Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations
2012
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Članak u časopisu (Objavljena verzija)
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Prikaz svih podataka o dokumentuApstrakt
A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause prob...lem in identification and measurements, hesperidin has been determined successfully.
Ključne reči:
Direct spectrophotometric method / flavonoids / hesperidin / drug analysisIzvor:
Acta Chimica Slovenica, 2012, 59, 2, 436-441Izdavač:
- Slovensko Kemijsko Drustvo, Ljubljana
Finansiranje / projekti:
- Razvoj molekula sa antiinflamatornim i kardioproaktivnim dejstvom: strukturne modifikacije, modelovanje, fizičkohemijska karakterizacija i formulaciona ispitivanja (RS-MESTD-Basic Research (BR or ON)-172041)
Institucija/grupa
PharmacyTY - JOUR AU - Kuntić, Vesna AU - Pejić, Nataša AU - Micić, Svetlana PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1734 AB - A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully. PB - Slovensko Kemijsko Drustvo, Ljubljana T2 - Acta Chimica Slovenica T1 - Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations VL - 59 IS - 2 SP - 436 EP - 441 UR - https://hdl.handle.net/21.15107/rcub_farfar_1734 ER -
@article{ author = "Kuntić, Vesna and Pejić, Nataša and Micić, Svetlana", year = "2012", abstract = "A simple, rapid and reliable direct spectrophotometric method for the determination of hesperidin is proposed and validated. The influence of wavelength, solvent, the ionic strength, pH and temperature on hesperidin determination were investigated. Under the optimum conditions, lambda = 283 nm, 60% methanol as the solvent, ionic strength, I = 2.5 x 10(-5) mol L-1, pH = 6.4 and T = 37.0 degrees C, the Beer's law is obeyed in the concentration range 1.83-24.5 mu g mL(-1). The molar absorptivity and Sandells sensitivity were found to be 1.8 x 10(4) L mol(-1) cm(-1) and 0.03 mu g cm(-2), respectively. The sensitivity of the proposed method was 0.9 mu g mL(-1) (as limit of detection) and 3.2 mu g mL(-1) (as limit of quantification). Applicability of the proposed method to the direct determination of total flavonoids as hesperidin equivalents in pharmaceutical formulation (Vitamin C with citrus bioflavonoids & Rose Hips) was demonstrated. Although the presence of ascorbic acid may cause problem in identification and measurements, hesperidin has been determined successfully.", publisher = "Slovensko Kemijsko Drustvo, Ljubljana", journal = "Acta Chimica Slovenica", title = "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations", volume = "59", number = "2", pages = "436-441", url = "https://hdl.handle.net/21.15107/rcub_farfar_1734" }
Kuntić, V., Pejić, N.,& Micić, S.. (2012). Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica Slovensko Kemijsko Drustvo, Ljubljana., 59(2), 436-441. https://hdl.handle.net/21.15107/rcub_farfar_1734
Kuntić V, Pejić N, Micić S. Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations. in Acta Chimica Slovenica. 2012;59(2):436-441. https://hdl.handle.net/21.15107/rcub_farfar_1734 .
Kuntić, Vesna, Pejić, Nataša, Micić, Svetlana, "Direct Spectrophotometric Determination of Hesperidin in Pharmaceutical Preparations" in Acta Chimica Slovenica, 59, no. 2 (2012):436-441, https://hdl.handle.net/21.15107/rcub_farfar_1734 .