A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol
2008
Authors
Gojković-Bukarica, LjiljanaNovaković, Aleksandra
Kanjuh, Vladimir
Bumbasirević, Marko
Lesić, Aleksandar
Heinle, Helmut
Article (Published version)
Metadata
Show full item recordAbstract
Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence... of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol.
Keywords:
resveratrol / rat mesenteric artery / ion channel / vasorelaxation / K+ channelSource:
Journal of Pharmacological Sciences, 2008, 108, 1, 124-130Publisher:
- Japanese Pharmacological Soc, Kyoto
Funding / projects:
- Ministry of Science and Technology (Serbia)
- Karl & Lora Klein Foundation (Germany)
DOI: 10.1254/jphs.08128FP
ISSN: 1347-8613
PubMed: 18818483
WoS: 000259683400015
Scopus: 2-s2.0-52949145570
Collections
Institution/Community
PharmacyTY - JOUR AU - Gojković-Bukarica, Ljiljana AU - Novaković, Aleksandra AU - Kanjuh, Vladimir AU - Bumbasirević, Marko AU - Lesić, Aleksandar AU - Heinle, Helmut PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1041 AB - Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol. PB - Japanese Pharmacological Soc, Kyoto T2 - Journal of Pharmacological Sciences T1 - A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol VL - 108 IS - 1 SP - 124 EP - 130 DO - 10.1254/jphs.08128FP ER -
@article{ author = "Gojković-Bukarica, Ljiljana and Novaković, Aleksandra and Kanjuh, Vladimir and Bumbasirević, Marko and Lesić, Aleksandar and Heinle, Helmut", year = "2008", abstract = "Recently it has been suggested that resveratrol relaxes different isolated arteries. The present study addressed the question whether different ion channels are involved in the endothelium-independent mechanism of vasodilatation induced by resveratrol. For that purpose, we tested the action of resveratrol on the rat mesenteric artery without endothelium. Resveratrol induced con centration-dependent relaxation of rat mesenteric artery. Among the K+-channel blockers, 4-amynopiridine (4-AP) moderately antagonized the resveratrol-induced relaxation, while glibendamide, tetraethylammonium chloride, charybdotoxin, margatoxin, and barium chloride did not inhibit resveratrol-induced vasorelaxation. In rings, precontracted with 100 mM K+, the relaxant responses to resveratrol were highly significantly shifted to the right compared to those obtained in rings precontracted with phenylephrine, but resveratrol-induced maximal relaxation was only slightly affected. In order to minimize the influence of K+ channels and voltage-gated Ca2+ channels (VGCCs) in vascular smooth muscle, the third contraction was made by 100 in M K- in the presence of nifedipine. The relaxant response to resveratrol was abolished. Thus, the mechanism of vasorelaxation induced by resveratrol probably involves activation of 4-AP-sensitive K+ channels. Its ability to completely relax the mesenteric artery precontracted with K+-rich solution suggests that K channel-independent mechanism(s) are involved in its vasorelaxant effect. It seems that interaction with VGCCs plays a part in this K+ channel-independent effect of resveratrol.", publisher = "Japanese Pharmacological Soc, Kyoto", journal = "Journal of Pharmacological Sciences", title = "A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol", volume = "108", number = "1", pages = "124-130", doi = "10.1254/jphs.08128FP" }
Gojković-Bukarica, L., Novaković, A., Kanjuh, V., Bumbasirević, M., Lesić, A.,& Heinle, H.. (2008). A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol. in Journal of Pharmacological Sciences Japanese Pharmacological Soc, Kyoto., 108(1), 124-130. https://doi.org/10.1254/jphs.08128FP
Gojković-Bukarica L, Novaković A, Kanjuh V, Bumbasirević M, Lesić A, Heinle H. A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol. in Journal of Pharmacological Sciences. 2008;108(1):124-130. doi:10.1254/jphs.08128FP .
Gojković-Bukarica, Ljiljana, Novaković, Aleksandra, Kanjuh, Vladimir, Bumbasirević, Marko, Lesić, Aleksandar, Heinle, Helmut, "A role of ion channels in the endothelium-independent relaxation of rat mesenteric artery induced by resveratrol" in Journal of Pharmacological Sciences, 108, no. 1 (2008):124-130, https://doi.org/10.1254/jphs.08128FP . .