.citation.spage | 63 | |
dc.citation.epage | 69 | |
dc.citation.other | 134(1): 63-69 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000185345200011 | |
dc.identifier.doi | 10.1046/j.1365-2249.2003.02269.x | |
dc.identifier.pmid | 12974756 | |
dc.identifier.scopus | 2-s2.0-19244387277 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs//bitstream/id/1965/431.pdf | |
dc.type.version | publishedVersion |