Adsorptive stripping voltammetric determination of cefetamet in human urine

Abstract

Abstract On the basis of previously established mechanism of cefetamet (CEF) reduction, two methods were suggested for CEF determination*/differential pulse polarographic and differential pulse adsorptive stripping voltammetric method. Two pH values were chosen, 2.0 and 8.4, where the electrochemical process was defined as one four-electron and two two-electron processes, respectively. The methods were performed in Britton/Robinson (BR) buffer and the corresponding calibration graphs were constructed and statistical parameters were evaluated. Applying the AdSV method at pH 2.0 linearity was achieved from 210 8 to 210 7 M with limit detection and limit determination of 410 9 and 1.410 8 M, respectively. At pH 8.4, the linearity was obtained between 610 8 and 610 7 M, with limit detection and limit determination of 1.510 8 and 510 8 M, respectively. Since the AdSV method enabled lower concentrations of CEF to be determined, this method was tested for CEF determination in spiked urine samples, and DPP method was used as a comparative one.