Optimization of the RP-HPLC method for multicomponent analgetic drug determination.
Апстракт
The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 de...grees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.
Извор:
Bollettino chimico farmaceutico, 2003, 142, 9, 386-389Институција/група
PharmacyTY - JOUR AU - Ivanović, D AU - Medenica, Mirjana AU - Malenović, Anđelija AU - Jančić, Biljana AU - Misljenović, D PY - 2003 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/460 AB - The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs. T2 - Bollettino chimico farmaceutico T1 - Optimization of the RP-HPLC method for multicomponent analgetic drug determination. VL - 142 IS - 9 SP - 386 EP - 389 UR - https://hdl.handle.net/21.15107/rcub_farfar_460 ER -
@article{ author = "Ivanović, D and Medenica, Mirjana and Malenović, Anđelija and Jančić, Biljana and Misljenović, D", year = "2003", abstract = "The optimization of RP-HPLC method defined the simultaneous influence of some important conditions, such as the mobile phase composition, pH of the mobile phase and temperature, on the separation and determination. The RP-HPLC method was done for the determination of paracetamol, caffeine and propyphenazone in a multicomponent pharmaceutical dosage form. The separation factor values define the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The chromatographic system Hewlett Packard 1100 consisted of a HP 1100 pump, HP 1100 UV-VIS detector and HP integrator. Separations were performed on a Beckman Ultrasphere ODS 4.6 x 150 mm, 5 microns particle size column. Samples were introduced through a Rheodyne injector valve with a 20 microL sample loop. UV detection was performed at 265 nm and phenobarottons was used as an internal standard. The optimization was performed within the pH range from 2.5 to 6.0; temperature range from 20 degrees C to 55 degrees C and composition of the mobile phase methanol-water from (30:70 V/V) to (65:35 V/V). The three-D graphs, constructed with sixty-four experimental points, confirmed the optimal conditions for the determination of the investigated analgetic drugs.", journal = "Bollettino chimico farmaceutico", title = "Optimization of the RP-HPLC method for multicomponent analgetic drug determination.", volume = "142", number = "9", pages = "386-389", url = "https://hdl.handle.net/21.15107/rcub_farfar_460" }
Ivanović, D., Medenica, M., Malenović, A., Jančić, B.,& Misljenović, D.. (2003). Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico, 142(9), 386-389. https://hdl.handle.net/21.15107/rcub_farfar_460
Ivanović D, Medenica M, Malenović A, Jančić B, Misljenović D. Optimization of the RP-HPLC method for multicomponent analgetic drug determination.. in Bollettino chimico farmaceutico. 2003;142(9):386-389. https://hdl.handle.net/21.15107/rcub_farfar_460 .
Ivanović, D, Medenica, Mirjana, Malenović, Anđelija, Jančić, Biljana, Misljenović, D, "Optimization of the RP-HPLC method for multicomponent analgetic drug determination." in Bollettino chimico farmaceutico, 142, no. 9 (2003):386-389, https://hdl.handle.net/21.15107/rcub_farfar_460 .