Impact of defatting process on antioxidant potential of grape seeds
Апстракт
Grape seeds contain a wide range of phenolic compounds responsible for its health promoting
effects. Interest for grape seed utilization has increased significantly along with a growing
recognition that present biologically active compounds were not completely isolated during the
extraction process. Numerous studies have been conducted in order to achieve the best
extraction recovery. Nevertheless, there is still a question concerning the pretreatment of grape
seeds. The aim of the present study was to investigate the impact of defatting process on total
polyphenol content and antioxidant activity of grape seed extracts. For that purpose seeds were
obtained from eight different grape varieties. Extraction with 70 % ethanol was performed on
both, crude and defatted seeds (lipid fraction was removed by Soxhlet method of extraction).
Determination of total phenolic content (TPC) was done using Folin-Ciocalteu assay.
Antioxidant activity was investigated through four different te...sts (FRAP, CUPRAC, DPPH and
ABTS) and obtained results were combined in unique Antioxidant Composite Index (ACI). TPC
values ranged from 50.48 to 121.06 and from 38.04 to 108.17 mg GAE/g dry weight for crude
and defatted grape seed extracts, respectively. Higher phenolic content determined in extracts
obtained from crude seeds could be explained by the presence of lipophilic biologically active
compounds in non-removed lipid fraction. Discrepancies between varieties are presumably
present due to different morphological properties of seeds, environmental and agricultural
conditions. Concerning antioxidant activity, all tests were significantly correlated with TPC,
suggesting the strong contribution of phenolic compounds to this biological activity.
Кључне речи:
grape seeds / polyphenols / antioxidants / extraction / defatting processИзвор:
Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade, 2020, 34-34Издавач:
- University of Belgrade, Belgrade, Serbia
Финансирање / пројекти:
- Развој и примена нових и традиционалних технологија у производњи конкурентних прехрамбених производа са додатом вредношћу за европско и светско тржиште - Створимо богатство из богатства Србије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46001)
Напомена:
- Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade, March 2-3, 2020
Институција/група
PharmacyTY - CONF AU - Dabetić, Nevena AU - Todorović, Vanja AU - Vidović, Bojana AU - Šobajić, Slađana PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5397 AB - Grape seeds contain a wide range of phenolic compounds responsible for its health promoting effects. Interest for grape seed utilization has increased significantly along with a growing recognition that present biologically active compounds were not completely isolated during the extraction process. Numerous studies have been conducted in order to achieve the best extraction recovery. Nevertheless, there is still a question concerning the pretreatment of grape seeds. The aim of the present study was to investigate the impact of defatting process on total polyphenol content and antioxidant activity of grape seed extracts. For that purpose seeds were obtained from eight different grape varieties. Extraction with 70 % ethanol was performed on both, crude and defatted seeds (lipid fraction was removed by Soxhlet method of extraction). Determination of total phenolic content (TPC) was done using Folin-Ciocalteu assay. Antioxidant activity was investigated through four different tests (FRAP, CUPRAC, DPPH and ABTS) and obtained results were combined in unique Antioxidant Composite Index (ACI). TPC values ranged from 50.48 to 121.06 and from 38.04 to 108.17 mg GAE/g dry weight for crude and defatted grape seed extracts, respectively. Higher phenolic content determined in extracts obtained from crude seeds could be explained by the presence of lipophilic biologically active compounds in non-removed lipid fraction. Discrepancies between varieties are presumably present due to different morphological properties of seeds, environmental and agricultural conditions. Concerning antioxidant activity, all tests were significantly correlated with TPC, suggesting the strong contribution of phenolic compounds to this biological activity. PB - University of Belgrade, Belgrade, Serbia C3 - Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade T1 - Impact of defatting process on antioxidant potential of grape seeds SP - 34 EP - 34 UR - https://hdl.handle.net/21.15107/rcub_farfar_5397 ER -
@conference{ author = "Dabetić, Nevena and Todorović, Vanja and Vidović, Bojana and Šobajić, Slađana", year = "2020", abstract = "Grape seeds contain a wide range of phenolic compounds responsible for its health promoting effects. Interest for grape seed utilization has increased significantly along with a growing recognition that present biologically active compounds were not completely isolated during the extraction process. Numerous studies have been conducted in order to achieve the best extraction recovery. Nevertheless, there is still a question concerning the pretreatment of grape seeds. The aim of the present study was to investigate the impact of defatting process on total polyphenol content and antioxidant activity of grape seed extracts. For that purpose seeds were obtained from eight different grape varieties. Extraction with 70 % ethanol was performed on both, crude and defatted seeds (lipid fraction was removed by Soxhlet method of extraction). Determination of total phenolic content (TPC) was done using Folin-Ciocalteu assay. Antioxidant activity was investigated through four different tests (FRAP, CUPRAC, DPPH and ABTS) and obtained results were combined in unique Antioxidant Composite Index (ACI). TPC values ranged from 50.48 to 121.06 and from 38.04 to 108.17 mg GAE/g dry weight for crude and defatted grape seed extracts, respectively. Higher phenolic content determined in extracts obtained from crude seeds could be explained by the presence of lipophilic biologically active compounds in non-removed lipid fraction. Discrepancies between varieties are presumably present due to different morphological properties of seeds, environmental and agricultural conditions. Concerning antioxidant activity, all tests were significantly correlated with TPC, suggesting the strong contribution of phenolic compounds to this biological activity.", publisher = "University of Belgrade, Belgrade, Serbia", journal = "Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade", title = "Impact of defatting process on antioxidant potential of grape seeds", pages = "34-34", url = "https://hdl.handle.net/21.15107/rcub_farfar_5397" }
Dabetić, N., Todorović, V., Vidović, B.,& Šobajić, S.. (2020). Impact of defatting process on antioxidant potential of grape seeds. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade University of Belgrade, Belgrade, Serbia., 34-34. https://hdl.handle.net/21.15107/rcub_farfar_5397
Dabetić N, Todorović V, Vidović B, Šobajić S. Impact of defatting process on antioxidant potential of grape seeds. in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade. 2020;:34-34. https://hdl.handle.net/21.15107/rcub_farfar_5397 .
Dabetić, Nevena, Todorović, Vanja, Vidović, Bojana, Šobajić, Slađana, "Impact of defatting process on antioxidant potential of grape seeds" in Nutraceuticals in balancing redox status in ageing and age-related diseases WGs Meeting of the NutRedOx COST Action CA16112 Belgrade (2020):34-34, https://hdl.handle.net/21.15107/rcub_farfar_5397 .