Biomimetic characteristics of dual TLC retention mechanism
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Most biomimetic chromatography measurements provide information on the ability of
drugs to pass through cell membranes, their interaction with protein-based structures and
distribution properties. The biomimetic properties of thin-layer chromatography (TLC)
conditions have not been investigated so far. In our previous research, the presence of dual
retention mechanisms for selected imidazoline and serotonin receptor ligands was
confirmed under TLC conditions on C-18, diol, and a silica-based phases. The mobile phase
was amixture of ACN and water with 20 mM ammoniumacetate and 0.1 volume %of acetic
acid [1]. In this research, the average retention parameters were determined by using the
integration procedure [2]. The parameter RMH is the average retention in the hydrophilicdominated
(HILIC), while RMR is the average retention in the region of reversed-phase
interactions (RP). The parameter RMA corresponds to the average retention within the
overall HILIC/RP region. The lipoph...ilicity successfully correlates with the C-18 and silica
based behaviour. For plasma protein binding affinity, the best correlations were found
within C-18 and silica-based systems (r > 0.70). There is also a correlation of average silica
gel and C-18 mechanism of interaction with the volume of distribution (r > 0.73), and the
intestinal absorption (r > 0.70). The retention behaviour on the diol phase showed a good
correlation with the P-gp inhibitor activity (r = 0.80). TLC systems that provide dual
retention mechanisms can be successfully used in the rapid biomimetic profiling of
serotonin and imidazoline receptor ligands in the first steps of drug discovery.
Кључне речи:
biomimetic chromatography / thin-layer chromatography TLC / HILICИзвор:
10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6, 2023, 48-48Издавач:
- International Association of Physical Chemists
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Напомена:
- 10th IAPC Meeting, Book of Abstracts
Институција/група
PharmacyTY - CONF AU - Obradović, Darija AU - Komsta, Lukasz AU - Popović-Nikolić, Marija AU - Milutinović, Jovana AU - Lazović, Saša PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5002 AB - Most biomimetic chromatography measurements provide information on the ability of drugs to pass through cell membranes, their interaction with protein-based structures and distribution properties. The biomimetic properties of thin-layer chromatography (TLC) conditions have not been investigated so far. In our previous research, the presence of dual retention mechanisms for selected imidazoline and serotonin receptor ligands was confirmed under TLC conditions on C-18, diol, and a silica-based phases. The mobile phase was amixture of ACN and water with 20 mM ammoniumacetate and 0.1 volume %of acetic acid [1]. In this research, the average retention parameters were determined by using the integration procedure [2]. The parameter RMH is the average retention in the hydrophilicdominated (HILIC), while RMR is the average retention in the region of reversed-phase interactions (RP). The parameter RMA corresponds to the average retention within the overall HILIC/RP region. The lipophilicity successfully correlates with the C-18 and silica based behaviour. For plasma protein binding affinity, the best correlations were found within C-18 and silica-based systems (r > 0.70). There is also a correlation of average silica gel and C-18 mechanism of interaction with the volume of distribution (r > 0.73), and the intestinal absorption (r > 0.70). The retention behaviour on the diol phase showed a good correlation with the P-gp inhibitor activity (r = 0.80). TLC systems that provide dual retention mechanisms can be successfully used in the rapid biomimetic profiling of serotonin and imidazoline receptor ligands in the first steps of drug discovery. PB - International Association of Physical Chemists C3 - 10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 T1 - Biomimetic characteristics of dual TLC retention mechanism SP - 48 EP - 48 UR - https://hdl.handle.net/21.15107/rcub_farfar_5002 ER -
@conference{ author = "Obradović, Darija and Komsta, Lukasz and Popović-Nikolić, Marija and Milutinović, Jovana and Lazović, Saša", year = "2023", abstract = "Most biomimetic chromatography measurements provide information on the ability of drugs to pass through cell membranes, their interaction with protein-based structures and distribution properties. The biomimetic properties of thin-layer chromatography (TLC) conditions have not been investigated so far. In our previous research, the presence of dual retention mechanisms for selected imidazoline and serotonin receptor ligands was confirmed under TLC conditions on C-18, diol, and a silica-based phases. The mobile phase was amixture of ACN and water with 20 mM ammoniumacetate and 0.1 volume %of acetic acid [1]. In this research, the average retention parameters were determined by using the integration procedure [2]. The parameter RMH is the average retention in the hydrophilicdominated (HILIC), while RMR is the average retention in the region of reversed-phase interactions (RP). The parameter RMA corresponds to the average retention within the overall HILIC/RP region. The lipophilicity successfully correlates with the C-18 and silica based behaviour. For plasma protein binding affinity, the best correlations were found within C-18 and silica-based systems (r > 0.70). There is also a correlation of average silica gel and C-18 mechanism of interaction with the volume of distribution (r > 0.73), and the intestinal absorption (r > 0.70). The retention behaviour on the diol phase showed a good correlation with the P-gp inhibitor activity (r = 0.80). TLC systems that provide dual retention mechanisms can be successfully used in the rapid biomimetic profiling of serotonin and imidazoline receptor ligands in the first steps of drug discovery.", publisher = "International Association of Physical Chemists", journal = "10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6", title = "Biomimetic characteristics of dual TLC retention mechanism", pages = "48-48", url = "https://hdl.handle.net/21.15107/rcub_farfar_5002" }
Obradović, D., Komsta, L., Popović-Nikolić, M., Milutinović, J.,& Lazović, S.. (2023). Biomimetic characteristics of dual TLC retention mechanism. in 10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 International Association of Physical Chemists., 48-48. https://hdl.handle.net/21.15107/rcub_farfar_5002
Obradović D, Komsta L, Popović-Nikolić M, Milutinović J, Lazović S. Biomimetic characteristics of dual TLC retention mechanism. in 10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6. 2023;:48-48. https://hdl.handle.net/21.15107/rcub_farfar_5002 .
Obradović, Darija, Komsta, Lukasz, Popović-Nikolić, Marija, Milutinović, Jovana, Lazović, Saša, "Biomimetic characteristics of dual TLC retention mechanism" in 10th IAPC Meeting, Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 (2023):48-48, https://hdl.handle.net/21.15107/rcub_farfar_5002 .