The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets
Само за регистроване кориснике
2001
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were gr...eater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.
Извор:
Journal of Pharmaceutical and Biomedical Analysis, 2001, 24, 5-6, 1019-1025Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(00)00536-7
ISSN: 0731-7085
PubMed: 11248497
WoS: 000167764200032
Scopus: 2-s2.0-0035096691
Институција/група
PharmacyTY - JOUR AU - Zečević, Mira AU - Živanović, L AU - Agatonović-Kuštrin, Snežana AU - Minić, D PY - 2001 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/293 AB - A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Journal of Pharmaceutical and Biomedical Analysis T1 - The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets VL - 24 IS - 5-6 SP - 1019 EP - 1025 DO - 10.1016/S0731-7085(00)00536-7 ER -
@article{ author = "Zečević, Mira and Živanović, L and Agatonović-Kuštrin, Snežana and Minić, D", year = "2001", abstract = "A multifactor optimisation technique is successfully applied to develop a new HPLC method in which methyldopa, hydrochlorothiazide and amiloride were analysed and determined on a C18 column with detection at 286 nm. The optimal conditions of HPLC separation were determined with the aid of the response surface diagram - 'window diagram'. The effect of simultaneously varying the pH, proportion aqueous acetic acidum and methanol in the mobile phase were studied to optimise the separation. The mobile phase composition that provides an acceptable resolution methyldopa, hydrochlorothiazide and amiloride in a short elution time is water-methanol (75:25) and pH 3.60. The k' values for methyldopa, hydrochlorothiazide and amiloride after optimisation were 1.40, 2.50 and 5.33, respectively. Relative retention (a) for ratio hydrochlorothiazide/methyldopa and amiloride/hydrochlorothiazide were 1.767 and 2.159, respectively. Correlation coefficients of the calibration curves for all analytes were greater than 0.995 and the R.S.D. values for the slope and the intercept with respect to the linearity were less than 2%. A method is applied for the quantitative analysis of Aatan(R) tablets (Lek-Ljubljana). The powdered tablets are extracted with methanol, containing caffeine as the internal standard and assayed by comparison of peak areas after liquid chromatography. The high recovery (for all analytes about 100%) and the low R.S.D. ( lt 2%) confirm good precision and reproducibility of the chromatographic method.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Journal of Pharmaceutical and Biomedical Analysis", title = "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets", volume = "24", number = "5-6", pages = "1019-1025", doi = "10.1016/S0731-7085(00)00536-7" }
Zečević, M., Živanović, L., Agatonović-Kuštrin, S.,& Minić, D.. (2001). The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis Pergamon-Elsevier Science Ltd, Oxford., 24(5-6), 1019-1025. https://doi.org/10.1016/S0731-7085(00)00536-7
Zečević M, Živanović L, Agatonović-Kuštrin S, Minić D. The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets. in Journal of Pharmaceutical and Biomedical Analysis. 2001;24(5-6):1019-1025. doi:10.1016/S0731-7085(00)00536-7 .
Zečević, Mira, Živanović, L, Agatonović-Kuštrin, Snežana, Minić, D, "The use of a response surface methodology on HPLC analysis of methyldopa, amiloride and hydrochlorothiazide in tablets" in Journal of Pharmaceutical and Biomedical Analysis, 24, no. 5-6 (2001):1019-1025, https://doi.org/10.1016/S0731-7085(00)00536-7 . .