Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay
Аутори
Bruić, MarijaGrujić-Milanović, Jelica
Živković, Lada
Topalović, Dijana
Spremo-Potparević, Biljana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Hypertension is one of the primary risk factors for heart disease and stroke, the leading causes of death worldwide. Numerous factors have been implicated in the pathophysiology of hypertension: endothelial dysfunction, arterial remodeling and vascular inflammation. Common to all these processes is increased bioavailability of reactive oxygen species in the vessels, heart, brain and kidneys. Oxidative stress and increased reactive oxygen species levels damage all macromolecules, with DNA being particularly susceptible to oxidative damage. The aim of this study was to determine whether there is a difference in the level of DNA damage between normotensive and hypertensive rats using the alkaline comet assay. Blood samples and cells suspension from liver and kidney from three male spontaneously hypertensive rats were obtained. Three normotensive male Wistar rats were used as a control. Increased level of DNA damage was detected in blood and both of the studied tissues of hypertensive rats... compared to the control, where significant difference was present in the liver and kidney cell suspensions. These results indicate that untreated hypertension in rats leads to an increased DNA damage in all of the studied samples, detected by comet assay.
Кључне речи:
DNA damage / comet assay / hypertensive rats / liver / kidneyИзвор:
Genetics & Applications, 2019, 3, 2, 89-89Издавач:
- Institute for Genetic Engineering and Biotechnology, University of Sarajevo
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
Напомена:
- 1st Congress of Geneticists in Bosnia and Herzegovina with International Participation Sarajevo, Bosnia and Herzegovina, 02nd-04th October, 2019, Book of abstracts
Институција/група
PharmacyTY - CONF AU - Bruić, Marija AU - Grujić-Milanović, Jelica AU - Živković, Lada AU - Topalović, Dijana AU - Spremo-Potparević, Biljana PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5194 AB - Hypertension is one of the primary risk factors for heart disease and stroke, the leading causes of death worldwide. Numerous factors have been implicated in the pathophysiology of hypertension: endothelial dysfunction, arterial remodeling and vascular inflammation. Common to all these processes is increased bioavailability of reactive oxygen species in the vessels, heart, brain and kidneys. Oxidative stress and increased reactive oxygen species levels damage all macromolecules, with DNA being particularly susceptible to oxidative damage. The aim of this study was to determine whether there is a difference in the level of DNA damage between normotensive and hypertensive rats using the alkaline comet assay. Blood samples and cells suspension from liver and kidney from three male spontaneously hypertensive rats were obtained. Three normotensive male Wistar rats were used as a control. Increased level of DNA damage was detected in blood and both of the studied tissues of hypertensive rats compared to the control, where significant difference was present in the liver and kidney cell suspensions. These results indicate that untreated hypertension in rats leads to an increased DNA damage in all of the studied samples, detected by comet assay. PB - Institute for Genetic Engineering and Biotechnology, University of Sarajevo C3 - Genetics & Applications T1 - Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay VL - 3 IS - 2 SP - 89 EP - 89 UR - https://hdl.handle.net/21.15107/rcub_farfar_5194 ER -
@conference{ author = "Bruić, Marija and Grujić-Milanović, Jelica and Živković, Lada and Topalović, Dijana and Spremo-Potparević, Biljana", year = "2019", abstract = "Hypertension is one of the primary risk factors for heart disease and stroke, the leading causes of death worldwide. Numerous factors have been implicated in the pathophysiology of hypertension: endothelial dysfunction, arterial remodeling and vascular inflammation. Common to all these processes is increased bioavailability of reactive oxygen species in the vessels, heart, brain and kidneys. Oxidative stress and increased reactive oxygen species levels damage all macromolecules, with DNA being particularly susceptible to oxidative damage. The aim of this study was to determine whether there is a difference in the level of DNA damage between normotensive and hypertensive rats using the alkaline comet assay. Blood samples and cells suspension from liver and kidney from three male spontaneously hypertensive rats were obtained. Three normotensive male Wistar rats were used as a control. Increased level of DNA damage was detected in blood and both of the studied tissues of hypertensive rats compared to the control, where significant difference was present in the liver and kidney cell suspensions. These results indicate that untreated hypertension in rats leads to an increased DNA damage in all of the studied samples, detected by comet assay.", publisher = "Institute for Genetic Engineering and Biotechnology, University of Sarajevo", journal = "Genetics & Applications", title = "Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay", volume = "3", number = "2", pages = "89-89", url = "https://hdl.handle.net/21.15107/rcub_farfar_5194" }
Bruić, M., Grujić-Milanović, J., Živković, L., Topalović, D.,& Spremo-Potparević, B.. (2019). Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay. in Genetics & Applications Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 3(2), 89-89. https://hdl.handle.net/21.15107/rcub_farfar_5194
Bruić M, Grujić-Milanović J, Živković L, Topalović D, Spremo-Potparević B. Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay. in Genetics & Applications. 2019;3(2):89-89. https://hdl.handle.net/21.15107/rcub_farfar_5194 .
Bruić, Marija, Grujić-Milanović, Jelica, Živković, Lada, Topalović, Dijana, Spremo-Potparević, Biljana, "Assessment of DNA damage in blood, liver and kidney cells in a hypertensive rat model using comet assay" in Genetics & Applications, 3, no. 2 (2019):89-89, https://hdl.handle.net/21.15107/rcub_farfar_5194 .