Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations
Samo za registrovane korisnike
1989
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens.
Ključne reči:
Conjugated estrogens / fluorodensitometric determination / raw material and pharmaceutical preparation.Izvor:
Journal of Pharmaceutical and Biomedical Analysis, 1989, 7, 12, 1657-1662Izdavač:
- Elsevier
Institucija/grupa
PharmacyTY - JOUR AU - Novaković, J AU - Agbaba, Danica AU - Stakić, D.Ž PY - 1989 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/49 AB - A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens. PB - Elsevier T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations VL - 7 IS - 12 SP - 1657 EP - 1662 DO - 10.1016/0731-7085(89)80179-7 ER -
@article{ author = "Novaković, J and Agbaba, Danica and Stakić, D.Ž", year = "1989", abstract = "A simple, rapid and reproducible fluorodensitometric method for the determination of conjugated estrogens has been developed. The proposed procedure includes the following steps: extraction, hydrolysis of sodium sulphate esters of estrone, equilin, equilenin and their 17-α-hydroxy derivatives, separation of the liberated 3-phenolic steroids and in situ measurement of fluorescence. The fluorescence emission was measured after spraying the spots of estrone and estradiol with 2,4-dinitrophenylhydrazine in sulphuric acid-ethanol medium and equilin and 17-α-dihydroequilin with phosphoric acid and sodium hydroxide solution, respectively. Equilenin and 17-α-dihydroequilenin were determined by measuring the native fluorescence. The method applied to the determination of raw material and tablets provided results which agreed well with the stated content and the requirements of USP XXI for conjugated estrogens.", publisher = "Elsevier", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations", volume = "7", number = "12", pages = "1657-1662", doi = "10.1016/0731-7085(89)80179-7" }
Novaković, J., Agbaba, D.,& Stakić, D.Ž. (1989). Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis Elsevier., 7(12), 1657-1662. https://doi.org/10.1016/0731-7085(89)80179-7
Novaković J, Agbaba D, Stakić D. Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis. 1989;7(12):1657-1662. doi:10.1016/0731-7085(89)80179-7 .
Novaković, J, Agbaba, Danica, Stakić, D.Ž, "Fluorodensitometric determination of conjugated estrogens in raw material and in pharmaceutical preparations" in Journal of Pharmaceutical and Biomedical Analysis, 7, no. 12 (1989):1657-1662, https://doi.org/10.1016/0731-7085(89)80179-7 . .