Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract
Апстракт
The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists.
In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics conte...nt at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).
Кључне речи:
Stachys recta subsp. recta / phenolics / LC-MS / DPPHИзвор:
5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 2023, 196-196Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Напомена:
- 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023, Sts. Constantine & Helena, Bulgaria, 30th May to 2nd June 2023
- Saopštenje sa međunarodnog skupa štampano u izvodu
Институција/група
PharmacyTY - CONF AU - Kukić-Marković, Jelena AU - Milutinović, Violeta AU - Niketić, Marjan AU - Petrović, Silvana PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4988 AB - The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists. In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively). C3 - 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts T1 - Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract SP - 196 EP - 196 UR - https://hdl.handle.net/21.15107/rcub_farfar_4988 ER -
@conference{ author = "Kukić-Marković, Jelena and Milutinović, Violeta and Niketić, Marjan and Petrović, Silvana", year = "2023", abstract = "The highest richness of taxa within genus Stachys was recorded in the wider area of the Mediterranean, where the group S. recta stands out in terms of taxonomic complexity. The center of diversity of this group is on the Balkan Peninsula. Its representatives are still insufficiently defined in taxonomic and horological terms, and their traditional approach includes about fifteen taxa, mostly at the infraspecific level. Despite some radical approaches that were presented at the end of the last century [1], the traditional taxonomic treatment is still prevailed in the relevant global lists. In this work antiradical potential and phenolic constituents of S. recta L. subsp. recta were investigated. Plant material, collected in Central Serbia (vicinity of Prokuplje), was air-dried, powdered, and then extracted by bimaceration with chloroform and methanol. Obtained dry methanol extract exhibited strong, concentration-dependant anti-DPPH activity (SC50=8.23 μg/mL), with total phenolics content at 0.27 mg GAE/mg of dry extract. In TLC-DPPH test observed yellow zones indicated presence of few compounds which strongly neutralized purple DPPH radical. LC-MS analysis revealed presence of 24 phenolic compounds, belonging to three classes of constituents: phenolic acids, phenylethanol glycosides and flavonoids. Chlorogenic acid (2.63%) was the only phenolic acid, while acteoside was the most dominant phenylethanoid (24.03%). Flavonoid fraction was composed almost entirely of 8-hydroxyflavones, derivatives of hypolaetine and isoscutellareine (53.47%). The most prominent flavonoids in investigated extract were 7-O-[6'''-acetylallosyl(1®2)]-glucopyranosides of 4'-O-methylhypolaetine and hypolaetine (15.92 and 13.01%, respectively).", journal = "5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts", title = "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract", pages = "196-196", url = "https://hdl.handle.net/21.15107/rcub_farfar_4988" }
Kukić-Marković, J., Milutinović, V., Niketić, M.,& Petrović, S.. (2023). Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts, 196-196. https://hdl.handle.net/21.15107/rcub_farfar_4988
Kukić-Marković J, Milutinović V, Niketić M, Petrović S. Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract. in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts. 2023;:196-196. https://hdl.handle.net/21.15107/rcub_farfar_4988 .
Kukić-Marković, Jelena, Milutinović, Violeta, Niketić, Marjan, Petrović, Silvana, "Antiradical activity and phenolic constituents of Stachys recta L. subsp. recta herb methanol extract" in 5th International Conference on Natural Products Utilization: from Plants to Pharmacy Shelf - ICNPU-2023 Book of Abstracts (2023):196-196, https://hdl.handle.net/21.15107/rcub_farfar_4988 .