Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites
Само за регистроване кориснике
2023
Аутори
Milutinović, VioletaPetrović, Predrag
Petković, Miloš
Klaus, Anita
Ušjak, Ljuboš
Niketić, Marjan
Petrović, Silvana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the te...sted extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).
Кључне речи:
polyphenols / enzymes / Hieracium extracts / phytochemistry / terpenoidsИзвор:
Chemistry and Biodiversity, 2023, 20, 11, e202301044-Издавач:
- John Wiley and Sons Inc
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200287 (Иновациони центар Технолошко-металуршког факултета у Београду доо) (RS-MESTD-inst-2020-200287)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200116 (Универзитет у Београду, Пољопривредни факултет) (RS-MESTD-inst-2020-200116)
DOI: 10.1002/cbdv.202301044
ISSN: 1612-1872
PubMed: 37772689
Scopus: 2-s2.0-85174563128
Институција/група
PharmacyTY - JOUR AU - Milutinović, Violeta AU - Petrović, Predrag AU - Petković, Miloš AU - Klaus, Anita AU - Ušjak, Ljuboš AU - Niketić, Marjan AU - Petrović, Silvana PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5180 AB - The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM). PB - John Wiley and Sons Inc T2 - Chemistry and Biodiversity T1 - Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites VL - 20 IS - 11 SP - e202301044 DO - 10.1002/cbdv.202301044 ER -
@article{ author = "Milutinović, Violeta and Petrović, Predrag and Petković, Miloš and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana", year = "2023", abstract = "The composition and anticholinesterase activity of the dried MeOH extracts of Hieracium scheppigianum and H. naegelianum underground parts (rhizomes and roots), as well as the anticholinesterase activity of the dried, previously chemically characterised MeOH extracts of the flowering aerial parts of these two and 26 other Hieracium species in the strict sense (s. str.), were investigated. Furthermore, the anticholinesterase activity of 12 selected secondary metabolites of these extracts was evaluated. Using semi-preparative LC-MS, five caffeoylquinic acids and the sesquiterpene lactone crepiside E were isolated from H. scheppigianum underground parts extract. All these compounds were also identified in the underground parts extract of H. naegelianum. Quantitative LC-MS analysis showed that the analysed underground parts extracts were rich in both caffeoylquinic acids (139.77 and 156.62 mg/g of extract, respectively) and crepiside E (126.88 and 116.58 mg/g). In the Ellman method, the tested extracts showed an interesting anti-AChE and/or anti-BChE activity (IC50=0.56–1.58 mg/mL), which can be explained, at least partially, by the presence of some of their constituents. Among the metabolites tested, the best activity was revealed for the flavonoids apigenin, luteolin and diosmetin, and the sesquiterpene lactone 8-epiixerisamine A (IC50=68.09–299.37 μM).", publisher = "John Wiley and Sons Inc", journal = "Chemistry and Biodiversity", title = "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites", volume = "20", number = "11", pages = "e202301044", doi = "10.1002/cbdv.202301044" }
Milutinović, V., Petrović, P., Petković, M., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2023). Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity John Wiley and Sons Inc., 20(11), e202301044. https://doi.org/10.1002/cbdv.202301044
Milutinović V, Petrović P, Petković M, Klaus A, Ušjak L, Niketić M, Petrović S. Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites. in Chemistry and Biodiversity. 2023;20(11):e202301044. doi:10.1002/cbdv.202301044 .
Milutinović, Violeta, Petrović, Predrag, Petković, Miloš, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Investigation of Anticholinesterase Activity of Chemically Characterised Hieracium s. str. Methanol Extracts and Their Selected Metabolites" in Chemistry and Biodiversity, 20, no. 11 (2023):e202301044, https://doi.org/10.1002/cbdv.202301044 . .