Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories
Authorized Users Only
2010
Authors
Jančić-Stojanović, BiljanaMalenović, Anđelija
Marković, Slavko
Ivanović, Darko
Medenica, Mirjana
Article (Published version)
Metadata
Show full item recordAbstract
An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.
Source:
Journal of AOAC International, 2010, 93, 1, 102-107Publisher:
- AOAC Int, Gaithersburg
Funding / projects:
- Formulisanje i Karakterizacija separacionih sistema za modelovanje retencionog ponašanja lekovitih supstancija uz hemometrijsku evaluaciju (RS-MESTD-MPN2006-2010-142077)
DOI: 10.1093/jaoac/93.1.102
ISSN: 1060-3271
PubMed: 20334171
WoS: 000275096600015
Scopus: 2-s2.0-77949987112
Collections
Institution/Community
PharmacyTY - JOUR AU - Jančić-Stojanović, Biljana AU - Malenović, Anđelija AU - Marković, Slavko AU - Ivanović, Darko AU - Medenica, Mirjana PY - 2010 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1371 AB - An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness. PB - AOAC Int, Gaithersburg T2 - Journal of AOAC International T1 - Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories VL - 93 IS - 1 SP - 102 EP - 107 DO - 10.1093/jaoac/93.1.102 UR - https://hdl.handle.net/21.15107/rcub_farfar_1371 ER -
@article{ author = "Jančić-Stojanović, Biljana and Malenović, Anđelija and Marković, Slavko and Ivanović, Darko and Medenica, Mirjana", year = "2010", abstract = "An RP-HPLC method has been optimized and validated for the simultaneous determination of hydrocortisone acetate and of lidocaine in suppositories. For the method optimization, response surface methodology was applied, and the obtained model was tested using analysis of variance. The optimal separations were conducted on a Beckman-Coulter 150 x 4.6 mm, 5 pm particle-size column at 20 degrees C. The mobile phase was methanol-water (65 + 35, v/v), pH adjusted to 2.5 with 85% orthophosphoric acid, with a flow rate of 1.0 mL/min. UV detection was performed at 250 nm. Phenobarbital was used as an internal standard. The method was validated for selectivity, linearity, precision, and robustness.", publisher = "AOAC Int, Gaithersburg", journal = "Journal of AOAC International", title = "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories", volume = "93", number = "1", pages = "102-107", doi = "10.1093/jaoac/93.1.102", url = "https://hdl.handle.net/21.15107/rcub_farfar_1371" }
Jančić-Stojanović, B., Malenović, A., Marković, S., Ivanović, D.,& Medenica, M.. (2010). Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International AOAC Int, Gaithersburg., 93(1), 102-107. https://doi.org/10.1093/jaoac/93.1.102 https://hdl.handle.net/21.15107/rcub_farfar_1371
Jančić-Stojanović B, Malenović A, Marković S, Ivanović D, Medenica M. Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories. in Journal of AOAC International. 2010;93(1):102-107. doi:10.1093/jaoac/93.1.102 https://hdl.handle.net/21.15107/rcub_farfar_1371 .
Jančić-Stojanović, Biljana, Malenović, Anđelija, Marković, Slavko, Ivanović, Darko, Medenica, Mirjana, "Optimization and Validation of an RP-HPLC Method for Analysis of Hydrocortisone Acetate and Lidocaine in Suppositories" in Journal of AOAC International, 93, no. 1 (2010):102-107, https://doi.org/10.1093/jaoac/93.1.102 ., https://hdl.handle.net/21.15107/rcub_farfar_1371 .