Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography
Апстракт
Simple, rapid, and precise densitometric TLC methods have been established for simultaneous determination of loratadine and preservatives in loratadine-sodium benzoate and loratadine-methylparaben-propylparaben mixtures. Chromatography was performed on aluminium plates precoated with silica gel 60 F-254. The mobile phases were n-butyl acetate-carbon tetrachloride-acetic acid-acetonitrile 3:6:0.2:3 (v/v) for loratadine-sodium benzoate mixtures and ethyl acetate-n-hexane-methanol-ammonia-diethylamine 1:4:0.8:0.4:2 (v/v) for loratadine-methylparaben-propylparaben mixtures. Chromatographic zones were scanned in reflectance-absorbance mode at 240 nm for the former mixture and 275 mu for the latter. Relationships between peak areas and amounts of the compounds were evaluated by linear regression analysis in the concentration ranges 0.3-0.7 mu g per band for loratadine, methylparaben, and sodium benzoate and 0.03-0.07 mu g per band for propylparaben. Mean recovery for all the compounds varied... from 98.3 to 102.5 %. Detection and quantification limits ranged from 0.004 to 0.03 and from 0.01 to 0.1 mu g per band, respectively. The proposed method was used for simultaneous determination of loratadine. and the preservatives in commercial medicinal syrups.
Извор:
Acta Chromatographica, 2007, 19, 161-169Издавач:
- Univ Silesia, Inst Chemistry, Katowice
Финансирање / пројекти:
- Структура, термодинамичке и електрохемијске особине материјала за конверзију енергије и нове технологије (RS-MESTD-MPN2006-2010-142047)
- Супстанце за фармацеутску употребу: моделирање, синтеза, физичко-хемијске и биолошке особине, степен чистоће и испитивање дозираних облика (RS-MESTD-MPN2006-2010-142072)
Институција/група
PharmacyTY - JOUR AU - Popović, Gordana AU - Čakar, Mira AU - Agbaba, Danica PY - 2007 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/905 AB - Simple, rapid, and precise densitometric TLC methods have been established for simultaneous determination of loratadine and preservatives in loratadine-sodium benzoate and loratadine-methylparaben-propylparaben mixtures. Chromatography was performed on aluminium plates precoated with silica gel 60 F-254. The mobile phases were n-butyl acetate-carbon tetrachloride-acetic acid-acetonitrile 3:6:0.2:3 (v/v) for loratadine-sodium benzoate mixtures and ethyl acetate-n-hexane-methanol-ammonia-diethylamine 1:4:0.8:0.4:2 (v/v) for loratadine-methylparaben-propylparaben mixtures. Chromatographic zones were scanned in reflectance-absorbance mode at 240 nm for the former mixture and 275 mu for the latter. Relationships between peak areas and amounts of the compounds were evaluated by linear regression analysis in the concentration ranges 0.3-0.7 mu g per band for loratadine, methylparaben, and sodium benzoate and 0.03-0.07 mu g per band for propylparaben. Mean recovery for all the compounds varied from 98.3 to 102.5 %. Detection and quantification limits ranged from 0.004 to 0.03 and from 0.01 to 0.1 mu g per band, respectively. The proposed method was used for simultaneous determination of loratadine. and the preservatives in commercial medicinal syrups. PB - Univ Silesia, Inst Chemistry, Katowice T2 - Acta Chromatographica T1 - Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography VL - 19 SP - 161 EP - 169 UR - https://hdl.handle.net/21.15107/rcub_farfar_905 ER -
@article{ author = "Popović, Gordana and Čakar, Mira and Agbaba, Danica", year = "2007", abstract = "Simple, rapid, and precise densitometric TLC methods have been established for simultaneous determination of loratadine and preservatives in loratadine-sodium benzoate and loratadine-methylparaben-propylparaben mixtures. Chromatography was performed on aluminium plates precoated with silica gel 60 F-254. The mobile phases were n-butyl acetate-carbon tetrachloride-acetic acid-acetonitrile 3:6:0.2:3 (v/v) for loratadine-sodium benzoate mixtures and ethyl acetate-n-hexane-methanol-ammonia-diethylamine 1:4:0.8:0.4:2 (v/v) for loratadine-methylparaben-propylparaben mixtures. Chromatographic zones were scanned in reflectance-absorbance mode at 240 nm for the former mixture and 275 mu for the latter. Relationships between peak areas and amounts of the compounds were evaluated by linear regression analysis in the concentration ranges 0.3-0.7 mu g per band for loratadine, methylparaben, and sodium benzoate and 0.03-0.07 mu g per band for propylparaben. Mean recovery for all the compounds varied from 98.3 to 102.5 %. Detection and quantification limits ranged from 0.004 to 0.03 and from 0.01 to 0.1 mu g per band, respectively. The proposed method was used for simultaneous determination of loratadine. and the preservatives in commercial medicinal syrups.", publisher = "Univ Silesia, Inst Chemistry, Katowice", journal = "Acta Chromatographica", title = "Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography", volume = "19", pages = "161-169", url = "https://hdl.handle.net/21.15107/rcub_farfar_905" }
Popović, G., Čakar, M.,& Agbaba, D.. (2007). Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography. in Acta Chromatographica Univ Silesia, Inst Chemistry, Katowice., 19, 161-169. https://hdl.handle.net/21.15107/rcub_farfar_905
Popović G, Čakar M, Agbaba D. Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography. in Acta Chromatographica. 2007;19:161-169. https://hdl.handle.net/21.15107/rcub_farfar_905 .
Popović, Gordana, Čakar, Mira, Agbaba, Danica, "Simultaneous determination of loratadine and preservatives in syrups by thin-layer chromatography" in Acta Chromatographica, 19 (2007):161-169, https://hdl.handle.net/21.15107/rcub_farfar_905 .