Epigenetic Aspects of a New Probiotic Concept—A Pilot Study
Аутори
Okuka, NinaSchuh, Verena
Krammer, Ulrike
Polovina, Snežana
Šumarac-Dumanović, Mirjana
Milinković, Neda
Veličković, Ksenija
Đorđević, Brižita
Haslberger, Alexander
Ivanović, Nevena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031)... showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.
Кључне речи:
obesity / epigenetic markers / Lactobacillus plantarum 299v / miRNAs / mRNA / policosanols / probiotics / Saccharomyces boulardii var. cerevisiae / SIRT1Извор:
Life, 2023, 13, 9Издавач:
- MDPI
Финансирање / пројекти:
- bilateral project with Austria, Grant No.: 451-03- 02141/2017-09/35
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.3390/life13091912
ISSN: 2075-1729
PubMed: 37763315
Scopus: 2-s2.0-85172234746
Институција/група
PharmacyTY - JOUR AU - Okuka, Nina AU - Schuh, Verena AU - Krammer, Ulrike AU - Polovina, Snežana AU - Šumarac-Dumanović, Mirjana AU - Milinković, Neda AU - Veličković, Ksenija AU - Đorđević, Brižita AU - Haslberger, Alexander AU - Ivanović, Nevena PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5054 AB - Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control. PB - MDPI T2 - Life T1 - Epigenetic Aspects of a New Probiotic Concept—A Pilot Study VL - 13 IS - 9 DO - 10.3390/life13091912 ER -
@article{ author = "Okuka, Nina and Schuh, Verena and Krammer, Ulrike and Polovina, Snežana and Šumarac-Dumanović, Mirjana and Milinković, Neda and Veličković, Ksenija and Đorđević, Brižita and Haslberger, Alexander and Ivanović, Nevena", year = "2023", abstract = "Several studies report the important role of an altered gut microbiota in the development of obesity, highlighting the potential use of probiotics in the treatment of obesity. The aim of this study is to investigate the effect of a novel probiotic approach on the expression of specific miRNAs and mRNAs associated with obesity in combination with the hypocholesterolemic octacosanol. Twenty overweight/obese women participated in a randomized, placebo-controlled, double-blind study and were randomly divided into two groups: the intervention group (daily one capsule containing Lactobacillus plantarum 299v (DSM9843), Saccharomyces cerevisiae var. boulardii, and 40 mg octacosanol; N = 12) and the placebo group (N = 8). Changes in lipid parameters and expression of miRNAs and mRNAs were assessed before (T0) and after the 12-week intervention (T1). After the intervention, the expression of miR-155-5p (9.38 ± 0.85 vs. 8.38 ± 1.06, p = 0.05) and miR-24-3p (3.42 ± 0.38 vs. 2.71 ± 0.97, p = 0.031) showed significant decreases in the intervention group when compared to the control group. At T1, the expression of miR-155-5p (8.69 ± 1.31 vs. 9.3 ± 0.85, p = 0.04), miR-125b-5p (5.41 ± 1.18 vs. 5.99 ± 1.36, p = 0.049), and TNF-α (10.24 ± 1.66 vs. 11.36 ± 1.12, p = 0.009) were significantly decreased in the intervention group. No changes in lipids and anthropometric parameters were observed. The novel probiotic approach had a positive effect on regulating the expression of certain miRNAs and mRNAs important for regulating inflammation and adipogenesis, which are essential for obesity onset and control.", publisher = "MDPI", journal = "Life", title = "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study", volume = "13", number = "9", doi = "10.3390/life13091912" }
Okuka, N., Schuh, V., Krammer, U., Polovina, S., Šumarac-Dumanović, M., Milinković, N., Veličković, K., Đorđević, B., Haslberger, A.,& Ivanović, N.. (2023). Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life MDPI., 13(9). https://doi.org/10.3390/life13091912
Okuka N, Schuh V, Krammer U, Polovina S, Šumarac-Dumanović M, Milinković N, Veličković K, Đorđević B, Haslberger A, Ivanović N. Epigenetic Aspects of a New Probiotic Concept—A Pilot Study. in Life. 2023;13(9). doi:10.3390/life13091912 .
Okuka, Nina, Schuh, Verena, Krammer, Ulrike, Polovina, Snežana, Šumarac-Dumanović, Mirjana, Milinković, Neda, Veličković, Ksenija, Đorđević, Brižita, Haslberger, Alexander, Ivanović, Nevena, "Epigenetic Aspects of a New Probiotic Concept—A Pilot Study" in Life, 13, no. 9 (2023), https://doi.org/10.3390/life13091912 . .