Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations
Određivanje alfa lipoinske kiseline u dijetetskim suplementima i doziranim oblicima
Аутори
Ravanić, NinaNikolić, Katarina
Popović, Gordana
Vovk, Irena
Simonovska, Breda
Filipić, Slavica
Agbaba, Danica
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Alfa lipoinska (tioktinska) kiselina se koristi u lečenju dijabetesne polineuropatije. Zbog svojih antioksidativnih svojstava, prisutna je u brojnim dijetetskim suplementima sama, kao i u kombinaciji sa aminokiselinama, L-karnitinom i drugim jedinjenjima. Dostupni podaci za kvantitativno određivanje alfa lipoinske kiseline su malobrojni. Usled toga, cilja našeg rada bio je da se razvije i validira TLC metoda za određivanje alfa lipoinske kiseline posle derivatizacije sa paladijum (II) hloridom.
Za razdvajanje alfa lipoinske kiseline i njenog redukovanog oblika korišćene su RPTLC ploče veličine 20×10 cm, uz mobilnu fazu propanol-2 : methanol : aceton : voda : sirćetna kiselina u odnosu 6:4:2:8:0,2 v/v/v/v/v. Nakon razvijanja, hromatografske ploče su potapane u rastvor paladijum (II) hlorida, a žute zone formiranog kompleksa su merene na 375 nm. Retenciona vremena alfa lipoinske kiseline i njene redukovane forme su 45 i 32 nm. Zavisnost površine signala i količine nanete supstance ispi...tana je korišćenjem linearne regresione jednačine za opseg koncentracija 1 – 3 μg i polinomalne regresione jednačine drugog stepena za koncentracioni opseg 0,5 – 5 μg. Za datu metodu dobijene vrednosti koeficijenta korelacije (r=0,999), limit kvantifikacije (0,3 μg), rikaveri (98,5 – 105,2%) i preciznost (0,9 – 2,9%) su zadovoljavajući.
Validirana hromatografska metoda je primenjena za određivanje alfa lipoinske kiseline u doziranim oblicima i dijetetskim suplementima. Nađeni sadržaj lipoinske kiseline (98,5 – 102,0%) u doziranim oblicima je u propisanim granicama, dok je u dijetetskim suplementima varirao u rasponu od 50 – 185%.
Alpha lipoic (thioctic) acid is a drug used for the treatment of diabetic polyneuropathy. Due to its antioxidant properties alpha lipoic acid nowadays widely used in dietary supplement preparation alone and in combination with amino acids, L-carnitine and other compounds. There are not so many data available on the quantitative determination of alpha lipoic acid in dietary supplements. Therefore, the aim of these investigations was to develop and validated TLC method for determination of alpha lipoic acid after derivatization by Palladium (II) chloride reagent.
The separation of alpha lipoic acid was performed on RPTLC plates (20 × 10 cm) using propanol-2 : methanol : acetone : water : acetic acid (6:4:2:8:0.2 v/v/v/v/v) as mobile phase. The plates were immersed in solutions of Palladium (II) chloride reagents and yellow spots were scanned at 375 nm. The retention times of alpha lipoic acid and its reduced form were 45 and 32 mm, respectively. Relationship of the peak areas and the a...mount of the substance applied was evaluated using the linear (1 -3 μg/spot) and second degree polynomial regression function (0.5 – 5 μg/spot). For the proposed procedure coefficient of correlation (r=0.999), limit of quantification (0.3 μg/spot), recovery (98.5 – 105.2 %) and precision (0.9 – 2.9%) were found to be satisfactory.
The developed method was applied for determination of alpha lipoic acid in drugs dosage formulations and in dietary supplement preparation. The content of lipoic acid were found to be 98.5 – 102.0% in drug dosage formulations and 50 – 185.0% in some of dietary supplement preparations.
