Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats
Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova
2012
Autori
Đukić, MirjanaJovanović, Marina
Ninković, Milica
Stevanović, Ivana
Đurđević, Dragan
Vasić, Una
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivati...on of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days).
U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprote...ktivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.
Ključne reči:
diquat / free radicals / glutathione reductase / lipid peroxidation / neurotoxicity / nitrosative stress / oxidative stressIzvor:
Acta veterinaria, 2012, 62, 5-6, 553-568Izdavač:
- Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
Finansiranje / projekti:
DOI: 10.2298/AVB1206553D
ISSN: 0567-8315
WoS: 000313151700007
Scopus: 2-s2.0-84873933464
Institucija/grupa
PharmacyTY - JOUR AU - Đukić, Mirjana AU - Jovanović, Marina AU - Ninković, Milica AU - Stevanović, Ivana AU - Đurđević, Dragan AU - Vasić, Una PY - 2012 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1792 AB - In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days). AB - U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD. PB - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd T2 - Acta veterinaria T1 - Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats T1 - Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova VL - 62 IS - 5-6 SP - 553 EP - 568 DO - 10.2298/AVB1206553D ER -
@article{ author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Vasić, Una", year = "2012", abstract = "In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days)., U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.", publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd", journal = "Acta veterinaria", title = "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats, Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova", volume = "62", number = "5-6", pages = "553-568", doi = "10.2298/AVB1206553D" }
Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Đurđević, D.,& Vasić, U.. (2012). Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 553-568. https://doi.org/10.2298/AVB1206553D
Đukić M, Jovanović M, Ninković M, Stevanović I, Đurđević D, Vasić U. Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria. 2012;62(5-6):553-568. doi:10.2298/AVB1206553D .
Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Vasić, Una, "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats" in Acta veterinaria, 62, no. 5-6 (2012):553-568, https://doi.org/10.2298/AVB1206553D . .