Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations
Samo za registrovane korisnike
1990
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
A densitometric method for determination of complex mixtures of conjugated oestrogens in raw material and tablets was developed. The proposed procedure comprised hydrolysis of sodium sulphate esters of oestrone, equilin, 17α-oestradiol, equilenin, 17α-dihydroequilin and 17α-dihydroequilenin, the chloroform extraction of free oestrogens and methyltestosterone (internal standard) and separation on TLC plates using chloroform-cyclohexane-dioxane-triethyl-amine (4.5:4.1:0.6, v/v) as the solvent for development. Quantitative assay was achieved by direct scanning of the oestrogen spots at 280 nm. The proposed method is simple, rapid, reproducible and adequate to control the content of conjugated oestrogens in the raw material and in pharmaceutical preparations.
Ključne reči:
Conjugated oestrogens / densitometry / quantitative assay / raw material / sample preparation. / TLC separationIzvor:
Journal of Pharmaceutical and Biomedical Analysis, 1990, 8, 3, 253-257Institucija/grupa
PharmacyTY - JOUR AU - Novaković, J AU - Agbaba, Danica AU - Vladimirov, S AU - Živanov-Stakić, Dobrila PY - 1990 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/57 AB - A densitometric method for determination of complex mixtures of conjugated oestrogens in raw material and tablets was developed. The proposed procedure comprised hydrolysis of sodium sulphate esters of oestrone, equilin, 17α-oestradiol, equilenin, 17α-dihydroequilin and 17α-dihydroequilenin, the chloroform extraction of free oestrogens and methyltestosterone (internal standard) and separation on TLC plates using chloroform-cyclohexane-dioxane-triethyl-amine (4.5:4.1:0.6, v/v) as the solvent for development. Quantitative assay was achieved by direct scanning of the oestrogen spots at 280 nm. The proposed method is simple, rapid, reproducible and adequate to control the content of conjugated oestrogens in the raw material and in pharmaceutical preparations. T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations VL - 8 IS - 3 SP - 253 EP - 257 DO - 10.1016/0731-7085(90)80034-M ER -
@article{ author = "Novaković, J and Agbaba, Danica and Vladimirov, S and Živanov-Stakić, Dobrila", year = "1990", abstract = "A densitometric method for determination of complex mixtures of conjugated oestrogens in raw material and tablets was developed. The proposed procedure comprised hydrolysis of sodium sulphate esters of oestrone, equilin, 17α-oestradiol, equilenin, 17α-dihydroequilin and 17α-dihydroequilenin, the chloroform extraction of free oestrogens and methyltestosterone (internal standard) and separation on TLC plates using chloroform-cyclohexane-dioxane-triethyl-amine (4.5:4.1:0.6, v/v) as the solvent for development. Quantitative assay was achieved by direct scanning of the oestrogen spots at 280 nm. The proposed method is simple, rapid, reproducible and adequate to control the content of conjugated oestrogens in the raw material and in pharmaceutical preparations.", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations", volume = "8", number = "3", pages = "253-257", doi = "10.1016/0731-7085(90)80034-M" }
Novaković, J., Agbaba, D., Vladimirov, S.,& Živanov-Stakić, D.. (1990). Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis, 8(3), 253-257. https://doi.org/10.1016/0731-7085(90)80034-M
Novaković J, Agbaba D, Vladimirov S, Živanov-Stakić D. Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations. in Journal of Pharmaceutical and Biomedical Analysis. 1990;8(3):253-257. doi:10.1016/0731-7085(90)80034-M .
Novaković, J, Agbaba, Danica, Vladimirov, S, Živanov-Stakić, Dobrila, "Densitometric determination of conjugated oestrogens in the raw material and in pharmaceutical preparations" in Journal of Pharmaceutical and Biomedical Analysis, 8, no. 3 (1990):253-257, https://doi.org/10.1016/0731-7085(90)80034-M . .