Comparison of HPTLC and HPLC for determination of econazole nitrate in topical dosage forms
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HPTLC and HPLC methods have been established for separation and quantitative determination of econazole nitrate. HPTLC was performed on silica gel plates with n-butyl acetate-carbon tetrachloride-methanol-diethylamine, 3 + 6 + 2.5 + 0.5 (v/v), as mobile phase. Chromatographic zones, or spots, of econazole base and nitrate were used to quantify econazole nitrate. HPLC was performed on amino and C-8 columns with acetonitrile-water, 30 + 70 (v/v), pH 2.5 (adjusted with phosphoric acid), as a mobile phase. The chromatograms were characterized by single peaks of econazole (amino column) or nitrate (C-8 column). The methods were successfully used for determination of econazole nitrate in spray solution and vaginal pessaries.
Keywords:econazole nitrate / HPTLC / HPLC / pharmaceutical formulations
Source:Journal of Planar Chromatography - Modern TLC, 2004, 17, 2, 109-112
- Research Inst Medicinal Plants, Budakalasz