Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC
Апстракт
The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in ...cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
Кључне речи:
Dihidroqercetin / Biochaga / antigenotoxic / DNA damage / comet assayИзвор:
10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book, 2019, 169-169Издавач:
- Turkish Society of Toxicology
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
Институција/група
PharmacyTY - CONF AU - Živković, Lada AU - Bajić, Vladan AU - Topalović, Dijana AU - Bruić, Marija AU - Spremo-Potparević, Biljana PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5187 AB - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro. PB - Turkish Society of Toxicology C3 - 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book T1 - Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC SP - 169 EP - 169 UR - https://hdl.handle.net/21.15107/rcub_farfar_5187 ER -
@conference{ author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana", year = "2019", abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements, provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding to possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in the cooperation to Dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and Dihidroqercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in cooperation. Afterwards, antigenotoxic potency of both supplements (compounds) against oxidative hydrogen peroxide (H2 O2 ) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and Dihydroquercetin displayed a strong potential to attenuate H2 O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p<0.05), whereas Biochaga at the dose of 500 μg/mL in the cooperation with Dihydroquercetin 500 μg/mL was most prominent. Biochaga in cooperation with Dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.", publisher = "Turkish Society of Toxicology", journal = "10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book", title = "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC", pages = "169-169", url = "https://hdl.handle.net/21.15107/rcub_farfar_5187" }
Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book Turkish Society of Toxicology., 169-169. https://hdl.handle.net/21.15107/rcub_farfar_5187
Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC. in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book. 2019;:169-169. https://hdl.handle.net/21.15107/rcub_farfar_5187 .
Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic effects of Biochaga and Dihydroquercetin (taxifolin) on H2O2 induced DNA damage in human WBC" in 10th International Congress of the Turkish Society of Toxicology, 16-19 October 2019 - Antalya, Turkey, Abstract book (2019):169-169, https://hdl.handle.net/21.15107/rcub_farfar_5187 .