Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits
Апстракт
Composition of dichloromethane and methanol extracts from roots and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (collected in Sićevo Gorge, Serbia in 2020) was investigated. Extracts were obtained by bimaceration, firstly with dichloromethane, then with methanol; solvents were removed under reduced pressure. GC-FID-MS analysis, conducted after saponification and methylation of samples, showed that palmitic, oleic and linoleic (14.2-51.8%) were dominant fatty acids in root dichloromethane extract, and oleic, linoleic and petroselinic in oily supernatant of fruit dichloromethane extract (10.2-49.9%); in both samples 16 fatty acids were identified. Dominant phytosterols, investigated by GC-FID-MS after unsaponifiable fractions silanization, were β-sitosterol (48.2 and 25.1%) and stigmasterol (14.9 and 12.8%). LC-DAD-MS analysis of dichloromethane and methanol extracts revealed presence of 7-11 furanocoumarins, including oxypeucedanin and imperatorin (up to 105.9 and 49.2 mg/g i...n fruit dichloromethane extract crystalline precipitate), followed by smaller amounts of oxypeucedanin hydrate, isoimperatorin, heraclenol, heraclenin and/or xanthotoxin. All extracts were rich in one 2',3'-dihydrofuranocoumarin derivative. Previously, such derivative, prantschimgin, as well as imperatorin and isoimperatorin were isolated from this plant and their in vitro anti-inflammatory activity was demonstrated [1]. In current investigation, in root methanol extract, chlorogenic (31.6 mg/g), two dicaffeoylquinic (12.8 and 48.1 mg/g), and smaller amounts of one caffeoylquinic and one feruloylquinic acids were also determined. Furthermore, low quantities of chlorogenic and one dicaffeoylquinic acid and two quercetin-type flavonoids were detected in fruit methanol extract, and one methylluteolin-type flavonoid in both methanol extracts.
Извор:
5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 2023, 269-269Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Напомена:
- 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023, Sts. Constantine & Helena, Bulgaria, 30th May to 2nd June 2023
- Saopštenje sa međunarodnog skupa štampano u izvodu
Институција/група
PharmacyTY - CONF AU - Ušjak, Ljuboš AU - Niketić, Marjan AU - Petrović, Silvana PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4900 AB - Composition of dichloromethane and methanol extracts from roots and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (collected in Sićevo Gorge, Serbia in 2020) was investigated. Extracts were obtained by bimaceration, firstly with dichloromethane, then with methanol; solvents were removed under reduced pressure. GC-FID-MS analysis, conducted after saponification and methylation of samples, showed that palmitic, oleic and linoleic (14.2-51.8%) were dominant fatty acids in root dichloromethane extract, and oleic, linoleic and petroselinic in oily supernatant of fruit dichloromethane extract (10.2-49.9%); in both samples 16 fatty acids were identified. Dominant phytosterols, investigated by GC-FID-MS after unsaponifiable fractions silanization, were β-sitosterol (48.2 and 25.1%) and stigmasterol (14.9 and 12.8%). LC-DAD-MS analysis of dichloromethane and methanol extracts revealed presence of 7-11 furanocoumarins, including oxypeucedanin and imperatorin (up to 105.9 and 49.2 mg/g in fruit dichloromethane extract crystalline precipitate), followed by smaller amounts of oxypeucedanin hydrate, isoimperatorin, heraclenol, heraclenin and/or xanthotoxin. All extracts were rich in one 2',3'-dihydrofuranocoumarin derivative. Previously, such derivative, prantschimgin, as well as imperatorin and isoimperatorin were isolated from this plant and their in vitro anti-inflammatory activity was demonstrated [1]. In current investigation, in root methanol extract, chlorogenic (31.6 mg/g), two dicaffeoylquinic (12.8 and 48.1 mg/g), and smaller amounts of one caffeoylquinic and one feruloylquinic acids were also determined. Furthermore, low quantities of chlorogenic and one dicaffeoylquinic acid and two quercetin-type flavonoids were detected in fruit methanol extract, and one methylluteolin-type flavonoid in both methanol extracts. C3 - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts T1 - Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits SP - 269 EP - 269 UR - https://hdl.handle.net/21.15107/rcub_farfar_4900 ER -
@conference{ author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana", year = "2023", abstract = "Composition of dichloromethane and methanol extracts from roots and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (collected in Sićevo Gorge, Serbia in 2020) was investigated. Extracts were obtained by bimaceration, firstly with dichloromethane, then with methanol; solvents were removed under reduced pressure. GC-FID-MS analysis, conducted after saponification and methylation of samples, showed that palmitic, oleic and linoleic (14.2-51.8%) were dominant fatty acids in root dichloromethane extract, and oleic, linoleic and petroselinic in oily supernatant of fruit dichloromethane extract (10.2-49.9%); in both samples 16 fatty acids were identified. Dominant phytosterols, investigated by GC-FID-MS after unsaponifiable fractions silanization, were β-sitosterol (48.2 and 25.1%) and stigmasterol (14.9 and 12.8%). LC-DAD-MS analysis of dichloromethane and methanol extracts revealed presence of 7-11 furanocoumarins, including oxypeucedanin and imperatorin (up to 105.9 and 49.2 mg/g in fruit dichloromethane extract crystalline precipitate), followed by smaller amounts of oxypeucedanin hydrate, isoimperatorin, heraclenol, heraclenin and/or xanthotoxin. All extracts were rich in one 2',3'-dihydrofuranocoumarin derivative. Previously, such derivative, prantschimgin, as well as imperatorin and isoimperatorin were isolated from this plant and their in vitro anti-inflammatory activity was demonstrated [1]. In current investigation, in root methanol extract, chlorogenic (31.6 mg/g), two dicaffeoylquinic (12.8 and 48.1 mg/g), and smaller amounts of one caffeoylquinic and one feruloylquinic acids were also determined. Furthermore, low quantities of chlorogenic and one dicaffeoylquinic acid and two quercetin-type flavonoids were detected in fruit methanol extract, and one methylluteolin-type flavonoid in both methanol extracts.", journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts", title = "Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits", pages = "269-269", url = "https://hdl.handle.net/21.15107/rcub_farfar_4900" }
Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 269-269. https://hdl.handle.net/21.15107/rcub_farfar_4900
Ušjak L, Niketić M, Petrović S. Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:269-269. https://hdl.handle.net/21.15107/rcub_farfar_4900 .
Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Fatty acids, phytosterols, furanocoumarins and polyphenols of Prangos trifida s.l. (Apiaceae) roots and fruits" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):269-269, https://hdl.handle.net/21.15107/rcub_farfar_4900 .