LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures
Само за регистроване кориснике
2008
Аутори
Popović, IgorIvanović, Darko
Medenica, Mirjana
Malenović, Anđelija
Jančić-Stojanović, Biljana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The main objective in all optimization procedures is to define the most appropriate conditions for rapid, sensitive, precise, and reproducible analysis, as economically as possible. Experimental design and DryLab optimization software have been used to optimize a liquid chromatographic method for separation of lercanidipine and its three impurities. In both methods of optimization the acetonitrile content and pH of the mobile phase were factors extracted for analysis; resolution of a critical pair was output in both cases. Data obtained from both optimization methods were compared and appropriate conclusions were extracted with the objective of gaining a complete view of chromatographic behavior. Detailed description was obtained by use of a three-dimensional graph and DryLab maps. Both methods of optimization reported the most appropriate mobile phase to be a 35:65 mixture of acetonitrile and an aqueous solution of 1.5% TEA, pH adjusted to 3.0 by addition of orthophosphoric acid, at a... flow rate 1.0 mL min(-1). Separations were performed on a 20 x 4.6 mm, 3.5-mu m particle size, C-18 column, at 20 degrees C, with UV detection at 240 nm. The method was validated. All the results proved the reliability of the method, so it can be used for separation, identification, and simultaneous determination of these substances in the drug and in pharmaceutical dosage forms.
Кључне речи:
column liquid chromatography / optimization / experimental design / DryLab software / lercanidipine hydrochloride / impurities / validationИзвор:
Chromatographia, 2008, 67, 5-6, 449-454Издавач:
- Vieweg, Wiesbaden
Финансирање / пројекти:
- Формулисање и Карактеризација сепарационих система за моделовање ретенционог понашања лековитих супстанција уз хемометријску евалуацију (RS-MESTD-MPN2006-2010-142077)
DOI: 10.1365/s10337-008-0536-3
ISSN: 0009-5893
WoS: 000253624100015
Scopus: 2-s2.0-40149108426
Институција/група
PharmacyTY - JOUR AU - Popović, Igor AU - Ivanović, Darko AU - Medenica, Mirjana AU - Malenović, Anđelija AU - Jančić-Stojanović, Biljana PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1039 AB - The main objective in all optimization procedures is to define the most appropriate conditions for rapid, sensitive, precise, and reproducible analysis, as economically as possible. Experimental design and DryLab optimization software have been used to optimize a liquid chromatographic method for separation of lercanidipine and its three impurities. In both methods of optimization the acetonitrile content and pH of the mobile phase were factors extracted for analysis; resolution of a critical pair was output in both cases. Data obtained from both optimization methods were compared and appropriate conclusions were extracted with the objective of gaining a complete view of chromatographic behavior. Detailed description was obtained by use of a three-dimensional graph and DryLab maps. Both methods of optimization reported the most appropriate mobile phase to be a 35:65 mixture of acetonitrile and an aqueous solution of 1.5% TEA, pH adjusted to 3.0 by addition of orthophosphoric acid, at a flow rate 1.0 mL min(-1). Separations were performed on a 20 x 4.6 mm, 3.5-mu m particle size, C-18 column, at 20 degrees C, with UV detection at 240 nm. The method was validated. All the results proved the reliability of the method, so it can be used for separation, identification, and simultaneous determination of these substances in the drug and in pharmaceutical dosage forms. PB - Vieweg, Wiesbaden T2 - Chromatographia T1 - LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures VL - 67 IS - 5-6 SP - 449 EP - 454 DO - 10.1365/s10337-008-0536-3 ER -
@article{ author = "Popović, Igor and Ivanović, Darko and Medenica, Mirjana and Malenović, Anđelija and Jančić-Stojanović, Biljana", year = "2008", abstract = "The main objective in all optimization procedures is to define the most appropriate conditions for rapid, sensitive, precise, and reproducible analysis, as economically as possible. Experimental design and DryLab optimization software have been used to optimize a liquid chromatographic method for separation of lercanidipine and its three impurities. In both methods of optimization the acetonitrile content and pH of the mobile phase were factors extracted for analysis; resolution of a critical pair was output in both cases. Data obtained from both optimization methods were compared and appropriate conclusions were extracted with the objective of gaining a complete view of chromatographic behavior. Detailed description was obtained by use of a three-dimensional graph and DryLab maps. Both methods of optimization reported the most appropriate mobile phase to be a 35:65 mixture of acetonitrile and an aqueous solution of 1.5% TEA, pH adjusted to 3.0 by addition of orthophosphoric acid, at a flow rate 1.0 mL min(-1). Separations were performed on a 20 x 4.6 mm, 3.5-mu m particle size, C-18 column, at 20 degrees C, with UV detection at 240 nm. The method was validated. All the results proved the reliability of the method, so it can be used for separation, identification, and simultaneous determination of these substances in the drug and in pharmaceutical dosage forms.", publisher = "Vieweg, Wiesbaden", journal = "Chromatographia", title = "LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures", volume = "67", number = "5-6", pages = "449-454", doi = "10.1365/s10337-008-0536-3" }
Popović, I., Ivanović, D., Medenica, M., Malenović, A.,& Jančić-Stojanović, B.. (2008). LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures. in Chromatographia Vieweg, Wiesbaden., 67(5-6), 449-454. https://doi.org/10.1365/s10337-008-0536-3
Popović I, Ivanović D, Medenica M, Malenović A, Jančić-Stojanović B. LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures. in Chromatographia. 2008;67(5-6):449-454. doi:10.1365/s10337-008-0536-3 .
Popović, Igor, Ivanović, Darko, Medenica, Mirjana, Malenović, Anđelija, Jančić-Stojanović, Biljana, "LC determination of lercanidipine and its impurities using DryLab software and experimental design procedures" in Chromatographia, 67, no. 5-6 (2008):449-454, https://doi.org/10.1365/s10337-008-0536-3 . .