Investigation of retention behavior of imidazoline receptor ligands on mixedmode HILIC stationary phase

Abstract

Retention mechanism in the hydrophilic interaction liquid chromatography (HILIC) mode is complex and still a subject of many controversial interpretations. In order to describe the HILIC retention mechanism, different retention models can be employed. In this study, we investigated the partition mechanism for 7 imidazoline receptor ligands on the mixed mode HILIC stationary phase. Applicability of the assumed retention model in description of the HILIC retention behavior of the compounds was successfully demonstrated.