Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis
Samo za registrovane korisnike
1999
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Nitrite and nitrate levels in physiological fluids are commonly used as an index of nitric oxide production. We developed simple and rapid method for the determination of these anions by capillary zone electrophoresis employing berate buffer (pH 10, 100 mmol/l) as running electrolyte. The anions were analyzed in plasma and cerebrospinal fluid (CSF) without deproteinization of the samples. Electrophoresis was carried out in a capillary (36.5 cm X 75 mu m) at a potential of 15 kV, with on-column UV detection at 214 nm. Mean retention times for nitrite and nitrates were 4.631 and 5.152 min, respectively. The method was linear (r = 0.999) within a 1-500 mu mol/l concentration range. Physiological levels of nitrate in plasma (40.2 mu mol/l) and CSF (15.3 mu mol/l) could be determined with good precision (coefficients of variation lt 6%) and accuracy (recoveries of added nitrate to plasma and CSF were 97.4 and 104.5%, respectively). Measurements of the physiological levels of nitrite in pla...sma (6.1 mu mol/l) and CSF (0.9 mu mol/l) were less precise and accurate.
Ključne reči:
Nitrite / NitrateIzvor:
Journal of Chromatography B, 1999, 727, 1-2, 73-79Izdavač:
- Elsevier Science BV, Amsterdam
DOI: 10.1016/S0378-4347(99)00054-7
ISSN: 0378-4347
PubMed: 10360424
WoS: 000080170000009
Scopus: 2-s2.0-0032899663
Institucija/grupa
PharmacyTY - JOUR AU - Zunić, G AU - Spasić, S AU - Jelić-Ivanović, Zorana PY - 1999 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/221 AB - Nitrite and nitrate levels in physiological fluids are commonly used as an index of nitric oxide production. We developed simple and rapid method for the determination of these anions by capillary zone electrophoresis employing berate buffer (pH 10, 100 mmol/l) as running electrolyte. The anions were analyzed in plasma and cerebrospinal fluid (CSF) without deproteinization of the samples. Electrophoresis was carried out in a capillary (36.5 cm X 75 mu m) at a potential of 15 kV, with on-column UV detection at 214 nm. Mean retention times for nitrite and nitrates were 4.631 and 5.152 min, respectively. The method was linear (r = 0.999) within a 1-500 mu mol/l concentration range. Physiological levels of nitrate in plasma (40.2 mu mol/l) and CSF (15.3 mu mol/l) could be determined with good precision (coefficients of variation lt 6%) and accuracy (recoveries of added nitrate to plasma and CSF were 97.4 and 104.5%, respectively). Measurements of the physiological levels of nitrite in plasma (6.1 mu mol/l) and CSF (0.9 mu mol/l) were less precise and accurate. PB - Elsevier Science BV, Amsterdam T2 - Journal of Chromatography B T1 - Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis VL - 727 IS - 1-2 SP - 73 EP - 79 DO - 10.1016/S0378-4347(99)00054-7 ER -
@article{ author = "Zunić, G and Spasić, S and Jelić-Ivanović, Zorana", year = "1999", abstract = "Nitrite and nitrate levels in physiological fluids are commonly used as an index of nitric oxide production. We developed simple and rapid method for the determination of these anions by capillary zone electrophoresis employing berate buffer (pH 10, 100 mmol/l) as running electrolyte. The anions were analyzed in plasma and cerebrospinal fluid (CSF) without deproteinization of the samples. Electrophoresis was carried out in a capillary (36.5 cm X 75 mu m) at a potential of 15 kV, with on-column UV detection at 214 nm. Mean retention times for nitrite and nitrates were 4.631 and 5.152 min, respectively. The method was linear (r = 0.999) within a 1-500 mu mol/l concentration range. Physiological levels of nitrate in plasma (40.2 mu mol/l) and CSF (15.3 mu mol/l) could be determined with good precision (coefficients of variation lt 6%) and accuracy (recoveries of added nitrate to plasma and CSF were 97.4 and 104.5%, respectively). Measurements of the physiological levels of nitrite in plasma (6.1 mu mol/l) and CSF (0.9 mu mol/l) were less precise and accurate.", publisher = "Elsevier Science BV, Amsterdam", journal = "Journal of Chromatography B", title = "Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis", volume = "727", number = "1-2", pages = "73-79", doi = "10.1016/S0378-4347(99)00054-7" }
Zunić, G., Spasić, S.,& Jelić-Ivanović, Z.. (1999). Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis. in Journal of Chromatography B Elsevier Science BV, Amsterdam., 727(1-2), 73-79. https://doi.org/10.1016/S0378-4347(99)00054-7
Zunić G, Spasić S, Jelić-Ivanović Z. Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis. in Journal of Chromatography B. 1999;727(1-2):73-79. doi:10.1016/S0378-4347(99)00054-7 .
Zunić, G, Spasić, S, Jelić-Ivanović, Zorana, "Simple and rapid method for the measurement of nitrite and nitrate in human plasma and cerebrospinal fluid by capillary electrophoresis" in Journal of Chromatography B, 727, no. 1-2 (1999):73-79, https://doi.org/10.1016/S0378-4347(99)00054-7 . .