Maceration and heat-assisted extraction of polyphenols from Aloe vera
Аутори
Čutović, NatalijaJovanović, Aleksandra
Rajab Elferjane, Rajab Elferjane
Milutinović, Violeta
Petrović, Predrag
Marinković, Aleksandar
Bugarski, Branko
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids,
tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes,
vitamins, and minerals. The plant is used in traditional medicine due to its antitumor,
antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal
protection properties. Maceration and heat-assisted extraction (HAE) are frequently used
extraction procedures because of their simple operation and low costs. In the present study, A.
vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The
influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and
various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content
(TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the
most prominent extracts (with the highest TPC) zeta pot...ential and conductivity were additionally
analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to
45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and
after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant
differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32,
9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min
showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the
increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg
GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different
between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol
Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different
extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract
obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration
when also reached the steady state (~40.4 mg/mL), but there were no significant differences
between the extracts prepared at different extraction times in HAE, and IC50 was lower for the
extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained
after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and
conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and
cosmetics.
Кључне речи:
Aloe vera / antiradical activity / conductivity / polyphenols / zeta potentialИзвор:
VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings, 2023, 136-143Издавач:
- University of East Sarajevo, Faculty of technology
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200135 (Универзитет у Београду, Технолошко-металуршки факултет) (RS-MESTD-inst-2020-200135)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200003 (Институт за проучавање лековитог биља 'Др Јосиф Панчић', Београд) (RS-MESTD-inst-2020-200003)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
Напомена:
- VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina. Saopštenje sa međunarodnog skupa štampano u celini.
Институција/група
PharmacyTY - CONF AU - Čutović, Natalija AU - Jovanović, Aleksandra AU - Rajab Elferjane, Rajab Elferjane AU - Milutinović, Violeta AU - Petrović, Predrag AU - Marinković, Aleksandar AU - Bugarski, Branko PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5261 AB - Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal protection properties. Maceration and heat-assisted extraction (HAE) are frequently used extraction procedures because of their simple operation and low costs. In the present study, A. vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content (TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration when also reached the steady state (~40.4 mg/mL), but there were no significant differences between the extracts prepared at different extraction times in HAE, and IC50 was lower for the extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and cosmetics. PB - University of East Sarajevo, Faculty of technology C3 - VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings T1 - Maceration and heat-assisted extraction of polyphenols from Aloe vera SP - 136 EP - 143 UR - https://hdl.handle.net/21.15107/rcub_farfar_5261 ER -
@conference{ author = "Čutović, Natalija and Jovanović, Aleksandra and Rajab Elferjane, Rajab Elferjane and Milutinović, Violeta and Petrović, Predrag and Marinković, Aleksandar and Bugarski, Branko", year = "2023", abstract = "Aloe vera (L.) Burm. f. (Asphodelaceae) contains anthraquinones, their glycosides, flavonoids, tannins, terpenoids, saponins, resins, mono- and polysaccharides, polypeptides, lectins, enzymes, vitamins, and minerals. The plant is used in traditional medicine due to its antitumor, antioxidant, anti-inflammatory, antiulcerogenic, immunomodulatory, analgesic, and dermal protection properties. Maceration and heat-assisted extraction (HAE) are frequently used extraction procedures because of their simple operation and low costs. In the present study, A. vera dried leaves were extracted using ethanol (50%, V/V; drug solvent ratio 1:30, w/V). The influence of different extraction times (30-120 min for maceration and 15-60 min for HAE), and various extraction temperatures (40, 60, and 80ºC) in HAE on the total polyphenol content (TPC) and radical scavenging potential (ABTS and DPPH methods) was evaluated, while for the most prominent extracts (with the highest TPC) zeta potential and conductivity were additionally analyzed. Regarding maceration, the TPC was correlated with the rise in extraction time up to 45 min (from 7.8±0.72 to 9.66±0.51 mg gallic acid equivalents (GAE)/g of plant material) and after that reached the steady state (~9.1 mg GAE/g). In HAE, no statistically significant differences between the TPC of the extracts obtained after 15, 30, and 45 min (9.77±0.32, 9.76±0.18, and 9.95±0.50 mg GAE/g) were observed, while the extract prepared after 60 min showed significantly lower TPC (8.14±0.08 mg GAE/g). The TPC was in correlation with the increase of extraction temperatures (9.08±0.21 at 40ºC, 9.38±0.10 at 60ºC, and 9.86±0.24 mg GAE/g at 80ºC). As in the case of TPC results, anti-ABTS activity was significantly different between 30 and 45 min of maceration and reached the steady state after 45 min (~2.01 mmol Trolox/g of plant material). In HAE, the anti-ABTS potential of the extracts obtained at different extraction times was comparable (1.44-1.64 mmol Trolox/g), whereas the activity of the extract obtained at 40ºC was lower. DPPH radical scavenging activity rose after 45 min of maceration when also reached the steady state (~40.4 mg/mL), but there were no significant differences between the extracts prepared at different extraction times in HAE, and IC50 was lower for the extract obtained at 80ºC. Hence, the macerate prepared after 45 min and HAE extract obtained after 15 min at 80ºC exhibited very low zeta potential (0.14±0.06 and 0.50±0.01 mV), and conductivity (1.05±0.07 and 0.98±0.01 mS/cm). This study was an initial step in the production of A. vera polyphenol extracts aimed to be used for the formulation of foodstuffs, medicines, and cosmetics.", publisher = "University of East Sarajevo, Faculty of technology", journal = "VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings", title = "Maceration and heat-assisted extraction of polyphenols from Aloe vera", pages = "136-143", url = "https://hdl.handle.net/21.15107/rcub_farfar_5261" }
Čutović, N., Jovanović, A., Rajab Elferjane, R. E., Milutinović, V., Petrović, P., Marinković, A.,& Bugarski, B.. (2023). Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings University of East Sarajevo, Faculty of technology., 136-143. https://hdl.handle.net/21.15107/rcub_farfar_5261
Čutović N, Jovanović A, Rajab Elferjane RE, Milutinović V, Petrović P, Marinković A, Bugarski B. Maceration and heat-assisted extraction of polyphenols from Aloe vera. in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings. 2023;:136-143. https://hdl.handle.net/21.15107/rcub_farfar_5261 .
Čutović, Natalija, Jovanović, Aleksandra, Rajab Elferjane, Rajab Elferjane, Milutinović, Violeta, Petrović, Predrag, Marinković, Aleksandar, Bugarski, Branko, "Maceration and heat-assisted extraction of polyphenols from Aloe vera" in VIII International Congress “Engineering, Environment and Materials in Process Industry-EEM2023", Jahorina, March 20-23, 2023, Republic of Srpska, Bosnia and Herzegovina, Proceedings (2023):136-143, https://hdl.handle.net/21.15107/rcub_farfar_5261 .