The role of nitric oxide in paraquat-induced oxidative stress in rat striatum
Аутори
Đukić, MirjanaĆurčić-Jovanović, Marijana
Ninković, Milica
Vasijević, Ivana
Jovanović, Marina
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The role of nitric oxide (NO) in paraquat (PQ)-induced neurotoxicity is still not fully understood. In this study we used N-G-nitro-L-arginine methyl ester (L-NAME), a non-selective nitric oxide synthase (NOS) inhibitor, in order to examine the effects of NO, reactive oxygen species (ROS) generation and lipid peroxidation (LPO) development during PQ-mediated neurotoxicity. Oxidative stress development in the striatum of Wistar rats intrastriatally (i.s.) poisoned with PQ (and in some cases pre-treated with L-NAME) was investigated by measuring superoxide anion (O-2(center dot-)), malondialdehyde (MDA) and nitrate (NO3-), 30 min, 24 hours and 7 days after treatment. L-NAME pretreatment provided the possibility to distinguish the role of ROS from reactive nitrogen species (RNS) in oxidative stress development induced by PQ. Our results confirm the involvement of NO in PQ-mediated neurotoxicity and reduced LPO by L-NAME pretreatment implying that the latter has a protective role.
Кључне речи:
paraquat / oxidative stress / nitric oxide / L-NAME / Wistar rats / brainИзвор:
Annals of Agricultural and Environmental Medicine, 2007, 14, 2, 247-252Издавач:
- Inst Agricultural Medicine, Lublin
Финансирање / пројекти:
- Биохемијски показатељи оштећења и дисфункције органа (RS-MESTD-MPN2006-2010-145010)
Институција/група
PharmacyTY - JOUR AU - Đukić, Mirjana AU - Ćurčić-Jovanović, Marijana AU - Ninković, Milica AU - Vasijević, Ivana AU - Jovanović, Marina PY - 2007 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/908 AB - The role of nitric oxide (NO) in paraquat (PQ)-induced neurotoxicity is still not fully understood. In this study we used N-G-nitro-L-arginine methyl ester (L-NAME), a non-selective nitric oxide synthase (NOS) inhibitor, in order to examine the effects of NO, reactive oxygen species (ROS) generation and lipid peroxidation (LPO) development during PQ-mediated neurotoxicity. Oxidative stress development in the striatum of Wistar rats intrastriatally (i.s.) poisoned with PQ (and in some cases pre-treated with L-NAME) was investigated by measuring superoxide anion (O-2(center dot-)), malondialdehyde (MDA) and nitrate (NO3-), 30 min, 24 hours and 7 days after treatment. L-NAME pretreatment provided the possibility to distinguish the role of ROS from reactive nitrogen species (RNS) in oxidative stress development induced by PQ. Our results confirm the involvement of NO in PQ-mediated neurotoxicity and reduced LPO by L-NAME pretreatment implying that the latter has a protective role. PB - Inst Agricultural Medicine, Lublin T2 - Annals of Agricultural and Environmental Medicine T1 - The role of nitric oxide in paraquat-induced oxidative stress in rat striatum VL - 14 IS - 2 SP - 247 EP - 252 UR - https://hdl.handle.net/21.15107/rcub_farfar_908 ER -
@article{ author = "Đukić, Mirjana and Ćurčić-Jovanović, Marijana and Ninković, Milica and Vasijević, Ivana and Jovanović, Marina", year = "2007", abstract = "The role of nitric oxide (NO) in paraquat (PQ)-induced neurotoxicity is still not fully understood. In this study we used N-G-nitro-L-arginine methyl ester (L-NAME), a non-selective nitric oxide synthase (NOS) inhibitor, in order to examine the effects of NO, reactive oxygen species (ROS) generation and lipid peroxidation (LPO) development during PQ-mediated neurotoxicity. Oxidative stress development in the striatum of Wistar rats intrastriatally (i.s.) poisoned with PQ (and in some cases pre-treated with L-NAME) was investigated by measuring superoxide anion (O-2(center dot-)), malondialdehyde (MDA) and nitrate (NO3-), 30 min, 24 hours and 7 days after treatment. L-NAME pretreatment provided the possibility to distinguish the role of ROS from reactive nitrogen species (RNS) in oxidative stress development induced by PQ. Our results confirm the involvement of NO in PQ-mediated neurotoxicity and reduced LPO by L-NAME pretreatment implying that the latter has a protective role.", publisher = "Inst Agricultural Medicine, Lublin", journal = "Annals of Agricultural and Environmental Medicine", title = "The role of nitric oxide in paraquat-induced oxidative stress in rat striatum", volume = "14", number = "2", pages = "247-252", url = "https://hdl.handle.net/21.15107/rcub_farfar_908" }
Đukić, M., Ćurčić-Jovanović, M., Ninković, M., Vasijević, I.,& Jovanović, M.. (2007). The role of nitric oxide in paraquat-induced oxidative stress in rat striatum. in Annals of Agricultural and Environmental Medicine Inst Agricultural Medicine, Lublin., 14(2), 247-252. https://hdl.handle.net/21.15107/rcub_farfar_908
Đukić M, Ćurčić-Jovanović M, Ninković M, Vasijević I, Jovanović M. The role of nitric oxide in paraquat-induced oxidative stress in rat striatum. in Annals of Agricultural and Environmental Medicine. 2007;14(2):247-252. https://hdl.handle.net/21.15107/rcub_farfar_908 .
Đukić, Mirjana, Ćurčić-Jovanović, Marijana, Ninković, Milica, Vasijević, Ivana, Jovanović, Marina, "The role of nitric oxide in paraquat-induced oxidative stress in rat striatum" in Annals of Agricultural and Environmental Medicine, 14, no. 2 (2007):247-252, https://hdl.handle.net/21.15107/rcub_farfar_908 .