Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis
Само за регистроване кориснике
2023
Аутори
Belić, MilicaSopić, Miron
Roksandić-Milenković, Marina
Ćeriman, Vesna
Guzonjić, Azra
Vukašinović, Aleksandra
Ostanek, Barbara
Dimić, Nemanja
Jovanović, Dragana
Kotur-Stevuljević, Jelena
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039...). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.
Кључне речи:
lung cancer / oxidative stress / peripheral blood telomere lengthИзвор:
Folia biologica, 2023, 69, 2, 59-68Издавач:
- Charles University, Czech Republic
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
- CEEPUS project Novel Diagnostic and Therapeutic Approaches to Complex Genetic Disorders CIII-SI-0611-08-1819
DOI: 10.14712/fb2023069020059
ISSN: 0015-5500
PubMed: 38063002
Scopus: 2-s2.0-85179641874
Институција/група
PharmacyTY - JOUR AU - Belić, Milica AU - Sopić, Miron AU - Roksandić-Milenković, Marina AU - Ćeriman, Vesna AU - Guzonjić, Azra AU - Vukašinović, Aleksandra AU - Ostanek, Barbara AU - Dimić, Nemanja AU - Jovanović, Dragana AU - Kotur-Stevuljević, Jelena PY - 2023 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5387 AB - Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression. PB - Charles University, Czech Republic T2 - Folia biologica T1 - Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis VL - 69 IS - 2 SP - 59 EP - 68 DO - 10.14712/fb2023069020059 ER -
@article{ author = "Belić, Milica and Sopić, Miron and Roksandić-Milenković, Marina and Ćeriman, Vesna and Guzonjić, Azra and Vukašinović, Aleksandra and Ostanek, Barbara and Dimić, Nemanja and Jovanović, Dragana and Kotur-Stevuljević, Jelena", year = "2023", abstract = "Lung cancer (LC) is the second most common malignancy and leading cause of cancer death. The potential "culprit" for local and systemic telomere shortening in LC patients is oxidative stress. We investigated the correlation between the peripheral blood leukocyte (PBL) telomere length (TL) and the presence/severity of LC and oxidative stress, and its usefulness as LC diagnostic marker. PBL TL was measured in 89 LC patients and 83 healthy subjects using the modified Cawthon RTq-PCR method. The relative PBL TL, found to be a potential diagnostic marker for LC with very good accuracy (P < 0.001), was significantly shorter in patients compared to the control group (CG) (P < 0.001). Significantly shorter telomeres were found in patients with LC TNM stage IV than in patients with stages I-III (P = 0.014), in patients without therapy compared to those on therapy (P = 0.008), and in patients with partial response and stable/progressive disease compared to those with complete response (P = 0.039). The total oxidant status (TOS), advanced oxidation protein products (AOPP), prooxidant-antioxidant balance (PAB) and C-reactive protein (CRP) were significantly higher in patients compared to CG (P < 0.001) and correlated negatively with TL in both patients and CG (P < 0.001). PCA showed a relation between PAB and TL, and between the EGFR status and TL. Oxidative stress and PBL telomere shortening are probably associated with LC development and progression.", publisher = "Charles University, Czech Republic", journal = "Folia biologica", title = "Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis", volume = "69", number = "2", pages = "59-68", doi = "10.14712/fb2023069020059" }
Belić, M., Sopić, M., Roksandić-Milenković, M., Ćeriman, V., Guzonjić, A., Vukašinović, A., Ostanek, B., Dimić, N., Jovanović, D.,& Kotur-Stevuljević, J.. (2023). Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis. in Folia biologica Charles University, Czech Republic., 69(2), 59-68. https://doi.org/10.14712/fb2023069020059
Belić M, Sopić M, Roksandić-Milenković M, Ćeriman V, Guzonjić A, Vukašinović A, Ostanek B, Dimić N, Jovanović D, Kotur-Stevuljević J. Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis. in Folia biologica. 2023;69(2):59-68. doi:10.14712/fb2023069020059 .
Belić, Milica, Sopić, Miron, Roksandić-Milenković, Marina, Ćeriman, Vesna, Guzonjić, Azra, Vukašinović, Aleksandra, Ostanek, Barbara, Dimić, Nemanja, Jovanović, Dragana, Kotur-Stevuljević, Jelena, "Correlation of Short Leukocyte Telomeres and Oxidative Stress with the Presence and Severity of Lung Cancer Explored by Principal Component Analysis" in Folia biologica, 69, no. 2 (2023):59-68, https://doi.org/10.14712/fb2023069020059 . .