Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle
Само за регистроване кориснике
2004
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Cytogenetic analysis of the X chromosome in phytohaemagglutinin stimulated peripheral blood lymphocytes was evaluated in 12 sporadic Alzheimer disease (AD) patients and in 11 healthy subjects. For chromosome analysis two methods were used: (1) standard analysis of G-banded metaphase chromosomes and; (2) fluorescent in situ hybridization (FISH) for the detection of the X chromosome centromeric region in interphase nuclei. Cytogenetic analysis revealed that the X chromosome expresses premature centromere division (PCD) in AD females in 10.53% of metaphase cells and in 15.22% of interphase nuclei. In AD men the percentages were 3.98 and 6.06%, respectively. X chromosome PCD in the female control group showed a percentage of 7.46% in metaphase cells and 9.35% in interphase nuclei and in male controls the percentages were 2.84% in metaphases and 5.54% in interphase nuclei. The results of FISH analysis showed that PCD could occur much earlier than metaphase of mitosis, i.e. in interphase of ...the cell cycle, immediately after replication. The FISH method can be used for PCD verification in all phases of the cell cycle in various disorders including AD.
Кључне речи:
Alzheimer's disease / fluorescent in situ hybridization / premature centromere division / X chromosome / cell cycleИзвор:
Experimental Gerontology, 2004, 39, 5, 849-854Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/j.exger.2004.01.012
ISSN: 0531-5565
PubMed: 15130680
WoS: 000221633400019
Scopus: 2-s2.0-2342648875
Институција/група
PharmacyTY - JOUR AU - Potparević, Biljana AU - Živković, L AU - Đelić, N AU - Bajić, Vladan PY - 2004 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/477 AB - Cytogenetic analysis of the X chromosome in phytohaemagglutinin stimulated peripheral blood lymphocytes was evaluated in 12 sporadic Alzheimer disease (AD) patients and in 11 healthy subjects. For chromosome analysis two methods were used: (1) standard analysis of G-banded metaphase chromosomes and; (2) fluorescent in situ hybridization (FISH) for the detection of the X chromosome centromeric region in interphase nuclei. Cytogenetic analysis revealed that the X chromosome expresses premature centromere division (PCD) in AD females in 10.53% of metaphase cells and in 15.22% of interphase nuclei. In AD men the percentages were 3.98 and 6.06%, respectively. X chromosome PCD in the female control group showed a percentage of 7.46% in metaphase cells and 9.35% in interphase nuclei and in male controls the percentages were 2.84% in metaphases and 5.54% in interphase nuclei. The results of FISH analysis showed that PCD could occur much earlier than metaphase of mitosis, i.e. in interphase of the cell cycle, immediately after replication. The FISH method can be used for PCD verification in all phases of the cell cycle in various disorders including AD. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Experimental Gerontology T1 - Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle VL - 39 IS - 5 SP - 849 EP - 854 DO - 10.1016/j.exger.2004.01.012 ER -
@article{ author = "Potparević, Biljana and Živković, L and Đelić, N and Bajić, Vladan", year = "2004", abstract = "Cytogenetic analysis of the X chromosome in phytohaemagglutinin stimulated peripheral blood lymphocytes was evaluated in 12 sporadic Alzheimer disease (AD) patients and in 11 healthy subjects. For chromosome analysis two methods were used: (1) standard analysis of G-banded metaphase chromosomes and; (2) fluorescent in situ hybridization (FISH) for the detection of the X chromosome centromeric region in interphase nuclei. Cytogenetic analysis revealed that the X chromosome expresses premature centromere division (PCD) in AD females in 10.53% of metaphase cells and in 15.22% of interphase nuclei. In AD men the percentages were 3.98 and 6.06%, respectively. X chromosome PCD in the female control group showed a percentage of 7.46% in metaphase cells and 9.35% in interphase nuclei and in male controls the percentages were 2.84% in metaphases and 5.54% in interphase nuclei. The results of FISH analysis showed that PCD could occur much earlier than metaphase of mitosis, i.e. in interphase of the cell cycle, immediately after replication. The FISH method can be used for PCD verification in all phases of the cell cycle in various disorders including AD.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Experimental Gerontology", title = "Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle", volume = "39", number = "5", pages = "849-854", doi = "10.1016/j.exger.2004.01.012" }
Potparević, B., Živković, L., Đelić, N.,& Bajić, V.. (2004). Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle. in Experimental Gerontology Pergamon-Elsevier Science Ltd, Oxford., 39(5), 849-854. https://doi.org/10.1016/j.exger.2004.01.012
Potparević B, Živković L, Đelić N, Bajić V. Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle. in Experimental Gerontology. 2004;39(5):849-854. doi:10.1016/j.exger.2004.01.012 .
Potparević, Biljana, Živković, L, Đelić, N, Bajić, Vladan, "Analysis of premature centromere division (PCD) of the X chromosome in Alzheimer patients through the cell cycle" in Experimental Gerontology, 39, no. 5 (2004):849-854, https://doi.org/10.1016/j.exger.2004.01.012 . .