Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)
Апстракт
Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits
central parts of Serbia and Kosovo. The genus Rumex is characterizied by the
accumulation of anthraquinones, naphthalene-1,8-diols, flavonoids and stilbenoids.
Aerial parts of R. balcanicus, collected on Vlasina Lake (Serbia) in 2014, were used
to determine the chemical composition and cytotoxic activity. Cyclohexane,
dichloromethane, methanol and aqueous extracts were prepared and tested using
MTT-test, against human epithelial cervical cancer cells (HeLa), human colon
carcinoma cell line (LS174), human non-small cell lung cancer cells (A549) and
healthy MRC-5 human embryonic lung fibroblast cell line. Methanol and aqueous
extracts were analyzed using LC-MS. In tested extracts, caffeic acid derivates and
flavonol derivatives were identified. The main components in both extracts were rutin,
miquelianin, quercitrin and quercetin 3-O-acetyl-rhamnoside. Caffeic acid and its
glycosides were also present... in analyzed extracts. Aqueous extracts had no
cytotoxicity against tested cell lines, while cyclohexane, dichlormethane and
methanol extracts were active against all tested cell lines with IC50 ranged from
116.48±3.26 μg/mL to 178.21±1.30 μg/mL. Due to the presence of flavonoids and
exhibited cytotoxic effect, R. balcanicus herba could be interesting as a plant material
for further pharmacological analysis and isolation of the anti-tumor compounds.
Кључне речи:
Rumex balcanicus / cytotoxity / caffeic acid derivatives / flavonolsИзвор:
13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts, 2019, 158-158Издавач:
- Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
- Institute for Nature Conservation of Serbia
Финансирање / пројекти:
- Испитивање лековитог потенцијала биљака: морфолошка, хемијска и фармаколошка карактеризација (RS-MESTD-Basic Research (BR or ON)-173021)
- Биљни и синтетички биоактивни производи новије генерације (RS-MESTD-Technological Development (TD or TR)-34012)
Напомена:
- 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Stara planina Mt., 20th to 23th June 2019
Институција/група
PharmacyTY - CONF AU - Arsenijević, Jelena AU - Stanojković, Tatjana AU - Ranđelović, Vladimir AU - Kundaković-Vasović, Tatjana PY - 2019 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5294 AB - Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. The genus Rumex is characterizied by the accumulation of anthraquinones, naphthalene-1,8-diols, flavonoids and stilbenoids. Aerial parts of R. balcanicus, collected on Vlasina Lake (Serbia) in 2014, were used to determine the chemical composition and cytotoxic activity. Cyclohexane, dichloromethane, methanol and aqueous extracts were prepared and tested using MTT-test, against human epithelial cervical cancer cells (HeLa), human colon carcinoma cell line (LS174), human non-small cell lung cancer cells (A549) and healthy MRC-5 human embryonic lung fibroblast cell line. Methanol and aqueous extracts were analyzed using LC-MS. In tested extracts, caffeic acid derivates and flavonol derivatives were identified. The main components in both extracts were rutin, miquelianin, quercitrin and quercetin 3-O-acetyl-rhamnoside. Caffeic acid and its glycosides were also present in analyzed extracts. Aqueous extracts had no cytotoxicity against tested cell lines, while cyclohexane, dichlormethane and methanol extracts were active against all tested cell lines with IC50 ranged from 116.48±3.26 μg/mL to 178.21±1.30 μg/mL. Due to the presence of flavonoids and exhibited cytotoxic effect, R. balcanicus herba could be interesting as a plant material for further pharmacological analysis and isolation of the anti-tumor compounds. PB - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš PB - Institute for Nature Conservation of Serbia C3 - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts T1 - Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae) SP - 158 EP - 158 UR - https://hdl.handle.net/21.15107/rcub_farfar_5294 ER -
@conference{ author = "Arsenijević, Jelena and Stanojković, Tatjana and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana", year = "2019", abstract = "Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. The genus Rumex is characterizied by the accumulation of anthraquinones, naphthalene-1,8-diols, flavonoids and stilbenoids. Aerial parts of R. balcanicus, collected on Vlasina Lake (Serbia) in 2014, were used to determine the chemical composition and cytotoxic activity. Cyclohexane, dichloromethane, methanol and aqueous extracts were prepared and tested using MTT-test, against human epithelial cervical cancer cells (HeLa), human colon carcinoma cell line (LS174), human non-small cell lung cancer cells (A549) and healthy MRC-5 human embryonic lung fibroblast cell line. Methanol and aqueous extracts were analyzed using LC-MS. In tested extracts, caffeic acid derivates and flavonol derivatives were identified. The main components in both extracts were rutin, miquelianin, quercitrin and quercetin 3-O-acetyl-rhamnoside. Caffeic acid and its glycosides were also present in analyzed extracts. Aqueous extracts had no cytotoxicity against tested cell lines, while cyclohexane, dichlormethane and methanol extracts were active against all tested cell lines with IC50 ranged from 116.48±3.26 μg/mL to 178.21±1.30 μg/mL. Due to the presence of flavonoids and exhibited cytotoxic effect, R. balcanicus herba could be interesting as a plant material for further pharmacological analysis and isolation of the anti-tumor compounds.", publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia", journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts", title = "Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)", pages = "158-158", url = "https://hdl.handle.net/21.15107/rcub_farfar_5294" }
Arsenijević, J., Stanojković, T., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 158-158. https://hdl.handle.net/21.15107/rcub_farfar_5294
Arsenijević J, Stanojković T, Ranđelović V, Kundaković-Vasović T. Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:158-158. https://hdl.handle.net/21.15107/rcub_farfar_5294 .
Arsenijević, Jelena, Stanojković, Tatjana, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):158-158, https://hdl.handle.net/21.15107/rcub_farfar_5294 .