Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent
Само за регистроване кориснике
2004
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol con...tent were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0.
Кључне речи:
simvastatin / impurities / LC / microemulsionИзвор:
Journal of Separation Science, 2004, 27, 13, 1087-1092Издавач:
- Wiley-VCH Verlag GMBH, Weinheim
DOI: 10.1002/jssc.200401748
ISSN: 1615-9306
PubMed: 15495410
WoS: 000224188000004
Scopus: 2-s2.0-4844223516
Институција/група
PharmacyTY - JOUR AU - Malenović, Anđelija AU - Ivanović, D AU - Medenica, Mirjana AU - Jančić, Biljana AU - Marković, S PY - 2004 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/505 AB - A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol content were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0. PB - Wiley-VCH Verlag GMBH, Weinheim T2 - Journal of Separation Science T1 - Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent VL - 27 IS - 13 SP - 1087 EP - 1092 DO - 10.1002/jssc.200401748 ER -
@article{ author = "Malenović, Anđelija and Ivanović, D and Medenica, Mirjana and Jančić, Biljana and Marković, S", year = "2004", abstract = "A novel and unique approach was used for retention modelling in the separation of simvastatin and six impurities by liquid chromatographic using a microemulsion as mobile phase. A microemulsion is a modification of a micellar system where a lipophilic organic solvent is dissolved in the micelles; for that reason, microemulsions are usually treated as solvent-modified micellar solutions. When microemulsions are used as eluents in HPLC separations, solutes partition between the charged oil droplets and the aqueous buffer phase. The complexity of the composition of the microemulsion permits extensive manipulations to be made during method development in order to achieve acceptable resolution of such a complex mixture of substances. In order to avoid a laborious "trial and error" procedure, a 2(3) full factorial design was applied for choosing an optimal microemulsion composition to obtain good separation in a reasonable run time. Organic solvent, sodium dodecyl sulphate, and n-butanol content were varied within defined experimental domain. Optimal conditions for the separation of simvastatin and its six impurities were obtained using an X Terra(TM) 50 x 4.6 mm, 3.5 mum particle size column at 30degreesC. The mobile phase consisted of 0.9% w/w of diisopropyl ether, 2.2% w/w of sodium dodecylsulphate (SDS), 7.0% w/w of co-surfactant such as n-butanol, and 89.9% w/w of aqueous 25 mM disodium phosphate pH 7.0.", publisher = "Wiley-VCH Verlag GMBH, Weinheim", journal = "Journal of Separation Science", title = "Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent", volume = "27", number = "13", pages = "1087-1092", doi = "10.1002/jssc.200401748" }
Malenović, A., Ivanović, D., Medenica, M., Jančić, B.,& Marković, S.. (2004). Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent. in Journal of Separation Science Wiley-VCH Verlag GMBH, Weinheim., 27(13), 1087-1092. https://doi.org/10.1002/jssc.200401748
Malenović A, Ivanović D, Medenica M, Jančić B, Marković S. Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent. in Journal of Separation Science. 2004;27(13):1087-1092. doi:10.1002/jssc.200401748 .
Malenović, Anđelija, Ivanović, D, Medenica, Mirjana, Jančić, Biljana, Marković, S, "Retention modelling in liquid chromatographic separation of simvastatin and six impurities using a microemulsion as eluent" in Journal of Separation Science, 27, no. 13 (2004):1087-1092, https://doi.org/10.1002/jssc.200401748 . .