High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A selective, sensitive, and simple high-performance liquid chromatographic (HPLC) method was developed for the determination of moclobemide and its two major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma. Sample preparation (0.5 ml of plasma) involved solid-phase extraction (SPE) using Speedisk (R) H2O-Philic DVB columns. Separations were performed on a Waters XTerra (TM) RP18 column (5 mu m, 150 mm x 4.6 mm). The mobile phase consisted of 10 mM KH2PO4 with 1% triethylamine (pH 3.9) and acetonitrile (93:17, v/v), and a flow-rate was 1.2 ml/min. The total run time was 13 min. UV detection was performed at 240 nm. Mean absolute recoveries were >= 90% and the limit of quantification (LOQ) for all analytes was 0.02 mg/l. Calibration curves were linear (r > 0.995) over a wide range of the analyte concentrations in plasma; thus, the method is suitable for different clinical studies when large variations in the drug/metabolites concentrations are observed. During a 5-day assay valid...ation procedure the accuracy and precision were tested and proven (relative errors (RE) lt = 13%; intra-day coefficient of variation (CV) lt = 7%; inter-day CV lt = 13%). Many drugs frequently used in the target patient population were evaluated for potential interference in order method selectivity to be ensured. The assay has been used in a clinical pharmacokinetic study to assess steady-state pharmacokinetics of moclobenide and two metabolites in depressive patients on mono- and combined therapy.
Кључне речи:
moclobemide / metabolites / plasma / HPLC / solid-phase extractionИзвор:
Journal of Pharmaceutical and Biomedical Analysis, 2007, 43, 4, 1416-1422Издавач:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/j.jpba.2006.10.032
ISSN: 0731-7085
PubMed: 17141442
WoS: 000245262300031
Scopus: 2-s2.0-33847237604
Институција/група
PharmacyTY - JOUR AU - Rakić, Anita AU - Miljković, Branislava AU - Pokrajac, Milena AU - Vučićević, Katarina PY - 2007 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/948 AB - A selective, sensitive, and simple high-performance liquid chromatographic (HPLC) method was developed for the determination of moclobemide and its two major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma. Sample preparation (0.5 ml of plasma) involved solid-phase extraction (SPE) using Speedisk (R) H2O-Philic DVB columns. Separations were performed on a Waters XTerra (TM) RP18 column (5 mu m, 150 mm x 4.6 mm). The mobile phase consisted of 10 mM KH2PO4 with 1% triethylamine (pH 3.9) and acetonitrile (93:17, v/v), and a flow-rate was 1.2 ml/min. The total run time was 13 min. UV detection was performed at 240 nm. Mean absolute recoveries were >= 90% and the limit of quantification (LOQ) for all analytes was 0.02 mg/l. Calibration curves were linear (r > 0.995) over a wide range of the analyte concentrations in plasma; thus, the method is suitable for different clinical studies when large variations in the drug/metabolites concentrations are observed. During a 5-day assay validation procedure the accuracy and precision were tested and proven (relative errors (RE) lt = 13%; intra-day coefficient of variation (CV) lt = 7%; inter-day CV lt = 13%). Many drugs frequently used in the target patient population were evaluated for potential interference in order method selectivity to be ensured. The assay has been used in a clinical pharmacokinetic study to assess steady-state pharmacokinetics of moclobenide and two metabolites in depressive patients on mono- and combined therapy. PB - Elsevier Science BV, Amsterdam T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma VL - 43 IS - 4 SP - 1416 EP - 1422 DO - 10.1016/j.jpba.2006.10.032 ER -
@article{ author = "Rakić, Anita and Miljković, Branislava and Pokrajac, Milena and Vučićević, Katarina", year = "2007", abstract = "A selective, sensitive, and simple high-performance liquid chromatographic (HPLC) method was developed for the determination of moclobemide and its two major metabolites, Ro 12-5637 and Ro 12-8095, in human plasma. Sample preparation (0.5 ml of plasma) involved solid-phase extraction (SPE) using Speedisk (R) H2O-Philic DVB columns. Separations were performed on a Waters XTerra (TM) RP18 column (5 mu m, 150 mm x 4.6 mm). The mobile phase consisted of 10 mM KH2PO4 with 1% triethylamine (pH 3.9) and acetonitrile (93:17, v/v), and a flow-rate was 1.2 ml/min. The total run time was 13 min. UV detection was performed at 240 nm. Mean absolute recoveries were >= 90% and the limit of quantification (LOQ) for all analytes was 0.02 mg/l. Calibration curves were linear (r > 0.995) over a wide range of the analyte concentrations in plasma; thus, the method is suitable for different clinical studies when large variations in the drug/metabolites concentrations are observed. During a 5-day assay validation procedure the accuracy and precision were tested and proven (relative errors (RE) lt = 13%; intra-day coefficient of variation (CV) lt = 7%; inter-day CV lt = 13%). Many drugs frequently used in the target patient population were evaluated for potential interference in order method selectivity to be ensured. The assay has been used in a clinical pharmacokinetic study to assess steady-state pharmacokinetics of moclobenide and two metabolites in depressive patients on mono- and combined therapy.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma", volume = "43", number = "4", pages = "1416-1422", doi = "10.1016/j.jpba.2006.10.032" }
Rakić, A., Miljković, B., Pokrajac, M.,& Vučićević, K.. (2007). High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma. in Journal of Pharmaceutical and Biomedical Analysis Elsevier Science BV, Amsterdam., 43(4), 1416-1422. https://doi.org/10.1016/j.jpba.2006.10.032
Rakić A, Miljković B, Pokrajac M, Vučićević K. High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma. in Journal of Pharmaceutical and Biomedical Analysis. 2007;43(4):1416-1422. doi:10.1016/j.jpba.2006.10.032 .
Rakić, Anita, Miljković, Branislava, Pokrajac, Milena, Vučićević, Katarina, "High-performance liquid chromatographic method for the determination of moclobemide and its two major metabolites in human plasma" in Journal of Pharmaceutical and Biomedical Analysis, 43, no. 4 (2007):1416-1422, https://doi.org/10.1016/j.jpba.2006.10.032 . .