Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software
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Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for analysts. Such a case regarding the analysis of bupropion hydrochloride and its 5 impurities in a tablet formulation was used as a model. A baseline correction technique for liquid chromatography coupled with diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm was used as implemented in the “baseline” package, with parameters lambda and p set to 4 and 0.05, respectively. Peak deconvolution and subsequent integration and area quantification were accomplished through Fytik software. Chromatographic data from the validation procedure were utilized to demonstrate the feasibility of the suggested method and whether this correction affects the outcome of the validation study. Finally, a robustness study was carried out in order to shed light on the factors that have a more significant influence on the baseline correction, showing the reliability of t...his procedure through random changes in its parameters.
Кључне речи:
asymmetry least squares algorithm / baseline correction / data processing / experimental design / peak integration / robustness testingИзвор:
Analytica, 2023, 4, 1, 45-53Издавач:
- MDPI
Институција/група
PharmacyTY - JOUR AU - Gkountanas, Kostas AU - Dagla, Ioanna AU - Gikas, Evangelos AU - Malenović, Anđelija AU - Dotsikas, Yannis PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4998 AB - Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for analysts. Such a case regarding the analysis of bupropion hydrochloride and its 5 impurities in a tablet formulation was used as a model. A baseline correction technique for liquid chromatography coupled with diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm was used as implemented in the “baseline” package, with parameters lambda and p set to 4 and 0.05, respectively. Peak deconvolution and subsequent integration and area quantification were accomplished through Fytik software. Chromatographic data from the validation procedure were utilized to demonstrate the feasibility of the suggested method and whether this correction affects the outcome of the validation study. Finally, a robustness study was carried out in order to shed light on the factors that have a more significant influence on the baseline correction, showing the reliability of this procedure through random changes in its parameters. PB - MDPI T2 - Analytica T1 - Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software VL - 4 IS - 1 SP - 45 EP - 53 DO - 10.3390/analytica4010005 ER -
@article{ author = "Gkountanas, Kostas and Dagla, Ioanna and Gikas, Evangelos and Malenović, Anđelija and Dotsikas, Yannis", year = "2023", abstract = "Chromatograms with overlapping peaks and a baseline rise or upset constitute a great challenge for analysts. Such a case regarding the analysis of bupropion hydrochloride and its 5 impurities in a tablet formulation was used as a model. A baseline correction technique for liquid chromatography coupled with diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm was used as implemented in the “baseline” package, with parameters lambda and p set to 4 and 0.05, respectively. Peak deconvolution and subsequent integration and area quantification were accomplished through Fytik software. Chromatographic data from the validation procedure were utilized to demonstrate the feasibility of the suggested method and whether this correction affects the outcome of the validation study. Finally, a robustness study was carried out in order to shed light on the factors that have a more significant influence on the baseline correction, showing the reliability of this procedure through random changes in its parameters.", publisher = "MDPI", journal = "Analytica", title = "Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software", volume = "4", number = "1", pages = "45-53", doi = "10.3390/analytica4010005" }
Gkountanas, K., Dagla, I., Gikas, E., Malenović, A.,& Dotsikas, Y.. (2023). Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software. in Analytica MDPI., 4(1), 45-53. https://doi.org/10.3390/analytica4010005
Gkountanas K, Dagla I, Gikas E, Malenović A, Dotsikas Y. Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software. in Analytica. 2023;4(1):45-53. doi:10.3390/analytica4010005 .
Gkountanas, Kostas, Dagla, Ioanna, Gikas, Evangelos, Malenović, Anđelija, Dotsikas, Yannis, "Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software" in Analytica, 4, no. 1 (2023):45-53, https://doi.org/10.3390/analytica4010005 . .