Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research
Само за регистроване кориснике
2024
Аутори
Andrys, RudolfMonnier, Charline
Antonijević-Miljaković, Evica
![](/themes/MirageFarFaR/images/orcid.png)
Mickova, Veronika
Musilek, Kamil
![](/themes/MirageFarFaR/images/orcid.png)
Zemanova, Lucie
Чланак у часопису (Објављена верзија)
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Метаподаци
Приказ свих података о документуАпстракт
Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using par...ticular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.
Кључне речи:
Neurological disorders / Cholinesterases / Immobilization / Magnetic microparticles / Monoamine oxidases / SustainabilityИзвор:
Talanta, 2024, 276Издавач:
- Elsevier B.V.
Финансирање / пројекти:
- The Czech Science Foundation (no. GA22-14568S)
- The University of Hradec Kralove II (no. CZ.02.2.69/0.0/0.0/18_053/ 0017841)
- University of Hradec Kralove (Faculty of Science, no. SV2106-2023
- Excellence project PˇrF UHK 2201/2024–2025
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-MESTD-inst-2020-200161)
DOI: 10.1016/j.talanta.2024.126263
ISSN: 0039-9140
PubMed: 38788378
Scopus: 2-s2.0-85193620822
Институција/група
PharmacyTY - JOUR AU - Andrys, Rudolf AU - Monnier, Charline AU - Antonijević-Miljaković, Evica AU - Mickova, Veronika AU - Musilek, Kamil AU - Zemanova, Lucie PY - 2024 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5643 AB - Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery. PB - Elsevier B.V. T2 - Talanta T1 - Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research VL - 276 DO - 10.1016/j.talanta.2024.126263 ER -
@article{ author = "Andrys, Rudolf and Monnier, Charline and Antonijević-Miljaković, Evica and Mickova, Veronika and Musilek, Kamil and Zemanova, Lucie", year = "2024", abstract = "Enzyme handling and utilization bears many challenges such as their limited stability, intolerance of organic solvents, high cost, or inability to reuse. Most of these limitations can be overcome by enzyme immobilization on the surface of solid support. In this work, the recombinant form of human cholinesterases and monoamine oxidases as important drug targets for neurological diseases were immobilized on the surface of magnetic non-porous microparticles by a non-covalent bond utilizing the interaction between a His-tag terminus on the recombinant enzymes and cobalt (Co2+) ions immobilized on the magnetic microparticles. This type of binding led to targeted enzyme orientation, which completely preserved the catalytic activity and allowed high reproducibility of immobilization. In comparison with free enzymes, the immobilized enzymes showed exceptional stability in time and the possibility of repeated use. Relevant Km, Vmax, and IC50 values using known inhibitors were obtained using particular immobilized enzymes. Such immobilized enzymes on magnetic particles could serve as an excellent tool for a sustainable approach in the early stage of drug discovery.", publisher = "Elsevier B.V.", journal = "Talanta", title = "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research", volume = "276", doi = "10.1016/j.talanta.2024.126263" }
Andrys, R., Monnier, C., Antonijević-Miljaković, E., Mickova, V., Musilek, K.,& Zemanova, L.. (2024). Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta Elsevier B.V.., 276. https://doi.org/10.1016/j.talanta.2024.126263
Andrys R, Monnier C, Antonijević-Miljaković E, Mickova V, Musilek K, Zemanova L. Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research. in Talanta. 2024;276. doi:10.1016/j.talanta.2024.126263 .
Andrys, Rudolf, Monnier, Charline, Antonijević-Miljaković, Evica, Mickova, Veronika, Musilek, Kamil, Zemanova, Lucie, "Towards cost-effective drug discovery: Reusable immobilized enzymes for neurological disease research" in Talanta, 276 (2024), https://doi.org/10.1016/j.talanta.2024.126263 . .