Investigation and prediction of retention characteristics of selected set of central nervous system active compounds on mixed-mode diol stationary phase

Abstract

Investigation of the retention behavior of a wide range of analytes (43 nitrogen containing heterocyclic and guanidine derivatives such, as imidazoline and serotonin receptor ligands, or their related compounds) was performed on the mixed-mode stationary phase in the combined reversed-phase (RP) and hydrophilic interaction liquid chromatography (HILIC) modes. The imidazoline receptor ligands are the imidazoline or guanidine analogues with numerous therapeutic applications (such, as antihypertensives, diuretics, antiallergenics, antidiabetics, and antipsychotics), while the serotonin receptor ligands are the piperazine derivatives which exert an effect on positive and negative symptoms of schizophrenia, mania and mixed states of bipolar disorder. On the mixed-mode stationary phase, the retention behaviour of investigated compounds was described as a function of the aqueous eluent volume fractions, φ(aq), and total polarity of mobile phase (Ptot). The turning point was discussed based on different mobile phase characteristics representing the shift between the HILIC and the RP chromatographic mode. The influence of molecular properties on the main retention characteristics (turining point and the extrapolated retention parameters) is going to be discussed.