Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids
Аутори
Subošić, BrankoKotur-Stevuljević, Jelena
Brborić, Jasmina
Janković, Tamara
Milenković, Marina
Ivković, Branka
Kostadinović, Jelena
Savić, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven ...acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen.
Кључне речи:
Inflammation / oxidative stress / anti-inflammatory compounds / oxy scoreИзвор:
Pakistan Journal of Pharmaceutical Sciences, 2023, 36, 5, 1367-1374Издавач:
- Pakistan Journal of Pharmaceutical Sciences
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.36721/PJPS.2023.36.5.REG.1367-1374.1
ISSN: 1011-601X
PubMed: 37869911
Scopus: 2-s2.0-85173646115
Институција/група
PharmacyTY - JOUR AU - Subošić, Branko AU - Kotur-Stevuljević, Jelena AU - Brborić, Jasmina AU - Janković, Tamara AU - Milenković, Marina AU - Ivković, Branka AU - Kostadinović, Jelena AU - Savić, Jelena PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5146 AB - The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen. PB - Pakistan Journal of Pharmaceutical Sciences T2 - Pakistan Journal of Pharmaceutical Sciences T1 - Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids VL - 36 IS - 5 SP - 1367 EP - 1374 DO - 10.36721/PJPS.2023.36.5.REG.1367-1374.1 ER -
@article{ author = "Subošić, Branko and Kotur-Stevuljević, Jelena and Brborić, Jasmina and Janković, Tamara and Milenković, Marina and Ivković, Branka and Kostadinović, Jelena and Savić, Jelena", year = "2023", abstract = "The interplay between oxidative stress and inflammation is implicated in many chronic diseases including Alzheimer`s disease, cardiovascular diseases, diabetes and cancer. Thirteen β-hydroxy-β-arylalkanoic acids were previously synthesized and evaluated for their anti-inflammatory activity. The aim of this study was to asses ex vivo antioxidant activity of synthesized acids, as well as ibuprofen and to identify the compounds with the most promising results for further investigation on their capacity to counteract in vivo oxidative stress triggered by inflammation. The antioxidant potential of tested compounds was evaluated by determining the concentrations of total antioxidative status, total oxidative status, prooxidant antioxidant balance and the total sulfhydryl groups. Z score statistics were used to calculate the summary scores for antioxidative activity, prooxidative activity and oxy score. The tested compounds and ibuprofen demonstrated mild prooxidative activity ex vivo. Seven acids with substituents on one benzene ring exhibited better results than ibuprofen and were selected for in vivo testing. In vivo results demonstrated better antioxidant protection compared to ex vivo results. Compound g which contains nitro group on the benzene ring demonstrated the lowest oxy score, and four compounds exhibited better results than ibuprofen.", publisher = "Pakistan Journal of Pharmaceutical Sciences", journal = "Pakistan Journal of Pharmaceutical Sciences", title = "Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids", volume = "36", number = "5", pages = "1367-1374", doi = "10.36721/PJPS.2023.36.5.REG.1367-1374.1" }
Subošić, B., Kotur-Stevuljević, J., Brborić, J., Janković, T., Milenković, M., Ivković, B., Kostadinović, J.,& Savić, J.. (2023). Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids. in Pakistan Journal of Pharmaceutical Sciences Pakistan Journal of Pharmaceutical Sciences., 36(5), 1367-1374. https://doi.org/10.36721/PJPS.2023.36.5.REG.1367-1374.1
Subošić B, Kotur-Stevuljević J, Brborić J, Janković T, Milenković M, Ivković B, Kostadinović J, Savić J. Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids. in Pakistan Journal of Pharmaceutical Sciences. 2023;36(5):1367-1374. doi:10.36721/PJPS.2023.36.5.REG.1367-1374.1 .
Subošić, Branko, Kotur-Stevuljević, Jelena, Brborić, Jasmina, Janković, Tamara, Milenković, Marina, Ivković, Branka, Kostadinović, Jelena, Savić, Jelena, "Ex vivo and in vivo antioxidant activity of β-hydroxy-β-arylalkanoic acids" in Pakistan Journal of Pharmaceutical Sciences, 36, no. 5 (2023):1367-1374, https://doi.org/10.36721/PJPS.2023.36.5.REG.1367-1374.1 . .