Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
Само за регистроване кориснике
2024
Аутори
Bruić, MarijaPirković, Andrea
Borozan, Sunčica
Nacka-Aleksić, Mirjana
Jovanović-Krivokuća, Milica
Spremo-Potparević, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammato...ry activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.
Кључне речи:
taxifolin / trophoblast / oxidative stress / ROS InflammationИзвор:
Reproductive Toxicology, 2024, 126, 108585-Издавач:
- Elsevier
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200019 (Универзитет у Београду, Институт за примену нуклеарне енергије - ИНЕП) (RS-MESTD-inst-2020-200019)
DOI: 10.1016/j.reprotox.2024.108585
ISSN: 0890-6238
PubMed: 38574953
Scopus: 2-s2.0-85189469422
Институција/група
PharmacyTY - JOUR AU - Bruić, Marija AU - Pirković, Andrea AU - Borozan, Sunčica AU - Nacka-Aleksić, Mirjana AU - Jovanović-Krivokuća, Milica AU - Spremo-Potparević, Biljana PY - 2024 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5613 AB - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec- ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content, extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en- zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti- inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL- 6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti- inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po- tential as a therapeutic agent in preventing adverse pregnancy outcomes. PB - Elsevier T2 - Reproductive Toxicology T1 - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line VL - 126 SP - 108585 DO - 10.1016/j.reprotox.2024.108585 ER -
@article{ author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Jovanović-Krivokuća, Milica and Spremo-Potparević, Biljana", year = "2024", abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec- ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content, extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en- zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti- inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL- 6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti- inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po- tential as a therapeutic agent in preventing adverse pregnancy outcomes.", publisher = "Elsevier", journal = "Reproductive Toxicology", title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line", volume = "126", pages = "108585", doi = "10.1016/j.reprotox.2024.108585" }
Bruić, M., Pirković, A., Borozan, S., Nacka-Aleksić, M., Jovanović-Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology Elsevier., 126, 108585. https://doi.org/10.1016/j.reprotox.2024.108585
Bruić M, Pirković A, Borozan S, Nacka-Aleksić M, Jovanović-Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585. doi:10.1016/j.reprotox.2024.108585 .
Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Jovanović-Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585, https://doi.org/10.1016/j.reprotox.2024.108585 . .