In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production
Apstrakt
This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the m...edium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).
Ključne reči:
anthraquinones production / Frangula alnus / Frangula rupestris / propagation / Rhamnus catharticus / shoot culturesIzvor:
Pharmaceutical Biology, 2005, 43, 5, 420-424Izdavač:
- Taylor & Francis Ltd, Abingdon
DOI: 10.1080/13880200590963691
ISSN: 1388-0209
WoS: 000232026900006
Scopus: 2-s2.0-24744450333
Institucija/grupa
PharmacyTY - JOUR AU - Kovačević, Nada AU - Grubišić, Dragoljub PY - 2005 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/620 AB - This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)). PB - Taylor & Francis Ltd, Abingdon T2 - Pharmaceutical Biology T1 - In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production VL - 43 IS - 5 SP - 420 EP - 424 DO - 10.1080/13880200590963691 ER -
@article{ author = "Kovačević, Nada and Grubišić, Dragoljub", year = "2005", abstract = "This study was conducted to investigate the effect of physical and chemical features on metabolic production in plant cell culture. The best multiplication of Frangula alnus Mill. shoots (8.84 +/- 1.11) was obtained on woody plant medium ( WPM) medium with indole-3-acetic acid (IAA) (0.1 mg l(-1)) and 6-benzylaminapurine (BAP) (1.0 mg l(-1)), but the highest metabolite production (1731 mg/100 g of total anthraquinone (AQ) aglycones) was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg l(-1)) and thidiazuron (TDZ) (0.1 mg l(-1)). The best multiplication of Frangula rupestris (Scop.) Schur. shoots (6.20 +/- 0.53) was obtained on MS medium supplemented with BAP (0.5 mg l(-1)). The highest metabolite production (451 mg/100 g of total anthraquinone aglycones) was in the shoots grown on medium with 2,4-D (0.1 mg l(-1)) and BAP (0.5 mg l(-1)). For the multiplication of Rhamnus catharticus L. shoots, the best results (2.8 +/- 0.7) were obtained on the medium with combination of 2,4-D (0.1 mg l(-1)) and kinetine (Kin) (1.0 mg l(-1)). The best synthesis of anthraquinones (AQs) (1205 mg/100 g of total AQ aglycones) was in the shoots, which were grown on MS medium supplemented with 2,4-D (0.1 mg l(-1)).", publisher = "Taylor & Francis Ltd, Abingdon", journal = "Pharmaceutical Biology", title = "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production", volume = "43", number = "5", pages = "420-424", doi = "10.1080/13880200590963691" }
Kovačević, N.,& Grubišić, D.. (2005). In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology Taylor & Francis Ltd, Abingdon., 43(5), 420-424. https://doi.org/10.1080/13880200590963691
Kovačević N, Grubišić D. In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production. in Pharmaceutical Biology. 2005;43(5):420-424. doi:10.1080/13880200590963691 .
Kovačević, Nada, Grubišić, Dragoljub, "In vitro cultures of plants from the rhamnaceae: Shoot propagation and anthraquinones production" in Pharmaceutical Biology, 43, no. 5 (2005):420-424, https://doi.org/10.1080/13880200590963691 . .