DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division
Samo za registrovane korisnike
2013
Autori
Živković, LadaPotparević, Biljana
Siedlak, Sandra L.
Perry, George
Plećaš-Solarović, Bosiljka
Milicević, Zorana
Bajić, Vladan
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%..., p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright
Ključne reči:
DNA damage / Premature centromere division / Sporadic Alzheimer disease / Peripheral blood lymphocytesIzvor:
Neurodegenerative Diseases, 2013, 12, 3, 156-163Izdavač:
- Karger, Basel
Finansiranje / projekti:
- Aberacije ćelijskog ciklusa i uticaj oksidativnog stresa na neurodegenerativne procese i malignu transformaciju ćelije (RS-MESTD-Basic Research (BR or ON)-173034)
DOI: 10.1159/000346114
ISSN: 1660-2854
PubMed: 23406622
WoS: 000324448800005
Scopus: 2-s2.0-84884289680
Institucija/grupa
PharmacyTY - JOUR AU - Živković, Lada AU - Potparević, Biljana AU - Siedlak, Sandra L. AU - Perry, George AU - Plećaš-Solarović, Bosiljka AU - Milicević, Zorana AU - Bajić, Vladan PY - 2013 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1861 AB - While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright PB - Karger, Basel T2 - Neurodegenerative Diseases T1 - DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division VL - 12 IS - 3 SP - 156 EP - 163 DO - 10.1159/000346114 ER -
@article{ author = "Živković, Lada and Potparević, Biljana and Siedlak, Sandra L. and Perry, George and Plećaš-Solarović, Bosiljka and Milicević, Zorana and Bajić, Vladan", year = "2013", abstract = "While Alzheimer disease (AD) is considered a neurodegenerative disorder, the importance of chromosome instability in non-neuronal cells is equally important, not only for shedding light on the etiology of the disease, but also for possible diagnostic purposes and monitoring the progress of the disease. Here, we evaluated the frequency of DNA damage and expression of premature centromere division (PCD) in peripheral blood lymphocytes of sporadic AD patients, age-matched and young controls. The results show that in male patients with AD, the frequencies of PCD and DNA damage were significantly greater (88%, p lt 0.01 and 38%, p lt 0.05, respectively) than in age-matched control group. AD females had significantly increased frequency of PCD (134%, p lt 0.01) as well as a higher frequency of DNA damage (37%, p lt 0.05). Ageing per se, both in males and females, shows significant increase of percentages of PCD (2.3 times, p lt 0.01 and 2.8 times, p lt 0.01, respectively) and DNA damage (63%, p lt 0.01 and 50%, p lt 0.01, respectively) comparing with young controls. In addition, a strong (R-2 = 0.873, n = 6) and significant (p lt 0.01) correlation between the frequencies of PCD and DNA damage was found in all examined groups. We may conclude that the increases in both parameters evaluated in this study are not only associated with normal ageing processes, but are markedly and significantly intensified in AD pathogenesis. Thus, our data support the view that AD is a generalized systemic disease, at least as for the increased DNA damage and PCD incidence in peripheral blood cells. copyright", publisher = "Karger, Basel", journal = "Neurodegenerative Diseases", title = "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division", volume = "12", number = "3", pages = "156-163", doi = "10.1159/000346114" }
Živković, L., Potparević, B., Siedlak, S. L., Perry, G., Plećaš-Solarović, B., Milicević, Z.,& Bajić, V.. (2013). DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases Karger, Basel., 12(3), 156-163. https://doi.org/10.1159/000346114
Živković L, Potparević B, Siedlak SL, Perry G, Plećaš-Solarović B, Milicević Z, Bajić V. DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division. in Neurodegenerative Diseases. 2013;12(3):156-163. doi:10.1159/000346114 .
Živković, Lada, Potparević, Biljana, Siedlak, Sandra L., Perry, George, Plećaš-Solarović, Bosiljka, Milicević, Zorana, Bajić, Vladan, "DNA Damage in Alzheimer Disease Lymphocytes and Its Relation to Premature Centromere Division" in Neurodegenerative Diseases, 12, no. 3 (2013):156-163, https://doi.org/10.1159/000346114 . .