Premature centromere separation syndromes: A manifestation of genome instability?
Apstrakt
Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.
Izvor:
Balkan Journal of Medical Genetics, 2000, 3, 3, 19-22Izdavač:
- Macedonian Academy of Sciences and Arts
Institucija/grupa
PharmacyTY - JOUR AU - Potparević, Biljana AU - Bajić, V PY - 2000 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/277 AB - Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS. PB - Macedonian Academy of Sciences and Arts T2 - Balkan Journal of Medical Genetics T1 - Premature centromere separation syndromes: A manifestation of genome instability? VL - 3 IS - 3 SP - 19 EP - 22 UR - https://hdl.handle.net/21.15107/rcub_farfar_277 ER -
@article{ author = "Potparević, Biljana and Bajić, V", year = "2000", abstract = "Premature centromere separation (PCS) syndromes strongly support evidence that spatial and temporal organization of the cell cycle is crucial for maintaining genomic stability. To see if PCS as a genomic instability state can predispose cells to aneuploidy we have exposed lymphocytes to a anti-tumor agents Mitomycin C in a dose of 0,05 μM/ml; 0,15 μM/ml and 0,6 μM/ml, 8-CI-cAMP in a dose of 1 μM/ml; 5 μM/ml and 15 μM/ml and Taxol in a dose of 0,01 μM/ml; 0,05 μM/ml and 0,2 μM/ml or 24h using the CB-micronucleus test. Micronuclei were analyzed in 1000 bi-nuclear cells for each experimental and control group, thus Mitomycin C, Taxol and 8-Cl-cAMP induced an increase in the frequency of micronuclei in cells that where previously in the state of expressing PCS.", publisher = "Macedonian Academy of Sciences and Arts", journal = "Balkan Journal of Medical Genetics", title = "Premature centromere separation syndromes: A manifestation of genome instability?", volume = "3", number = "3", pages = "19-22", url = "https://hdl.handle.net/21.15107/rcub_farfar_277" }
Potparević, B.,& Bajić, V.. (2000). Premature centromere separation syndromes: A manifestation of genome instability?. in Balkan Journal of Medical Genetics Macedonian Academy of Sciences and Arts., 3(3), 19-22. https://hdl.handle.net/21.15107/rcub_farfar_277
Potparević B, Bajić V. Premature centromere separation syndromes: A manifestation of genome instability?. in Balkan Journal of Medical Genetics. 2000;3(3):19-22. https://hdl.handle.net/21.15107/rcub_farfar_277 .
Potparević, Biljana, Bajić, V, "Premature centromere separation syndromes: A manifestation of genome instability?" in Balkan Journal of Medical Genetics, 3, no. 3 (2000):19-22, https://hdl.handle.net/21.15107/rcub_farfar_277 .