Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy
Само за регистроване кориснике
2024
Аутори
Račić, AnđelkaJurišić-Dukovski, Bisera
Lovrić, Jasmina
Dobričić, Vladimir
Vučen, Sonja
Micov, Ana
Stepanović-Petrović, Radica
Tomić, Maja
Pecikoza, Uroš
Bajac, Jelena
Krajišnik, Danina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based... models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.
Кључне речи:
ketotifen hydrogen fumarate / polysaccharide polymers / rheological synergism / mucoadhesivity / HCE-T cell-based models / ocular itch testИзвор:
International Journal of Pharmaceutics, 2024, 655, 124033-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
- Project BeatDED (IP-2019-04-2174) funded by the Croatian Science Foundation.
- The authors would also like to thank the late Professor Bojan Čalija for his help with the DSC and FTIR analysis.
DOI: 10.1016/j.ijpharm.2024.124033
ISSN: 0378-5173
PubMed: 38522490
Scopus: 2-s2.0-85189766406
Институција/група
PharmacyTY - JOUR AU - Račić, Anđelka AU - Jurišić-Dukovski, Bisera AU - Lovrić, Jasmina AU - Dobričić, Vladimir AU - Vučen, Sonja AU - Micov, Ana AU - Stepanović-Petrović, Radica AU - Tomić, Maja AU - Pecikoza, Uroš AU - Bajac, Jelena AU - Krajišnik, Danina PY - 2024 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5610 AB - The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient, containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of polymers have suitable physicochemical and functional properties with demonstrated synergism between combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results, both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers for ketotifen for ophthalmic use. PB - Elsevier T2 - International Journal of Pharmaceutics T1 - Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy VL - 655 SP - 124033 DO - 10.1016/j.ijpharm.2024.124033 ER -
@article{ author = "Račić, Anđelka and Jurišić-Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Vučen, Sonja and Micov, Ana and Stepanović-Petrović, Radica and Tomić, Maja and Pecikoza, Uroš and Bajac, Jelena and Krajišnik, Danina", year = "2024", abstract = "The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient, containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of polymers have suitable physicochemical and functional properties with demonstrated synergism between combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results, both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers for ketotifen for ophthalmic use.", publisher = "Elsevier", journal = "International Journal of Pharmaceutics", title = "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy", volume = "655", pages = "124033", doi = "10.1016/j.ijpharm.2024.124033" }
Račić, A., Jurišić-Dukovski, B., Lovrić, J., Dobričić, V., Vučen, S., Micov, A., Stepanović-Petrović, R., Tomić, M., Pecikoza, U., Bajac, J.,& Krajišnik, D.. (2024). Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics Elsevier., 655, 124033. https://doi.org/10.1016/j.ijpharm.2024.124033
Račić A, Jurišić-Dukovski B, Lovrić J, Dobričić V, Vučen S, Micov A, Stepanović-Petrović R, Tomić M, Pecikoza U, Bajac J, Krajišnik D. Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics. 2024;655:124033. doi:10.1016/j.ijpharm.2024.124033 .
Račić, Anđelka, Jurišić-Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Vučen, Sonja, Micov, Ana, Stepanović-Petrović, Radica, Tomić, Maja, Pecikoza, Uroš, Bajac, Jelena, Krajišnik, Danina, "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy" in International Journal of Pharmaceutics, 655 (2024):124033, https://doi.org/10.1016/j.ijpharm.2024.124033 . .