Кључне речи:
TLC / Alpha lipoic acidИзвор:
Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti, 2009, 139-140Издавач:
- Udruženje za medicinu sporta Srbije
- Institut za bromatologiju Farmaceutskog fakulteta u Beogradu
Финансирање / пројекти:
- Синтеза, квантитативни односи између структуре/особина и активности, физичко-хемијска карактеризација и анализа фармаколошки активних супстанци (RS-MESTD-MPN2006-2010-142071)
Напомена:
- Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, 10-12. decembar 2009., Beograd, Srbija
Институција/група
PharmacyTY - CONF AU - Ravanić, Nina AU - Nikolić, Katarina AU - Popović, Gordana AU - Vovk, Irena AU - Simonovska, Breda AU - Filipić, Slavica AU - Agbaba, Danica PY - 2009 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5205 AB - Alfa lipoinska (tioktinska) kiselina se koristi u lečenju dijabetesne polineuropatije. Zbog svojih antioksidativnih svojstava, prisutna je u brojnim dijetetskim suplementima sama, kao i u kombinaciji sa aminokiselinama, L-karnitinom i drugim jedinjenjima. Dostupni podaci za kvantitativno određivanje alfa lipoinske kiseline su malobrojni. Usled toga, cilja našeg rada bio je da se razvije i validira TLC metoda za određivanje alfa lipoinske kiseline posle derivatizacije sa paladijum (II) hloridom. Za razdvajanje alfa lipoinske kiseline i njenog redukovanog oblika korišćene su RPTLC ploče veličine 20×10 cm, uz mobilnu fazu propanol-2 : methanol : aceton : voda : sirćetna kiselina u odnosu 6:4:2:8:0,2 v/v/v/v/v. Nakon razvijanja, hromatografske ploče su potapane u rastvor paladijum (II) hlorida, a žute zone formiranog kompleksa su merene na 375 nm. Retenciona vremena alfa lipoinske kiseline i njene redukovane forme su 45 i 32 nm. Zavisnost površine signala i količine nanete supstance ispitana je korišćenjem linearne regresione jednačine za opseg koncentracija 1 – 3 μg i polinomalne regresione jednačine drugog stepena za koncentracioni opseg 0,5 – 5 μg. Za datu metodu dobijene vrednosti koeficijenta korelacije (r=0,999), limit kvantifikacije (0,3 μg), rikaveri (98,5 – 105,2%) i preciznost (0,9 – 2,9%) su zadovoljavajući. Validirana hromatografska metoda je primenjena za određivanje alfa lipoinske kiseline u doziranim oblicima i dijetetskim suplementima. Nađeni sadržaj lipoinske kiseline (98,5 – 102,0%) u doziranim oblicima je u propisanim granicama, dok je u dijetetskim suplementima varirao u rasponu od 50 – 185%. AB - Alpha lipoic (thioctic) acid is a drug used for the treatment of diabetic polyneuropathy. Due to its antioxidant properties alpha lipoic acid nowadays widely used in dietary supplement preparation alone and in combination with amino acids, L-carnitine and other compounds. There are not so many data available on the quantitative determination of alpha lipoic acid in dietary supplements. Therefore, the aim of these investigations was to develop and validated TLC method for determination of alpha lipoic acid after derivatization by Palladium (II) chloride reagent. The separation of alpha lipoic acid was performed on RPTLC plates (20 × 10 cm) using propanol-2 : methanol : acetone : water : acetic acid (6:4:2:8:0.2 v/v/v/v/v) as mobile phase. The plates were immersed in solutions of Palladium (II) chloride reagents and yellow spots were scanned at 375 nm. The retention times of alpha lipoic acid and its reduced form were 45 and 32 mm, respectively. Relationship of the peak areas and the amount of the substance applied was evaluated using the linear (1 -3 μg/spot) and second degree polynomial regression function (0.5 – 5 μg/spot). For the proposed procedure coefficient of correlation (r=0.999), limit of quantification (0.3 μg/spot), recovery (98.5 – 105.2 %) and precision (0.9 – 2.9%) were found to be satisfactory. The developed method was applied for determination of alpha lipoic acid in drugs dosage formulations and in dietary supplement preparation. The content of lipoic acid were found to be 98.5 – 102.0% in drug dosage formulations and 50 – 185.0% in some of dietary supplement preparations. PB - Udruženje za medicinu sporta Srbije PB - Institut za bromatologiju Farmaceutskog fakulteta u Beogradu C3 - Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti T1 - Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations T1 - Određivanje alfa lipoinske kiseline u dijetetskim suplementima i doziranim oblicima SP - 139 EP - 140 UR - https://hdl.handle.net/21.15107/rcub_farfar_5205 ER -
