Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers
Аутори
Čabarkapa, AndreaŽivković, Lada
Dekanski, Dragana
Topalović, Dijana
Bajić, Vladan
Spremo-Potparević, Biljana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers... to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
Кључне речи:
DNA damage / comet assay / DOLE / CaNa2EDTA / Dry olive leaf extractИзвор:
International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book, 2016, 135-135Издавач:
- International Congress on Occupational & Environmental Toxicology - ICOETox 2016
Финансирање / пројекти:
- Аберације ћелијског циклуса и утицај оксидативног стреса на неуродегенеративне процесе и малигну трансформацију ћелије (RS-MESTD-Basic Research (BR or ON)-173034)
Напомена:
- 3rd International Congress on Occupational & Environmental Toxicology - ICOETox 2016 and 3rd Ibero-American Meeting on Toxicology and Environmental Health International (IBAMTOX 2016), 21-23 June 2016 | Porto
Институција/група
PharmacyTY - CONF AU - Čabarkapa, Andrea AU - Živković, Lada AU - Dekanski, Dragana AU - Topalović, Dijana AU - Bajić, Vladan AU - Spremo-Potparević, Biljana PY - 2016 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5231 AB - The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy. PB - International Congress on Occupational & Environmental Toxicology - ICOETox 2016 C3 - International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book T1 - Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers SP - 135 EP - 135 UR - https://hdl.handle.net/21.15107/rcub_farfar_5231 ER -
@conference{ author = "Čabarkapa, Andrea and Živković, Lada and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Spremo-Potparević, Biljana", year = "2016", abstract = "The CaNa2EDTA chelation is a standard therapy for lead (Pb) intoxication in occupationally exposed workers. Application of antioxidant nutrients through exogenous supplementation is often practiced with the chelation therapy, although their synergistic effect in reduction of Pb-induced oxidative damage has not been investigated conclusively. Dry olive Leaf extract (DOLE) is polyphenol rich natural antioxidant. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 male workers occupationally exposed to Pb, before and after application of five day CaNa2EDTA chelation therapy. Comet assay was used to assess levels of DNA damage. While the level of DNA damage in PBLs of workers before chelation were moderately increased (24.21±14.26) compared to controls (6.0±3.37), the incubation of the same samples with 1mg/mL DOLE for 30 min at 370C lead to a severe increase in DNA damage levels (64.03±20.96). After the exposure of workers to a five day CaNa2EDTA chelation regimen, the experiment was repeated. Following chelation, the level of DNA damage in PBLs of workers was decreased (8.26±4.62) significantly compared to the baseline value and was then similar to the control level. When the PBLs after chelation were treated with 1mg/mL DOLE for 30 min, high level of damage was obtained (41.82±23.17). The antigenotoxic effects of five day CaNa2EDTA chelation were demonstrated in PBLs of Pb exposed workers. On the contrary, the applications of DOLE lead to an increase of oxidative DNA damage after 30 min incubation, exhibiting prooxidant rather than antioxidant effect. After CaNa2EDTA treatment, the acute prooxidant effects of DOLE remained following the incubation, but, the oxidative DNA damage was less severe compared to the same experiment with DOLE before the chelation, probably as a result of partial removal of Pb from cells by chelation therapy. Prooxidant nature of DOLE could be a result of Pb-mediated hydroxyl radical formation, where heavy metals serve as catalysts for the reactions which oxidize DOLE and reduce oxygen. Removal of Pb by complexation with CaNa2EDTA seems to significantly depress these oxidative events. However, this mechanism remains to be explored on molecular level. It could be concluded that the DOLE exhibits prooxidant effects in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.", publisher = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016", journal = "International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book", title = "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers", pages = "135-135", url = "https://hdl.handle.net/21.15107/rcub_farfar_5231" }
Čabarkapa, A., Živković, L., Dekanski, D., Topalović, D., Bajić, V.,& Spremo-Potparević, B.. (2016). Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book International Congress on Occupational & Environmental Toxicology - ICOETox 2016., 135-135. https://hdl.handle.net/21.15107/rcub_farfar_5231
Čabarkapa A, Živković L, Dekanski D, Topalović D, Bajić V, Spremo-Potparević B. Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers. in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book. 2016;:135-135. https://hdl.handle.net/21.15107/rcub_farfar_5231 .
Čabarkapa, Andrea, Živković, Lada, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Spremo-Potparević, Biljana, "Unexpected effect of dry olive leaf extract (DOLE) before and after CaNa2EDTA chelation therapy in comet assay in lead intoxicated workers" in International Congress on Occupational & Environmental Toxicology - ICOETox 2016, 21-23 June | Porto, Abstract book (2016):135-135, https://hdl.handle.net/21.15107/rcub_farfar_5231 .