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Design of experiments and Derringer's desirability function in optimisation and validation of RP-HPLC method for the analysis of enrofloxacin and its impurities
dc.creator | Dokmanović, Jelena | |
dc.creator | Kasagić-Vujanović, Irena | |
dc.creator | Gagić, Žarko | |
dc.creator | Nikolić, Katarina | |
dc.creator | Čarapić, Marija | |
dc.creator | Agbaba, Danica | |
dc.date.accessioned | 2023-03-07T10:38:53Z | |
dc.date.available | 2023-03-07T10:38:53Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 1233-2356 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/4474 | |
dc.description.abstract | Using the Design of Experiments methodology (Response-Surface Methodology and Derringer's Desirability Function), a simple, fast and robust RP-HPLC method was developed for the analysis of enrofloxacin (EFC), its impurity A (fluoroquinolonic acid, FQ) and impurity B (ciprofloxacin, CPX). Gradient elution of samples was performed on a Zorbax Eclipse XDB C18 column (150 × 4.6 mm, 3.5 μm) with a mobile phase consisting of 32 mM phosphate buffer pH 3.5-methanol (0 min-19.6% methanol; 15.5 min-19.6% methanol; 29.5 min-80% methanol; 30 min-19.6% methanol; 35 min-19.6% methanol), delivered at a flow rate of 1.5 mL min-1, wavelength of detection 278 nm (for EFX and CFX) and 265 nm for FQ. A good linear response was achieved in the range 15-35 μg mL-1 (EFX) and LOQ-150% for impurities (CFX and FQ). Other validation parameters were also tested: precision, accuracy, sensitivity and robustness. The developed method was shown to be simple, practical and suitable for the analysis of EFC and its impurities (CPX, FQ) in veterinary drugs. | |
dc.publisher | Akademiai Kiado ZRt. | |
dc.relation | The Ministry for Scientific and Technolog- ical Development, Higher Education and Information Society of the Republic of Srpska for supporting these investigations within the framework of the Project 19.032/961-149/19 | |
dc.rights | openAccess | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | |
dc.source | Acta Chromatographica | |
dc.subject | RP-HPLC | |
dc.subject | Derringer's desirability function | |
dc.subject | design of experiments | |
dc.subject | enrofloxacin | |
dc.subject | optimisation method | |
dc.subject | response surface methodology | |
dc.title | Design of experiments and Derringer's desirability function in optimisation and validation of RP-HPLC method for the analysis of enrofloxacin and its impurities | |
dc.type | article | |
dc.rights.license | BY-NC | |
dc.citation.rank | M23~ | |
dc.identifier.doi | 10.1556/1326.2022.01111 | |
dc.identifier.scopus | 2-s2.0-85148616086 | |
dc.identifier.fulltext | http://farfar.pharmacy.bg.ac.rs/bitstream/id/12035/Design_of_experiments_pub_2023.pdf | |
dc.type.version | publishedVersion |