@conference{ author = "Ravanić, Nina and Nikolić, Katarina and Popović, Gordana and Vovk, Irena and Simonovska, Breda and Filipić, Slavica and Agbaba, Danica", year = "2009", abstract = "Alfa lipoinska (tioktinska) kiselina se koristi u lečenju dijabetesne polineuropatije. Zbog svojih antioksidativnih svojstava, prisutna je u brojnim dijetetskim suplementima sama, kao i u kombinaciji sa aminokiselinama, L-karnitinom i drugim jedinjenjima. Dostupni podaci za kvantitativno određivanje alfa lipoinske kiseline su malobrojni. Usled toga, cilja našeg rada bio je da se razvije i validira TLC metoda za određivanje alfa lipoinske kiseline posle derivatizacije sa paladijum (II) hloridom. Za razdvajanje alfa lipoinske kiseline i njenog redukovanog oblika korišćene su RPTLC ploče veličine 20×10 cm, uz mobilnu fazu propanol-2 : methanol : aceton : voda : sirćetna kiselina u odnosu 6:4:2:8:0,2 v/v/v/v/v. Nakon razvijanja, hromatografske ploče su potapane u rastvor paladijum (II) hlorida, a žute zone formiranog kompleksa su merene na 375 nm. Retenciona vremena alfa lipoinske kiseline i njene redukovane forme su 45 i 32 nm. Zavisnost površine signala i količine nanete supstance ispitana je korišćenjem linearne regresione jednačine za opseg koncentracija 1 – 3 μg i polinomalne regresione jednačine drugog stepena za koncentracioni opseg 0,5 – 5 μg. Za datu metodu dobijene vrednosti koeficijenta korelacije (r=0,999), limit kvantifikacije (0,3 μg), rikaveri (98,5 – 105,2%) i preciznost (0,9 – 2,9%) su zadovoljavajući. Validirana hromatografska metoda je primenjena za određivanje alfa lipoinske kiseline u doziranim oblicima i dijetetskim suplementima. Nađeni sadržaj lipoinske kiseline (98,5 – 102,0%) u doziranim oblicima je u propisanim granicama, dok je u dijetetskim suplementima varirao u rasponu od 50 – 185%., Alpha lipoic (thioctic) acid is a drug used for the treatment of diabetic polyneuropathy. Due to its antioxidant properties alpha lipoic acid nowadays widely used in dietary supplement preparation alone and in combination with amino acids, L-carnitine and other compounds. There are not so many data available on the quantitative determination of alpha lipoic acid in dietary supplements. Therefore, the aim of these investigations was to develop and validated TLC method for determination of alpha lipoic acid after derivatization by Palladium (II) chloride reagent. The separation of alpha lipoic acid was performed on RPTLC plates (20 × 10 cm) using propanol-2 : methanol : acetone : water : acetic acid (6:4:2:8:0.2 v/v/v/v/v) as mobile phase. The plates were immersed in solutions of Palladium (II) chloride reagents and yellow spots were scanned at 375 nm. The retention times of alpha lipoic acid and its reduced form were 45 and 32 mm, respectively. Relationship of the peak areas and the amount of the substance applied was evaluated using the linear (1 -3 μg/spot) and second degree polynomial regression function (0.5 – 5 μg/spot). For the proposed procedure coefficient of correlation (r=0.999), limit of quantification (0.3 μg/spot), recovery (98.5 – 105.2 %) and precision (0.9 – 2.9%) were found to be satisfactory. The developed method was applied for determination of alpha lipoic acid in drugs dosage formulations and in dietary supplement preparation. The content of lipoic acid were found to be 98.5 – 102.0% in drug dosage formulations and 50 – 185.0% in some of dietary supplement preparations.", publisher = "Udruženje za medicinu sporta Srbije, Institut za bromatologiju Farmaceutskog fakulteta u Beogradu", journal = "Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti", title = "Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations, Određivanje alfa lipoinske kiseline u dijetetskim suplementima i doziranim oblicima", pages = "139-140", url = "https://hdl.handle.net/21.15107/rcub_farfar_5205" }
Ravanić, N., Nikolić, K., Popović, G., Vovk, I., Simonovska, B., Filipić, S.,& Agbaba, D.. (2009). Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations. in Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti Udruženje za medicinu sporta Srbije., 139-140. https://hdl.handle.net/21.15107/rcub_farfar_5205
Ravanić N, Nikolić K, Popović G, Vovk I, Simonovska B, Filipić S, Agbaba D. Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations. in Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti. 2009;:139-140. https://hdl.handle.net/21.15107/rcub_farfar_5205 .
Ravanić, Nina, Nikolić, Katarina, Popović, Gordana, Vovk, Irena, Simonovska, Breda, Filipić, Slavica, Agbaba, Danica, "Determination of alpha lipoic acid in dietary supplement preparations and in drug formulations" in Drugi kongres o dijetetskim suplementima sa međunarodnim učešćem, Apstrakti (2009):139-140, https://hdl.handle.net/21.15107/rcub_farfar_5205 .