Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition
Апстракт
Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting
dry grasslands, limestone rocky areas and screes
of wide sense Mediterranean area, east European lowlands
and Caucasus. This study explores the anatomical structure,
content and composition of the essential oil of the vegetative
organs and fruit of this species. The plant material was collected
on two localities: the village Izvor (Bosilegrad, Serbia)
and Mt. Galičica (FYR Macedonia). The anatomical studies
were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope.
The essential oils obtained by hydrodistillation from roots
(collected during flowering and fruiting period), aerial parts
with inflorescences, and fruits, were analysed by GC-FID and
GC-MS. The anatomical analysis revealed a secondary structure
of root and primary structure of stem with closed collateral
vascular bundles. Leaves are isobilateral, amphistomatic;
petiole is with arched v...ascular bundles and the fruit (mericarp)
is semi-circular and lightly ribbed in cross section.
Non-glandular unicellular and bicellular cuneate trichomes
are sparsely distributed on the stem, leaves and petiole, but
very dense on the fruit. Secretory channels are present in all
organs: in the parenchyma of the root and stem cortex, stem
pith, in the phloem of root and vascular bundles of stem and
petiole, by the leaf vascular bundles and in the fruit pericarp.
Essential oil yields from different parts of P. tragium ranged
from 0.2-1.1% (v/w). The main compounds in the oils from
roots (in both stages, from both localities), as well as from
aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes
(trinorsesquiterpenes): pregeijerene (29.0-56.2%)
and gejerene (14.1-22.9%), whereas those from aerial parts
and fruits from village Izvor are β-bisabolene (19.1-57.2%)
and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate
(17.4-22.2%). Trinorsesquiterpenes, which were also
found to be characteristic constituents in some other Pimpinella
species oils, are the most dominant constituents in all
investigated oils, except in the oil of fruit from village Izvor.
In addition, phenylpropanoids of pseudoisoeugenol type are
present in all oils confi rming them as chemical markers of
Pimpinella species analysed so far.
Кључне речи:
Pimpinella tragium / anatomy / secretory canals / essential oil / C-12 norsesquiterpenes / phenylpropanoidsИзвор:
Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 2018, 42, 1 supplement, 130-130Издавач:
- Institute of Botany and Botanical Garden Jevremovac, Belgrade
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
Напомена:
- 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia
Институција/група
PharmacyTY - CONF AU - Slavkovska, Violeta AU - Zlatković, Bojan AU - Kovačević, Nada AU - Drobac, Milica PY - 2018 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5253 AB - Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting dry grasslands, limestone rocky areas and screes of wide sense Mediterranean area, east European lowlands and Caucasus. This study explores the anatomical structure, content and composition of the essential oil of the vegetative organs and fruit of this species. The plant material was collected on two localities: the village Izvor (Bosilegrad, Serbia) and Mt. Galičica (FYR Macedonia). The anatomical studies were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope. The essential oils obtained by hydrodistillation from roots (collected during flowering and fruiting period), aerial parts with inflorescences, and fruits, were analysed by GC-FID and GC-MS. The anatomical analysis revealed a secondary structure of root and primary structure of stem with closed collateral vascular bundles. Leaves are isobilateral, amphistomatic; petiole is with arched vascular bundles and the fruit (mericarp) is semi-circular and lightly ribbed in cross section. Non-glandular unicellular and bicellular cuneate trichomes are sparsely distributed on the stem, leaves and petiole, but very dense on the fruit. Secretory channels are present in all organs: in the parenchyma of the root and stem cortex, stem pith, in the phloem of root and vascular bundles of stem and petiole, by the leaf vascular bundles and in the fruit pericarp. Essential oil yields from different parts of P. tragium ranged from 0.2-1.1% (v/w). The main compounds in the oils from roots (in both stages, from both localities), as well as from aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes (trinorsesquiterpenes): pregeijerene (29.0-56.2%) and gejerene (14.1-22.9%), whereas those from aerial parts and fruits from village Izvor are β-bisabolene (19.1-57.2%) and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate (17.4-22.2%). Trinorsesquiterpenes, which were also found to be characteristic constituents in some other Pimpinella species oils, are the most dominant constituents in all investigated oils, except in the oil of fruit from village Izvor. In addition, phenylpropanoids of pseudoisoeugenol type are present in all oils confi rming them as chemical markers of Pimpinella species analysed so far. PB - Institute of Botany and Botanical Garden Jevremovac, Belgrade C3 - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts T1 - Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition VL - 42 IS - 1 supplement SP - 130 EP - 130 UR - https://hdl.handle.net/21.15107/rcub_farfar_5253 ER -
@conference{ author = "Slavkovska, Violeta and Zlatković, Bojan and Kovačević, Nada and Drobac, Milica", year = "2018", abstract = "Pimpinella tragium Vill. is a perennial, herbaceous plant inhabiting dry grasslands, limestone rocky areas and screes of wide sense Mediterranean area, east European lowlands and Caucasus. This study explores the anatomical structure, content and composition of the essential oil of the vegetative organs and fruit of this species. The plant material was collected on two localities: the village Izvor (Bosilegrad, Serbia) and Mt. Galičica (FYR Macedonia). The anatomical studies were conducted on permanent slides obtained by standard method of preparation for viewing under a light microscope. The essential oils obtained by hydrodistillation from roots (collected during flowering and fruiting period), aerial parts with inflorescences, and fruits, were analysed by GC-FID and GC-MS. The anatomical analysis revealed a secondary structure of root and primary structure of stem with closed collateral vascular bundles. Leaves are isobilateral, amphistomatic; petiole is with arched vascular bundles and the fruit (mericarp) is semi-circular and lightly ribbed in cross section. Non-glandular unicellular and bicellular cuneate trichomes are sparsely distributed on the stem, leaves and petiole, but very dense on the fruit. Secretory channels are present in all organs: in the parenchyma of the root and stem cortex, stem pith, in the phloem of root and vascular bundles of stem and petiole, by the leaf vascular bundles and in the fruit pericarp. Essential oil yields from different parts of P. tragium ranged from 0.2-1.1% (v/w). The main compounds in the oils from roots (in both stages, from both localities), as well as from aerial parts and fruits from Mt. Galičica are C-12 norsesquiterpenes (trinorsesquiterpenes): pregeijerene (29.0-56.2%) and gejerene (14.1-22.9%), whereas those from aerial parts and fruits from village Izvor are β-bisabolene (19.1-57.2%) and a phenylpropanoid epoxy-pseudoisoeugenyl-2-metohybutyrate (17.4-22.2%). Trinorsesquiterpenes, which were also found to be characteristic constituents in some other Pimpinella species oils, are the most dominant constituents in all investigated oils, except in the oil of fruit from village Izvor. In addition, phenylpropanoids of pseudoisoeugenol type are present in all oils confi rming them as chemical markers of Pimpinella species analysed so far.", publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade", journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts", title = "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition", volume = "42", number = "1 supplement", pages = "130-130", url = "https://hdl.handle.net/21.15107/rcub_farfar_5253" }
Slavkovska, V., Zlatković, B., Kovačević, N.,& Drobac, M.. (2018). Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 130-130. https://hdl.handle.net/21.15107/rcub_farfar_5253
Slavkovska V, Zlatković B, Kovačević N, Drobac M. Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):130-130. https://hdl.handle.net/21.15107/rcub_farfar_5253 .
Slavkovska, Violeta, Zlatković, Bojan, Kovačević, Nada, Drobac, Milica, "Pimpinella tragium Vill. (Apiaceae) – anatomy and essential oil composition" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):130-130, https://hdl.handle.net/21.15107/rcub_farfar_5253 .